Parasitoid inventarisation of European corn borer (Ostrinia nubilalis Hübner, 1796) and options for its biological control in Slovenia

Jaka RAZINGER, Špela MODIC, Annette HERZ, Gregor UREK


European corn borer (Ostrinia nubilalis) (ECB) is an important maize pest in central and northern Europe. Presently it is controlled by insecticides or biological agents such as Trichogramma brassicae in several European countries, excluding Slovenia, where the pest’s pressure is highly variable and no appropriate mechanization is available. Lessening the dependence on chemical pesticides is an integral part of the European Union’s agenda for agriculture. Mass release of Trichogramma spp. could be seen as a promising alternative for ECB control in countries with a highly fluctuating ECB pressure and no mechanization for insecticide applications. However, no records of naturally occurring hymenopteran parasitoids of ECB exist in Slovenia. To address this important under-researched topic and provide the expert basis for potential introduction of ECB egg parasitoids in Slovene maize production, a systematic inventarisation programme of ECB parasitoids was launched in 2010. Additionally, ECB flight was monitored in 2011 and 2012 at two locations in Slovenia: Jablje and Rakičan. In both locations two ECB generations  were observed. ECB was fist observed at the end of May in Rakičan. During the five years of the systematic survey we discovered two ECB parasitoid species. ECB egg masses were parasitized by Trichogramma brassicae, whereas ECB pupae were parasitized by Tycherus nigridens, with 6 or 7 % parasitation rate, respectively. T. nigridens represents a new taxon report for Slovenia. We conclude that there is a strong need for undertaking systematic surveys of natural enemies of agricultural pests.


biological control; corn; Ichneumonidae; insect pests; maize; new records; parasitoids; Tycherus nigridens; Trichogrammatidae; Trichogramma brassicae; Zea mays

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Babendreier D, Kuske S, Bigler F, 2003a. Non-target host acceptance and parasitism by Trichogramma brassicae

Bezdenko (Hymenoptera: Trichogrammatidae) in the laboratory. Biological Control 26, 128-138. DOI: 10.1016/S1049-9644(02)00121-4

Babendreier D, Kuske S, Bigler F, 2003b. Parasitism of non-target butterflies by Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae) under field cage and field conditions. Biological Control 26, 139-145. DOI: 10.1016/S1049-9644(02)00120-2.

Baker W.A., Bradley W.G., Clark C.A., 1949. Biological Control of the European Corn Borer in the United States. Technical Bulletin 983, 185 pp.

Bohinc T., Schmidt S., Monje J. C., Trdan S., 2015. First record of parasitic wasp Trichogramma brassicae Bezdenko, 1968 (Hymenoptera, Trichogrammatidae) in Slovenia. Acta agriculturae Slovenica 105, 323 – 327. DOI: 10.14720/aas.2015.105.2.15

Boivin G., 2010, Reproduction and Immature Development of Egg Parasitoids. In: Cônsoli, Parra, Zucchi (Eds.), 2010. Egg Parasitoids in Agroecosystems with Emphasis on Trichogramma. Springer Dordrecht. ISBN 978-1-4020-9109-4, 1-23.

Brockerhoff E.G., Kenis M., 1996. Parasitoids Associated with Cydia strobilella (L.) (Lepidoptera: Tortricidae) in Europe, and Considerations for Their Use for Biological Control in North America. Biological Control 6, 202-214. DOI: 10.1006/bcon.1996.0025

Butrón A., Revilla P., Sandoya G., Ordás A., Malvar R.A., 2009. Resistance to reduce corn borer damage in maize for bread, in Spain. Crop Protection 28, 134-138. DOI: 10.1016/j.cropro.2008.09.007

Carlevaris B., Gomboc S., Milevoj L., 2003. Study on European corn borer (Ostrinia nubilalis Hbn.) on different corn hybrids in Goriška region. Zbornik predavanj in referatov 6. slovenskega posvetovanja o varstvu rastlin, Zreče, 4. – 6. marec 2003, 176-182.

