Effect of irrigation with nutrient solutions mixed with treated wastewater on Asiatic lily ‘ Brunello ’ grown in a closed soilless culture

A plastic greenhouse study was implemented to evaluate the potential use of treated wastewater for irrigation of Asiatic lily ‘Brunello’ grown in zeolite. Plants received the following treatments: a nutrient solution (N) alone, (N) mixed with treated wastewater (W) at rates of 3N:1W, 1N:1W and 1N:3W respectively. A closed system was used in which the drain solution was circulated for several days until its electrical conductivity reached 2.3 dS m, after which fresh irrigation solutions were used to start a second cycle of circulation. The results indicated that plants irrigated with (N) or 3N:1W solution had the longest stems (34.4 and 36.2 cm) respectively, peduncles and buds (about 4.5 cm), and the greatest shoot (3.4 and 3.8 cm) and bud mass (14.95 and 17.6 g) respectively. Plants irrigated with 1N:3W solution had the highest dry mass tissue content of K (3.06 g kg ) and B (35.5 mg kg). Plants irrigated with 1N:1W or 1N:3W were inferior to other plants. It can be concluded that 1N:3W mixture may be used for production of high quality cut flowers of lily. Moreover, it is expected to save 3.4 litres m of a nutrient solution and 1850, 347 and 1870 mg m day for N, P, and K respectively.


INTRODUCTION
Closed soilless culture is used to conserve water, increase fertilizer use efficiency, and reduce nutrient leaching into the environment.However, continual use of the drain solution in closed cultures may result in increased salinity in the circulating solution (Baas et al., 1995;Karam and Al-Daood, 2005;Sonneveld and Van der Burg 1991;Sonneveld et al., 1999) and nutritional imbalances (Savvas, 2001).So, management of agricultural water becomes very important (Maryam et al., 2015;Mohammad, 2012a;Mohammad, 2012b;Stavros et al., 2015).To reduce consumption of potable water in agriculture, treated wastewater has been used as an alternative source of irrigation water which may also compensate for partial fertilizer requirements (Karam et al.,2009) and provide adequate amount of nutrients (Khan et al., 2009) for some plants.Treated wastewater has shown to have no adverse effect on rose (Rosa hybrida) (Bernstein et al., 2006) and to improve quality and yield of ornamental plants including croton (Codiaeum variegatum Blume) (Karam et al., 2006), lily (Lilium 'Aziatische Group ') (Safi et al., 2007a) and gerbera (Gerbera jamesonii Bolus ex Hooker f.) (Damasceno et al., 2010).However, treated wastewater was found to increase salinity of soil (Hussain et al., 2002;Safi et al., 2007b).Salinity affects plant growth through osmotic potential of the soil solution, nutritional imbalance and/or specific ion effects (Shannon, 1998).Nutritional imbalances are related to nutrient availability, competitive uptake, and transport or partitioning within the plant (Grattan and Grieve, 1994).In fact, salinity was reported to reduce growth and yield of cut flowers (Baas et al., 1995;Sonneveld and Van der Burg, 1991) including lily (Daood and Karam, 2007;Karam and Al-Daood, 2005;Morgan, 2006;Sonneveld et al., 1999).
To overcome salinity issues, zeolites, a microporous aluminosilicate mineral, has been commonly used as growing medium in plant production.Zeolites have the ability to mitigate the salt damage of plants by exchanging Na in water for Ca of the zeolite (Yasuda et al., 1998).So, they may be used when saline water is used for irrigation.Occlusion of NaCl from aqueous solutions occurs in zeolite since the surface conductivity of zeolite increases in salt solutions due to the ion pairs of salt adsorbed onto the hydroxyl groups of the zeolite surface (Breck, 1974), consequently, preventing excessive Na uptake by the plant and reducing its toxicity (Qian et al., 2001).Incorporation of zeolitic tuff into peat-perlite was recommended to reduce substrate EC and to offset the adverse effect of salinity associated with treated wastewater, rendering the substrate more favourable for growth of croton (Karam et al., 2006;Karam et al., 2009).
Closed soilless culture was reported to reduce water and fertilizer use for Asiatic lily 'Zsa Zsa' in zeollitic tuff without affecting plant performance (Karam and Al-Daood, 2005).Treated wastewater has also been used for cut flowers in open soilless culture (Safi et al., 2007b) and in soil (Safi et al., 2005;Safi et al., 2007b).However, there are no documented studies on irrigation of hybrid lily with treated wastewater in a closed soilless culture.Using treated wastewater as an alternative source of irrigation water would minimize the use of fresh water.The objective of the current study is to evaluate the potential use of treated wastewater for irrigation of Asiatic lily 'Brunello' in a closed soilless culture.

