Effect of foliar or soil application of selenium on some morphological and physiological traits of garden pansy (Viola x wittrockiana Gams) grown under salinity stress



Salinity stress is one of the most important plant stresses in Iran. In this regard, a factorial experiment was conducted to investigate the effects of salinity stress on the garden pansy. The investigated factors were containing sodium selenate (0, 2, 4 and 8 mg l-1), its method of application (foliar and soil applications) and salinity stress (0, 3 and 6 dS m-1). The obtained results indicated that salinity leads to the significant reduction in morphological traits, chlorophyll a and b contents. Under the salinity of 6 dS m-1, when sodium selenate was used in the soil, the fresh and dry mass of flower increased by 11.34 and 10.39 %, respectively, compared to the control. However, the use of sodium selenate by foliar application led to the increasing fresh and dry mass of garden pansy’s flower by 25.10 and 25.41 %, respectively. Also, the content of chlorophyll a increased by 12.93 % under the salinity of 6 dS m-1 with applying 8 mg l-1sodium selenate compared to the case of non-application. The superoxide dismutase activity decreased by 26.13 % compared to the non-sodium selenate usage treatment. In conclusion the foliar application of sodium selenate at the concentraion of 8 mg l-1 resulted in the garden pansy’s growth improvement.


garden pansy; superoxide dismutase; number of flowers; salinity stress; chlorophyll content

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Al Hassan, M, Morosan, M, López-Gresa, MP, Prohens, J, Vicente, O and Boscaiu, M. (2016 a). Salinity-induced variation in biochemical markers provides insight into the mechanisms of salt tolerance in common (Phaseolus vulgaris) and runner (P. coccineus) beans. International Journal of Molecular Science, 17, 1582. https://doi.org/10.3390/ijms17091582

Al Hassan, M, López-Gresa, MP, Boscaiu, M and Vicente, O. (2016 b). Stress tolerance mechanisms in Juncus: responses to salinity and drought in three Juncus species adapted to different natural environments. Functional Plant Biology, 43, 949-960. https://doi.org/10.1071/FP16007

Al Hassan, M, Martínez Fuertes, M, Ramos Sánchez, FJ, Vicente, O and Boscaiu, M. (2015). Effects of salt and water stress on plant growth and on accumulation of osmolytes and antioxidant compounds in cherry tomato. Notulae Botanicae Horti Agrobotanici, 43, 1-11. https://doi.org/10.15835/nbha4319793

Ashraf, M and Harris, PJC. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Science Journal, 166, 3-16. https://doi.org/10.1016/j.plantsci.2003.10.024

Ashraf, M, Ahmad, R, Bhatti, AS, Afzal, M, Sarwar, A, Maqsood, MA, and Kanwal, S. (2010). Amelioration of salt stress in sugarcane (Saccharum officinarum L.) by supplying K and silicon in hydroponics. Pedosphere, 20, 153-162. https://doi.org/10.1016/S1002-0160(10)60003-3

Bayat, H, Alirezaie, M and Neamati, H. (2012). Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis L.) under salinity stress. Journal of Stress Physiology Biochemistry, 8, 258- 267.

Bocchini, M, D’Amato, R, Ciancaleoni, S, Fontanella, MC, Palmerini, CA, Beone, GM and Businelli, D. (2018). Soil Selenium (Se) Biofortification changes the physiological, biochemical and epigenetic responses to water stress in )Zea mays L.( by inducing a higher drought tolerance. Frontier Plant Science, 9, 389. https://doi.org/10.3389/fpls.2018.00389

Bradford, MM. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annal Biochemistry, 72, 248–254. https://doi.org/10.1016/0003-2697(76)90527-3

Cassaniti, C, Romano, D and Flowers, TJ. (2012). The response of ornamental plants to saline irrigation water. In: Irrigation–Water Management, Pollution and Alternative Strategies. I. Garcia-Garizabal (Ed.), IntechOpen, London, UK, 131-158. https://doi.org/10.5772/31787

Bybordi, A. (2016). Effect of zeolite, selenium and silicon on yield, yield components and some physiological traits of canola under salt Stress conditions. Iranian Journal of Field Crop Research, 14, 154-170.

