Effects of spermine and putrescine polyamines on capsaicin accumulation in Capsicum annuum L. cell suspension cultures

Esra KOÇ, Cemil İŞLEK, Belgizar KARAYİĞİT


This study examined the effects of different concentrations of spermine (Spm) and putrescine (Put) elicitors on capsaicin production at different times in cell suspension culture of peper (Capsicum annuum L‘Kahramanmaraş Hat-187’.), raised from pepper seeds. Callus was obtained from hypocotyl explants of pepper seedlings germinated in vitro conditions, and cell suspensions were prepared from calluses. Spm (0.1, 0.2 and 0.4 mg l-1) and Put (0.1, 0.2 and 0.4 mg l-1) elicitors were applied on cell suspensions, and control groups free from elicitor treatment were created. The amount of capsaicin in cells was found to be higher in the control groups and samples treated with Spm elicitors when compared to filtrates. The highest increase in the capsaicin amount in cells was determined on day 12 of elicitation with 0.2 mg l-1 Spm application. The highest capsaicin amount passing into the filtrate was determined as 0.1 mg l-1 Spm on day 8. The most effective Put concentration and time on capsaicin amount were found as 0.2 mg l-1 Put on day 12 in both cells and filtrates. The highest total capsaicin was also determined in the 0.2 mg l-1 Spm application on day 12with 312.747 ± 8.70 µg  g-1 of culture. Exogenous treatment of Spm and Put elicitors affected capsaicin accumulation.


capsaicin; Capsicum annuum L.; cell filtrate; pepper; polyamines

Full Text:



Ahern, G.P., Wang, X., Miyares, R.L. (2006). Polyamines are potent ligands for the capsaicin receptor TRPV1. The Journal of Biological chemistry, 281(13), 8991-8995. https://doi:10.1074/jbc.M513429200

Brooks, C.J.W., Watson, D.G., Freer, I.M. (1986). Elicitation of capsidiol accumulation in suspended callus cultures of Capsicum annuum. Phytochemistry, 27(5), 1089-1092. https://doi.org/10.1016/S0031-9422(00)81559-9

Chen, D., Shao, Q., Yin, L., Younis, A.,Zheng, B. (2018). Polyamine function in plants: metabolism, regulation on development, and roles in abiotic stress responses. Frontiers Plant Science, 9, 1945. https://doi: 10.3389/fpls.2018.0194

Ellialtıoğlu, Ş., Üstün, A.S., Mehmetoğlu, Ü. (1998). Bazı biber çeşitlerinde in vitro Kallus oluşumu için en uygun besin ortamı bileşiminin belirlenmesi. II-Kızılırmak Uluslararası Fen Kongresi, pp. 51-58, Kırıkkale.

Harishchandra, B.G., Parvatam, G., Ravishankar, G.A. (2012). Laminarin as a potential non-conventional elicitor for enhancement of capsaicinoid metabolites. Asian Journal of Plant Science and Research, 2 (4), 490- 495.

İşlek, C., Üstün, A.S., Koç, E. (2014). The effects of cellulase on capsaicin production in freely suspended cells and immobilized cell cultures of Capsicum annuum L. Pakistan Journal of Botany, 46(5), 1883-1887.

Johnson, T.S., Ravishankar, G.A., Venkataraman, L.V. (1990). In vitro capsaicin production by immobilized cells and placental tissues of Capsicum annuum L. grown in liquid medium. Plant Science, 70 (2), 223-229. https://doi.org/10.1016/0168-9452(90)90137-D

Lindsey, K. (1985). Manipulation by nutrient limitation of the biosynthetic activity of immobilized cells of Capsicum frutescens Mill. cv. annuum. Planta, 165, 126-133. https://doi:10.1007/BF00392221

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.

Puyang, X., An, M., Han, L., Zhang, X. (2015). Protective effect of spermidine on salt stress induced oxidative damage in two Kentucky bluegrass (Poa pratensis L.) cultivars. Ecotoxicology and Environmental Safety, 117, 96-106. https://doi10.1016/j.ecoenv.2015.03.023

Palacio, J.J.R. (1977). Spectrophotometric determination of capsaicin. Journal Association of Official Analytical Chemists, 60, 970-972.

Palacio, J.J.R. (1979). Further study of the spectrophotometric determination of capsaicin. Journal-Association of Official Analytical Chemists, 62, 1168-1170.

Ramirez, E.K., Vidal, L.H., Hidalgo, D., Moyano, E., Golenioswki, M., Cusidó, R., Palazon, J. (2016). Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules, 21(2), 182. https://doi:10.3390/molecules21020182

Ravishankar, G.A., & Venkataraman, L.V. (1990). Food applications of plant cell cultures. Current Science, 59, 914-920. https://www.jstor.org/stable/24094869

Sukrasno, N., & Yeoman, M.M. (1993). Phenylpropanoid metabolism during growth and development of Capsicum frutescens fruits. Phytochemistry, 32, 839-844.

Sudha, G., & Ravishankar, G. A. (2003a). Putrescine facilitated enhancement of capsaicin production in cell suspension cultures of Capsicum frutescens. Journal of Plant Physiology, 160, 339-346. https:// doi:10.1078/0176-1617-00928

Sudha, G., & Ravishankar, G. A. (2003b). Influence of methyl jasmonate and salicylic acid in the enhancement of capsaicin production in cell suspension cultures of Capsicum frutescens Mill. Current Science, 85(8), 1212-1217. https://www.jstor.org/stable/24108623

Tang, W., Harris, L.C., Outhavong, V., Newton, R.J. (2004). Antioxidants enhance in vitro plant regeneration by inhibiting the accumulation of peroxidase in Virginia pine (Pinus virginiana Mill.). Plant Cell Reports, 22(12), 871-877. https://doi:10.1007/s00299-004-0781-3

Vuosku, J., Karppinen, K., Muilu-Mäkelä, R., Kusano, T., Sagor, G. H. M., Avia, K. (2018). Scots pine aminopropyltransferases shed new light on evolution of the polyamine biosynthesis pathway in seed plants. Annals of Botany, 121, 1243-1256. https://doi:10.1093/aob/mcy012

DOI: http://dx.doi.org/10.14720/aas.2020.115.2.1199


  • There are currently no refbacks.

Copyright (c) 2020 Esra Koç, Cemil İşlek, Belgizar Karayiğit

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Acta agriculturae Slovenica is an Open Access journal published under the terms of the Creative Commons CC BY License.


eISSN 1854-1941