MATERIALS AND METHODS
The experiment was conducted in Jordan University of Science and Technology-Jordan (JUST), inside a plastic greenhouse with average daily temperature of 25±3 ºC, relative humidity (RH) of 35 %, photosynthetic photon flux density (PPFD) of 97 µmol m -2 s -1 , and natural photoperiod.
Asiatic lily 'Brunello' bulbs (12-14 cm circumference) were planted 10 cm deep in each growing bed at 25 x 25 cm planting space.The bulbs were irrigated with tap water for two weeks before starting the treatments.The experiment was conducted in a completely randomized design with four replicates (growing beds) per treatment and 24 plants per bed.
A nutrient solution (N) was prepared by diluting 2 l of each of three stock nutrient solutions in 400 l tap water.The first stock solution contained (per liter) 112.5 g KNO 3 , 31.5 g (NH 4 ) 2 SO 4 , 0.49 g ZnSO 4 , 0.48 g MnCl 2 and 0.035 g CuSO 4 , the second contained 112.5 g KNO 3 and 6.1 g Fe(NO 3 ) 3 .9H 2 O and the third contained 30.12 ml H 3 PO 4 and 116.65 g Ca(NO 3 ) 2 .The treatments consisted of four irrigation solutions: the nutrient solution (N), singly or mixed with secondary treated wastewater (W) at rates of 3N:1W, 1N:1W or 1N:3W.Tap water had EC of 1.27 dS m -1 , pH 8.2 and treated wastewater had EC of 2.48 dS m -1 and pH 7.9.Chemical analysis of the irrigation solutions is presented in Table 1.
The irrigation solutions were injected separately using a submerged pump in 400 l tank per treatment into a GR drip irrigation system with 8 drippers per growing bed and 4 l h -1 dripper discharge.The plants were automatically irrigated with the irrigation solutions three times a day at 8.00, 12.00 and 18.00 o'clock for 15 min each.At the end of the day, the drain solutions from the four replicate beds of each treatment were collected, mixed and the volume was measured.The drain solution was then returned to the corresponding tank to be mixed with the irrigation solution to form the supply solution which was circulated the next day.The EC and pH of the supply and drain solutions were measured once daily using a manual EC meter (Omega TDH-5031, USA) and pH meter (Omega PHH-5012, USA).Irrigation scheduling and drainage management were repeated daily until the drain solution reached a threshold EC (2.3 dS m -1 ), after which the supply solution in the tank was replaced with a fresh irrigation solution to start a new cycle of circulation.This strategy was adopted until the end of the experiment.The volume of the circulating solution supplied to each treatment / day / m 2 was calculated as follows: [4 growing beds * 8 drippers * 4 l h -1 dripper discharge * 45 min irrigation duration] / [4 growing beds * 3 m long * 0.3 m width] = 26.7 l.
At the end of the experiment (40 days after planting), the plants were harvested when the first flower was fully coloured, but not yet open.The plants were dug out and the tuff particles around the roots were carefully removed keeping the root system intact.Data were recorded on stem length (from bulb tip to peduncle base) and diameter, leaf number, root length (from bulb base to the longest root tip), shoot (stem and leaves) and root fresh and dry (oven dried at 70 ºC for 48 h) mass, peduncle length and bud number, length and mass.Tissue analysis was performed for determination of concentrations of macro, micro and heavy metal elements.The leaves were dried and ground using a laboratory mill (Thomas Scientific, USA) to pass through a 0.5-mm sieve.The leaves were analysed for total nitrogen using Kjeldahl digestion (Nelson and Sommers, 1982).The remaining ground leaves were subjected to dry ash digestion at 550 °C for 2 h.The cooled ash was moisturized in 3-4 drops of diluted HNO 3 (1:1) on a heating plate until HNO 3 completely evaporated.The aliquot was used to determine concentration of P by ammonium molybdate-vandate method (Chapman and Pratt, 1961) using a spectrophotometer (Genesys 10, USA).Analysis of K, Ca, Mg, Na, Cl, Fe, Zn, Mn, Cu and B was performed as described by the Association of Official Analytical Chemists (Horwitz, 2000).The remaining ash was dissolved in diluted HCl (1:1) and put in lanthanum and lithium solutions.The aliquots were analysed for K and Na using a flame photometer (Sherwood Scientific Ltd 410, UK) and Ca, Mg, Fe, Zn, Mn, Cu and B by atomic absorption spectroscopy (Varian model, Agilent Technologies Co., USA).Chloride was determined by titration using 0.05N AgNO 3 (Richards, 1954.).Concentrations of Cd, Cr, Ni and Pb were determined using microwave digestion system (Microwave Digestion Lab Station MD 01, Milestone S.r.L., Italy) and Solar 969 Atomic Absorption spectroscopy (TJA Solution co., UK) (Rechcigl and Payne, 1990).