Chu, JZ, Yao, XQ, and Zhang, ZN. (2010). Responses of wheat seedlings to exogenous selenium supply under cold stress. Biological Trace Element Research, 136, 355–363. https://doi.org/10.1007/s12011-009-8542-3

Diao, M, Ma, L, Wangm, J, Cui, J, Fu, A and Liu HY. (2014). Selenium promotes the growth and photosynthesis of tomato seedlings under salt stress by enhancing chloroplast antioxidant defense shoot. Journal of Plant Growth Regulation, 33, 671-682. https://doi.org/10.1007/s00344-014-9416-2

Djanaguiraman, M, Durga Devi, D, Shanker, AK, Sheeba, JA and Bangarusamy U. (2005). Selenium – an antioxidative protectant in soybean during senescence. Plant and Soil, 272, 77-86. https://doi.org/10.1007/s11104-004-4039-1

Feng, R, Wei, C and Tu, S. (2013). The roles of selenium in protecting plants against abiotic stresses. Environmental Experimental Botany, 87, 58-68. https://doi.org/10.1016/j.envexpbot.2012.09.002

Germ, M, Stibilj, V and Kreft, I. (2007). Metabolic importance of selenium for plants. European Journal of Plant Science Biotechnology, 1, 91-97

Giannopolitis, C.N. and Ries, S.K. (1977) Superoxide dismutases. Occurrence in higher plants. Plant Physiology, 59, 309- 314. https://doi.org/10.1104/pp.59.2.309

Gierth, M and Mäser, P. (2007). K transporters in plants−Involvement in K+ acquisition, redistribution and homeostasis. FEBS Letters, 581, 2348- 2356. https://doi.org/10.1016/j.febslet.2007.03.035

Grigore, MN, Boscaiu, M, Llinares, J and Vicente, O. (2012). Mitigation of salt stress-induced Inhibition of Plantago crassifolia reproductive development by supplemental calcium or magnesium. Notulae Botanicae Horti Agrobotanici, 40, 58-66. https://doi.org/10.15835/nbha4028246

Habibi, G. and S. Sarvary. 2015. The Roles of Selenium in Protecting Lemon Balm against Salt Stress. Iranian Journal of Plant Physiology, 5, 1425-1433.

Hasanuzzaman, M, Nahar, K and Fujita, M. (2013). Plant response to salt stress and role of exogenous protectants to mitigate salt induced damages. In: Ecophysiology and responses of plants under salt stress. pp. 25-87. https://doi.org/10.1007/978-1-4614-4747-4_2

Hasanuzzaman, M and Fujita, M. (2011). Selenium pretreatment up regulates the antioxidant defense and methylglyoxal detoxification syshoot and confers enhanced tolerance to drought stress in rapeseed seedlings. Biological Trace Element Research, 143, 1758–1776. https://doi.org/10.1007/s12011-011-8998-9

Hasanuzzaman, M, Anwar Hossain, M, and Fujita, M. (2010). Selenium in higher plants: physiological role, antioxidant metabolism and abiotic stress tolerance. Journal of Plant Science, 5, 354-375. https://doi.org/10.3923/jps.2010.354.375

Hasegawa, P M and Bressan, RA. (2000). Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology, 51, 463-99. https://doi.org/10.1146/annurev.arplant.51.1.463

Hashem, HA, Hassanein RA, Bekheta MA and El-Kady, FA. (2013). Protective role of selenium in canola (Brassica napus L.) plant subjected to salt stress. Egyptian Journal of Experimental Biology, 9, 199-211.

Hawrylak-Nowak, B, Rubinowska, K, Molas, J, Woch, W, Matraszek-Gawron, R and Szczurowska, A. (2019). Selenium-induced improvements in the ornamental value and salt stress resistance of Plectranthus scutellarioides (L.) R. Br. Folia Horticulturae, 31, 213-221. https://doi.org/10.2478/fhort-2019-0016

Hoagland, DR and Arnon, DI. (1950). The Water-Culture Method for Growing Plants without Soil. California Agricultural Experiment Station, Circular-347.

Hu, Y and Schmidhalter, U. (2005). Drought and salinity: a comparison of their effects on mineral nutrition of plants. Soil Science and Plant Nutrition, 168, 541-549. https://doi.org/10.1002/jpln.200420516

Kaur, N., Sharma, S., Kaur, S., Nayyar, H., (20140. Selenium in agriculture: a nutrient or contaminant for crops? Archives of Agronomy and Soil Science, 60, 1593-1624. https://doi.org/10.1080/03650340.2014.918258

Khan, MS, Ahmad, D and Khan, MA. (2015). Trends in genetic engineering of plants with (Na+/H+) antiporters for salt stress tolerance. Biotechnology Biotechnological Equipment, 29, 815–825. https://doi.org/10.1080/13102818.2015.1060868

Kozminska, A, Al Hassan, M, Kumar, D, Oprica, L, Martinelli, F, Grigore, MN, Vicente, O and Boscaiu, M. (2017). Characterizing the effects of salt stress in Calendula officinalis L. Journal of Applied Bot any and Food Quality, 90, 323 - 329

Kumar, D, Al Hassan, M, Naranjo, MA, Agraval, V, Boscaiu, M and Vicente, O. (2017). Effects of salinity and drought on growth, ionic relations, compatible solutes and activation of antioxidant shoots in oleander (Nerium oleander L.). PLoS ONE, 12, 1-22. https://doi.org/10.1371/journal.pone.0185017

Liang, Y, Zhang, W, Chen, Q, Liu, Y and Ding, R. (2006). Effect of exogenous silicon (Si) on H+-ATPase activity, phospholipids and fluidity of plasma membrane in leaves of salt-stressed barley (Hordeum vulgare L.). Environmental Experimental Botany, 57, 212-219. https://doi.org/10.1016/j.envexpbot.2005.05.012

Lichtenthaler, HK and Wellburn, AR. (1983). Determination of total carotenoids and chlorophyll a and b of leaf extracts in different solvents. Biochemical Society Transactions, 603, 591–592. https://doi.org/10.1042/bst0110591

Liu, KL and Gu, ZX. (2009). Selenium accumulation in different brown rice cultivars and its distribution in fractions. Journal of Agricultural and Food Chemistry, 57, 695-700. https://doi.org/10.1021/jf802948k

Mahajan, S and Tuteja, N. (2005). Cold, salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics, 444, 139-158. https://doi.org/10.1016/j.abb.2005.10.018

Malash, NM, Flowers TJ and Ragab, R. (2008). Effect of irrigation methods, management and salinity of irrigation water on tomato yield, soil moisture and salinity distribution. Irrigation Science, 26, 313-323. https://doi.org/10.1007/s00271-007-0095-7

Malik, JA, Goel, S, Kaur, N, Sharma, S, Singh, I and Nayyar, H. (2012). Selenium antag-onises the toxic effects of arsenic on mungbean (Phaseolus aureus Roxb.) plants by restricting its uptake and enhancing the antioxidative and detoxification mechanisms. Environmental Experimental Botany, 77, 242–248. https://doi.org/10.1016/j.envexpbot.2011.12.001

Matraszek, R, Hawrylak-Nowak, B and Chwil, M. (2015). Protein hydrolysate as a component of salinized soil in the cultivation of Ageratum houstonianum Mill. (Asteraceae). Acta Agrobotics, 68, 247-253. https://doi.org/10.5586/aa.2015.028

Matos, RP, Lima, VM, Windmüller, CC and Nascentes, CC. (2017). Correlation between the natural levels of selenium and soil physicochemical characteristics from the Jequitinhonha Valley (MG). Brazilian Journal of Geochemistry Exploration, 172, 195-202. https://doi.org/10.1016/j.gexplo.2016.11.001

Mirlotfi, A, Bakhtiari, S and Bazrgar, AB. (2015). Effect of seed priming on germination and seedling traits of Marigold (Calendula officinalis) at saline condition. Biological Forum and International Journal, 7, 1626-1630.

Munshower, FF. (2018). Practical Handbook of Disturbed Land Revegetation: 0. CRC Press. https://doi.org/10.1201/9781351075923

Munns, R and Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651-681. https://doi.org/10.1146/annurev.arplant.59.032607.092911

Nofal, FH, El-Segai, MU and Seleem, EA. (2015). Response of Calendula officinalis L. plants to growth stimulants under salinity stress. Journal of Applied Engineering Science, 15, 1767-1778.