Net daily water consumption was calculated according to the following equations:
First equation: A = B -D Where A is the net water consumption (m 3 ), B is the quantity of applied water (m 3 ), and D is the quantity of drain water (m 3 ).

Second equation: Y = A x P x C
Where Y is the quantity of the nutrient taken by plant from treated wastewater (g), A is the net water consumption (m 3 ), P is the percentage of wastewater in nutrient solution, and C is the concentration of a certain nutrient (N, P, and/or K) (g m -3 ).

Third equation: WUE = BY/ A
Where WUE is water use efficiency (kg m -3 ), BY is the biological yield (shoots and flowers) (kg), and A is the net water consumption (m -3 ).In all calculations, the volume of discharged solutions after being replaced with fresh ones was not considered.
In addition, biomass water use efficiency (WUE) was calculated for shoot fresh mass and flower mass (bud number x bud mass) for each treatment as follows: average shoot fresh mass or flower mass per plant at harvest / total volume of irrigation solution supplied per plant from the first day of treatment application until harvest.

Statistical analysis
Data were subjected to analysis of variance by the General Linear Models procedure using SAS (Statistical Analysis System, version 9.1, 2002).Mean comparison was performed using the Least Significant Difference (LSD) method at P ≤ 0.05.

Plant growth and flowering
Stem diameter, leaf number, root length or bud number was not affected by the irrigation solution (Table 2).With the exception of root mass, all other parameters were significantly greater in plants irrigated with N or 3N:1W solution than in plants irrigated with 1N:1W or 1N:3W solution (Table 2, Fig. 1).Plants irrigated with 1N:3W solution had the least root fresh and dry mass.

Plant tissue analysis
Leaf analysis revealed that concentrations of only K, Na, Fe and B were affected by the irrigation solution (Table 3).Plants receiving N, 3N:1W or 1N:3W solution had the highest concentrations of K (3.28, 3.23 or 3.06 g kg -1 dry mass, respectively) and B (75.4, 64.4 or 53.5 mg kg -1 dry mass, respectively).Furthermore, the highest level of Na (0.90, 0.94 or 0.99 g kg -1 dry mass) was detected in plants irrigated with 3N:1W, 1N:1W or 1N:3W, respectively.Plants irrigated with 3N:1W or 1N:3W had the highest level of Fe (862 or 694 mg kg -1 dry mass).There was no significant effect of the irrigation solution on tissue content of the heavy metal Cd, Cr, Ni or Pb (Table 3).

Electrical conductivity and pH of the supply and drain solutions
Two cycles of recirculation of the irrigation solutions were performed throughout the experiment (Fig. 2-5).At the start of the first cycle, the solutions N, 3N:1W, 1N:1W and 1N:3W had EC of 1.65, 1.73, 1.91 and 2.12 dS m -1 and pH of 6.58, 7.72, 7.92 and 8.01, respectively (Table 1).
At the start of circulation, the drain solutions from beds receiving N, 3N:1W, 1N:1W or 1N:3W had EC of 1.62, 1.68, 1.87 or 2.09 dS m -1 and pH of 6.65, 7.79, 7.98 or 8.07, respectively.The threshold EC (2.3 dS m -1 ) of the drain solutions for the treatments N, 3N:1W, 1N:1W and 1N:3W was attained after 18, 17, 15 and 12 days of circulation, respectively.In the second cycle, the threshold EC was attained (after 13 days) only when 1N:3W was used because the experiment was terminated, thus was not long enough to reach the target EC when the other irrigation solutions were used.Throughout the experiment, the average EC of the supply solution was 1.76, 1.81, 1.96 and 2.09 dS m -1 (Fig. 2) and of the drain solution was 1.77, 1.82, 1.97 and 2.11 dS m -1 for the treatments N, 3N:1W, 1N:1W and 1N:3W, respectively (Fig. 3).
In both cycles, EC of all supply (circulating) and drain solutions decreased in the first few days of circulation, then increased and the solution 1N:3W and its drain had the highest EC followed by 1N:1W (Fig. 2 and 3).Moreover, EC values of the supply solution 3N:1W or its drain were not different from those of the solution N or its drain in the first cycle, but were higher in the second cycle.pH of supply (Fig. 4) and drain (Fig. 5) solutions increased in both cycles.At the end of the experiment, pH of the supply solutions N, 3N:1W, 1N:1W and 1N:3W was 7.22, 8.44, 8.52 and 8.97 and of the drain solutions was 7.29, 8.49, 8.57 and 9.05, respectively.In both cycles, the supply solution N and its drain had the lowest pH.