Parihar, P, Singh, S, Singh, R, Singh VJ, Prasad and SM. (2015). Effect of salinity stress on plants and its tolerance strategies: a review. Environmetal Science and Pollution Research, 22, 4056-4075. https://doi.org/10.1007/s11356-014-3739-1

Pezzarossa, B, Remorini, D, Gentile, ML and Massai, R. (2012). Effects of foliar and fruit addition of sodium selenate on selenium accumulation and fruit quality. Journal of Scientific Food Agriculture, 92, 781–786. https://doi.org/10.1002/jsfa.4644

Pazurkiewicz-Kocot, K, Kita, A and Pietruszka, M. (2008). Effect of selenium on magnesium, iron, manganese, copper, and zinc accumulation in corn treatedbyindole-3-aceticacid. Communications in Soil. Science and Plant Analysis, 39, 2303–2318. https://doi.org/10.1080/00103620802292343

Rawson, HM, Iong, MJ and Munns, R. (1988). Growth and development in NaCl treated plants. Journal of Plant Physiology, 15, 519-527. https://doi.org/10.1071/PP9880519

Rios, JJ, Blasco, B, Cervilla, LM, Rosales, MA, Sanchez-Rodriguez, E, Romero, L and Ruiz, JM. (2009). Production and detoxification of H2O2 in lettuce plants exposed to selenium. Annals Applied Biology, 154, 107-116. https://doi.org/10.1111/j.1744-7348.2008.00276.x

Rodríguez-Navarro, A. (2000). K transport in fungi and plants. Biochimia et Biophysica Acta, 1469, 1-30. https://doi.org/10.1016/S0304-4157(99)00013-1

Satyendra, NR, Stephan, WB, Gossett, DR, Lucas and MC. (1999). Antioxidant response to salt stress during fiber development in cotton ovules. Journal of Cotton Science, 30, 11-15.

Shahid, M, Niazi, NK, Khalid, S, Murtaza, B, Bibi, I, Rashid, MI. (2018). A critical review of selenium biogeochemical behavior in soil-plant system with an inference to human health. Environmetal Pollution, 234, 915-934. https://doi.org/10.1016/j.envpol.2017.12.019

Shahzadi, I, Iqbal, M, Rasheed, R, Arslan Ashraf, M, Perveen, S and Hussain, M. (2017). Foliar application of selenium increases fertility and grain yield in bread wheat under contrasting water availability regimes. Acta Physiologiae Plantarum, 39, 173. https://doi.org/10.1007/s11738-017-2477-7

Sharma, PR and Dubey, S. (2005). Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant growth regulation, 46, 209-221. https://doi.org/10.1007/s10725-005-0002-2

Supriatin, S, Weng, L and Comans, RN. (2015). Selenium speciation and extractability in Dutch agricultural soils. Science of the Total Environment, 532, 368-382. https://doi.org/10.1016/j.scitotenv.2015.06.005

Tan, LC, Nancharaiah, YV, van Hullebusch, ED and Lens, PN. (2018) Selenium: environmental significance, pollution, and biological treatment technologies. In: Anaerobic Treatment of Mine Wastewater for the Removal of Selenate and its Co-Contaminants, (pp. 9-71). CRC Press. https://doi.org/10.1201/9780429448676-2

Turakainen, M. (2007). Selenium and its effects on growth, yield and tuber quality in potato. University of Helsinki, Helsinki (Doctor thesis).

Vukics, V, Kery, A, and Guttman, A. (2008). Analysis of polar antioxidants in heartsease (Viola tricolor L.) and garden pansy (Viola x wittrockiana Gams.). Journal of Caring Sciences, 46, 823-827. https://doi.org/10.1093/chromsci/46.9.823

Wang, CQ. (2011). Water-stress mitigation by selenium in Trifolium repens L. Journal of Soil Science and Plant Nutrition, 174, 276-282. https://doi.org/10.1002/jpln.200900011

Wang, J, Wang, Z, Mao, H, Zhao, H and Huang, D. (2013). Increasing Se concentration in maize grain with soil- or foliar-applied selenate on the Loess Plateau in China. Field Crops Research, 150, 83–90. https://doi.org/10.1016/j.fcr.2013.06.010

Yao, X, Chu, J, He, X, and Ba, C. (2011). Protective role of selenium in wheat seedlings subjected to enhanced UV-B radiation. Russain Journal of Plant Physiology, 58, 283-289. https://doi.org/10.1134/S1021443711020257

Zhu, Z., Chen, Y., Zhang, X., Li, M., 2016. Effect of foliar treatment of sodium selenate on postharvest decay andquality of tomato fruits. Scientia Horticulturae, 198, 304–310. https://doi.org/10.1016/j.scienta.2015.12.002

DOI: http://dx.doi.org/10.14720/aas.2020.115.2.1475


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