Savings in irrigation solutions and biomass water use efficiency
Savings in the nutrient solution N were 10.1 l m -2 day -1 and in the nutrients N, P and K were 1850, 347 and 1870 mg m -2 day -1 respectively.The highest savings were recorded when 1N:3W solution was used (Table 4).It was observed that biomass WUE was 1.16, 1.25, 1.04 or 1.06 kg m -3 for shoot fresh mass and 2.0, 2.37, 1.39 or 1.56 kg m -3 for flower mass, when N, 3N:1W, 1N:1W or 1N:3W solutions were used, respectively.Consumed irrigation solution u (l m -2 day -1 ) 13.7 NS 13.5 NS 13.4 NS 13.4NS Consumed N solution w (l m -2 day -1 ) 13. Treated wastewater had potential as irrigation water for production of Asiatic lily, when mixed with potable water.However, high percentage of treated wastewater was a limiting factor.Mixing a nutrient solution, usually recommended for Asiatic lily, with treated wastewater at a ratio of 1:1 or 1:3 did not prove to be feasible since the plants exhibited growth retardation (Table 2).This may be attributed to exposure of the plants to salinity stress because of the high EC of the circulating solution (Fig. 2) and the drain solution (Fig. 3) around the roots.This is in agreement with studies which indicated that continual reuse of the drain solution in closed soilless culture usually increases EC of the circulating solution (Baas and Van den Berg, 1999;Bar-Yosef et al., 2001;Daood and Karam, 2007;Karam and Al-Daood, 2005).This effect was evident in the current study when the nutrient solution N was used alone for irrigation (Fig. 2).However, the problem of elevated EC was aggravated when 1N:1W or 1N:3W was used.This may be explained by the high concentrations of Na, Cl and HCO 3 in treated wastewater which rendered the initial EC of 1N:1W and 1N:3W high (1.91 and 2.12 dS m -1 , respectively) (Table 1) and may have resulted in accumulation of Na and Cl in the root zone (Karam et al., 2006;Karam et al., 2009).Plant growth is adversely affected under salt stress due to osmotically induced water stress, specific ion toxicity due to high concentration of Na + and Cl -, nutrient ion imbalance due to high levels of Na + and Cl -which reduce uptake of K + , NO 3 -and PO 4 3- (Greenway and Munns, 1980).
Accumulation of Na in the circulating solution in closed soilless culture may lead to reduced level of K in the circulating solution (Karam and Al-Daood, 2005;Savvas and Manos, 1999;Sonneveld, 1981) and the plant (Savvas et al., 2009).Accumulation of Na is expected to be more significant when zeolite is used as a substrate due to adsorption of K ions by zeolite after replacing Na ions resulting in less availability of K and higher concentration of Na in the substrate (Williams and Nelson, 1997).The problem of Na accumulation is aggravated when saline water is used in closed cultures (Pardossi et al., 2006).Reduced K uptake by the plant is attributed to the competition between K and Na for the absorption sites of the roots (Rusan et al., 2003).In the current study, the suppressive effect of Na on K uptake was evident from the lower K and higher Na contents in plants irrigated with 1N:1W or 1N:3W compared to those irrigated with N or 3N:1W (Table 3).In fact, K concentration in plants irrigated with the former solutions was less than the sufficiency range reported for Easter lily (3.3-5.0 %) (Jones et al., 1991).It is obvious that the competition was advantageous to Na when 1N:1W or 1N:3W solution was used due to the high initial Na and low initial K contents in those solutions (Table 1).
In addition to the negative effect of Na on K acquisition by the roots, high levels of Na under saline-sodic conditions may disrupt the integrity of root membranes and alter their selectivity from K to Na (Grattan and Grieve, 1999).According to the authors, the selectivity of the root system for K over Na must be sufficient to meet the levels of K required for metabolic processes, ion transport regulation and osmotic adjustment which affect water uptake.Fulfilling the need of plants for K may be achieved by increasing concentration of K in the irrigation solution.In the present study, the high initial content of K in the irrigation solutions (N) and 3N:1W (Table 1) was apparently sufficient to increase selectivity of the roots for K over Na, resulting in increased K and reduced Na contents in plants irrigated with those solutions (Table 3).
Only the plants that were irrigated with 1N:3W exhibited reduced root fresh mass, implying reduced water content, probably due to elevated EC (2.31 dS m -1 ) of the drain solution at the end of the experiment (Fig. 3).Those plants also exhibited reduced root dry mass implying reduced root mass, which occurs under salinity conditions (Shannon and Grieve, 1999), thus reduced water uptake.Results of the present study confirm findings of Karam and Al-Daood (2005) who reported reduction in root fresh mass of Asiatic lily 'Zsa Zsa' only when EC of the drain solution reached 2.2 dS m -1 and in root dry mass as EC increased up to 2.0 dS m -1 .Reduced water uptake by the plant may reduce Ca transport in the plant since the rate of transpiration is regarded as the main force for Ca transport to the leaves (Clarkson, 1984).However, retardation in growth of plants irrigated with 1N:1W or 1N:3W is not likely to be due limited Ca uptake since the plants were not significantly different from those irrigated with N or 3N:1W solution with respect to Ca content in the leaves (Table 3).
Electrical conductivity of the supply and drain solutions for all treatments decreased in the first 6 days of circulation (Fig. 2 and 3), which may be attributed to the high ion adsorption and cation exchange capacity of zeolitic tuff (Mumpton, 1983).The pH of all supply and drain solutions increased with increasing EC (Fig. 4 and 5), which confirms findings of Karam and Al-Daood (2005).
Increased pH in the root zone usually limits absorption of micronutrients.In the present study, pH of the drain solution increased above 8 for all treatments except for the solution (N), yet there was no effect of pH or EC on tissue contents of micronutrients other than Fe and B (Table 3), which may be due to the relatively short duration of circulation in each cycle.Our findings are in agreement with those reported for Asiatic lily 'Zsa Zsa' which revealed no effect of EC on tissue content of micronutrients except Mo (Karam and Al-Daood, 2005).
Tissue contents of only K, Na, Fe and B were significantly affected by the irrigation solution (Table 3).Similarity in tissue N content at all EC values implies lack of effect of EC on N uptake as was reported for Asiatic lily 'Zsa Zsa' (Karam and Al-Daood, 2005) with EC of 2.2 dS m -1 and petunia (Petunia × hybrid (Hook) Vilm.) and begonia (Begonia 'Semperflorens Cultorum Group') (James and van Iersel, 2001) even with EC of 3 dS m -1 .Tissue content of Fe for all treatments (Table 3) was substantially higher than the upper level of sufficiency range for Easter lily (60-200 ppm) (Jones et al., 1991), suggesting luxury consumption.Tissue contents of P, Ca, Mg, Zn, Cu and B for all treatments were within the sufficiency range for Easter lily (0.25-0.7 %, 0.6-1.5 %, 0.2-0.7 %, 20-200 ppm, 8-50 ppm and 25-75 ppm, respectively), whereas contents of N and Mn were lower than the sufficiency range (3.3-4.8 % N and 35-200 ppm Mn) (Jones et al., 1991).
Only plants irrigated with 3N:1W were comparable to those irrigated with (N) solution (Table 2).This may be explained by the similar EC conditions that the plants were exposed to for most part of the experiment (Fig. 2 and 3).Although the first cycle of circulation lasted for 18-19 days, the difference in EC between the two solutions or their drains was slight.In the second cycle, the difference in EC between the two solutions was large, but the cycle lasted for only 7-8 days.The threshold EC (2.3 dS m -1 ) of the drain solution of the treatments N, 3N:1W, 1N:1W and 1N:3W was attained after 18, 17, 15 and 12 days of circulation, respectively (Fig. 3).Accordingly, using the solution 1N:3W the replacement with a fresh solution every 12 days was necessary, whereas using N or 3N:1W solution required replacement with new solutions after 17-18 days.Karam and Al-Daood (2005) were able to achieve a target EC of 2.2 dS m -1 after 20 days of circulation of a nutrient solution in a closed soilless culture of Asiatic lily 'Zsa Zsa'.
Stem diameter, leaf number, root length or bud number was not affected by the treatment (Table 2), indicating that EC up to 2.3 dS m -1 did not affect those parameters.This confirms results obtained by Karam and Al-Daood (2005) which revealed no change in leaf number in Asiatic lily 'Zsa Zsa' at EC 1.6-2.2dS m -1 or in stem diameter or bud number at EC 1.8-2.2dS m -1 .In the current study, the plants irrigated with 1N:1W or 1N:3W were exposed to EC above 1.8 dS m -1 throughout the experiment (Fig. 2 and 3) and exhibited reduced stem length, shoot fresh mass and bud mass (Table 2), and those irrigated with 1N:3W exhibited reduced root fresh and dry mass.This implies that 'Brunello' cultivar is quite sensitive to salinity as was reported for other cultivars of Asiatic lily.For example, Karam and Al-Daood (2005) reported reduced stem length and diameter, shoot fresh and dry mass, peduncle length and bud number in 'Zsa Zsa' lily as EC of the drain solution increased from 1.6 to 1.8 dS m -1 and reduced stem length, shoot dry mass and peduncle length as EC increased from 1.8 to 2.0 dS m -1 .Daood and Karam (2007) also demonstrated that a rise of 0.48 dS m -1 in EC was accompanied with reductions of 10 % in stem length, 22 % in shoot mass, 32 % in root or bud mass, 13 % in peduncle length and 18 % in bud length in 'Zsa Zsa' lily.Furthermore, Morgan (2006) reported that the ideal EC for lily production in hydroponic cultures is 1.2-1.8dS m -1 and that higher EC levels resulted in slow growth, stunted plants and small flowers.Sonneveld et al. (1999) also reported that EC higher than 2.0 dS m -1 caused growth retardation in lily.
The results of this study can be expanded to other regions where ornamental plants are grown.This can be achieved when using soilless culture in a closed system and where treated wastewater is available.Since wastewater treatment plants exist almost everywhere, and plastic greenhouses are constructed widely in agricultural areas.Also, the soilless culture using zeolite has become very common.All these conditions make this study applicable to different regions and cultures.And good results seem to be achievable.

CONCLUSION
A nutrient solution mixed with treated wastewater at a ratio of 3:1 may be used to produce Asiatic lily 'Brunello' cut flowers in a closed soilless culture in which the plants are grown in zeolitic tuff and the drain solution is recycled until its EC reaches 2.3 dS m -1 .By using such a strategy, it is expected to save daily 3.4 l m -2 of the nutrient solution and achieve the highest biomass water use efficiency for shoot fresh mass and flower mass.This translates into savings in fresh water and fertilizers.This research is unique since it investigated for the first time the effect of using mixed treated wastewater on cut flowers.Also, the growing system was closed soilless culture using zeolite.
And the most common growing activities in the study area use treated wastewater in open fields to grow forage crops not cut flowers.The advantages of this research can be summarized in using water efficiently in regions where scarcity of water is a major problem such as Jordan.Moreover, nutrients can also be saved while producing high quality cut flowers.Future studies are needed to navigate the efficiency of using similar irrigation management practice in open fields.Furthermore, research can be made to grow different cut flowers and other ornamental plants.

Figure 1 :
Figure 1: Growth of Asiatic lily 'Brunello' as influenced by irrigation with different ratios of a nutrient solution (N) and treated wastewater (W).

Figure 2 :
Figure 2: Changes in EC of the supply solution with time of circulation as influenced by irrigation Asiatic lily 'Brunello' with different ratios of a nutrient solution (N) and treated wastewater (W).

Figure 3 and Figure 2 :
Figure 3 and Figure 2: Changes in EC of the drain solution with time of circulation as influenced by irrigation Asiatic lily 'Brunello' with different ratios of a nutrient solution (N) and treated wastewater (W).

Figure 4 :
Figure 4: Changes in pH of the supply solution with time of circulation as influenced by irrigation Asiatic lily 'Brunello' with different ratios of a nutrient solution (N) and treated wastewater (W).

Figure 5 :
Figure 5: Changes in pH of the drain solution with time of circulation as influenced by irrigation Asiatic lily 'Brunello' with different ratios of a nutrient solution (N) and treated wastewater (W).

uwxy
Volume of supplied irrigation solution -volume of drainage.Volume of consumed irrigation solution * % (N) solution in irrigation solution.Volume of consumed irrigation solution * % (W) in irrigation solution.Amount of nutrient consumed using (N) solution -amount of nutrient consumed using the considered irrigation solution, which = consumed irrigation solution * proportion of (N) solution in the considered irrigation solution * concentration of the nutrient in (N) solution.z Means within rows having different letters are significantly different according to LSD (P ≤ 0.05).NS means non-significant within rows.

Table 1 :
Chemical analysis of the irrigation solutions prepared from a nutrient solution (N), singly or mixed with treated wastewater (W) at 3:1, 1:1 or 1:3

Table 2 :
Growth and flowering of Asiatic lily 'Brunello' as influenced by irrigation with different ratios of a nutrient solution (N) to treated wastewater (W) in a closed soilless culture Means within rows having different letters are significantly different according to LSD (P ≤ 0.05).Values are average of 10 plants.NS means non-significant within rows. z

Table 3 :
Concentrations of elements in the leaves of Asiatic lily 'Brunello' as influenced by irrigation with different ratios of a nutrient solution (N) to treated wastewater (W) in a closed soilless culture.

Table 4 :
Effect of irrigation of Asiatic lily 'Brunello' with different ratios of a nutrient solution (N) to treated wastewater (W) on average savings in the irrigation solutions and the nutrients N, P and K due to the use of (W)