Chapman A.V., Kuhar T.P., Schultz P.B., et al., 2009. Integrating Chemical and Biological Control of European Corn Borer in Bell Pepper. Journal of Economic Entomology 102, 287-295. DOI: 10.1603/029.102.0138

Cônsoli F.L., Parra J.R.P., Zucchi R.A., 2010. Egg Parasitoids in Agroecosystems with Emphasis on Trichogramma. Springer Dordrecht Heidelberg London New York. ISBN 978-1-4020-9109-4, DOI 10.1007/978-1-4020-9110-0, 479 pp.

European Parliament, 2009. Directive 2009/128/EC of the European Parliament and of the Council.

Ferriere Ch., Kerrich G.J., 1958. Handbooks for the identification of British insects. Hymenoptera, 2. Chalcidoidea, section (a). Vol. VIII. Part 2 (a). Royal Entomological Society, London, 44 pp.

Gomboc S., Carlevaris B., Vrhovnik D., Milevoj L., Celar F., 1999. Bionomy of European corn borer (Ostrinia nubilalis Hb.) in Slovenia. Lectures and papers presented at the 4th Slovenian conference on plant protection in Portorož, March 3.-4. Plant Protection Society of Slovenia, Ljubljana, Slovenia, 1999. 459-467.

Goulet H., Huber J.T., 1993. Hymenoptera of the world: An identification guide to families. Agriculture Canada, Ottawa, Canada. ISBN 0-660-14933-8, 680 pp.

Herz A, Hassan S.A., Hegazi E., Khafagi W.E., Nasr F.N., Youssef A.I., Agamy E., Blibech I, Ksentini I., Ksantini M., Jardak T., Bento A., Pereira J.A., Torres L., Souliotis C., Moschos T., Milonas P., 2007. Egg parasitoids of the genus Trichogramma (Hymenoptera, Trichogrammatidae) in olive groves of the Mediterranean region. Biological Control 40, 48-56. DOI: 10.1016/j.biocontrol.2006.08.002

Hoffmann M.P., Pitcher S.A., Cheever S.A., Gardner J., Losey J.E., Kuhar T.P., Laub C.A., Youngman R.R., 2006. Efficacy of inoculative releases of Trichogramma ostriniae (Hymenoptera: Trichogrammatidae) against European corn borer Ostrinia nubilalis (Lepidoptera: Crambidae) in field corn. Biological Control 36, 345-349. DOI: 10.1016/j.biocontrol.2005.10.008

Hoffmann M.P., Wright M.G., Pitcher S.A., Garder J., 2002. Inoculative releases of Trichogramma ostriniae for suppression of Ostrinia nubilalis (European corn borer) in sweet corn: field biology and population dynamics. Biological Control 25, 249-258. DOI: 10.1016/S1049-9644(02)00105-6

Jenner, W.H.; Kuhlmann, U.; Cossentine, J.E.; Roitberg, B.D., 2004. Phenology, distribution, and the natural parasitoid community of the cherry bark tortrix. Biological Control 31, 72-82. DOI: 10.1016/j.biocontrol.2004.05.007

Malvar R.A., Butrón A., Álvarez A., Ordás B., Soengas P., Revilla P., Ordás A., 2004. Evaluation of the European Union maize landrace core collection for resistance to Sesamia nonagrioides (Lepidoptera: Noctuidae) and Ostrinia nubilalis (Lepidoptera: Crambidae). J. Econ. Entomol. 97, 628–634. DOI: 10.1603/0022-0493-97.2.628

Meissle M., Mouron P., Musa T., Bigler F., Pons X., Vasileiadis V.P., Otto S., Antichi D., Kiss J., Palinkas Z., Dorner Z., van der Weide R., Groten J., Czembor E., Adamczyk J., Thibord J.-B., Melander B., Cordsen Nielsen G., Poulsen R.T., Zimmermann O., Verschwele A., Oldenburg E., 2010. Pests, pesticide use and alternative options in European maize production: current status and future prospects. J. Appl. Entomol. 134, 357–375. DOI: 10.1111/j.1439-0418.2009.01491.x

Pinto J.D., 2006. A review of the New World genera of Trichogrammatidae (Hymenoptera). Journal of Hymenoptera Research 15 (1), 38-163.

Official Gazette of the Republic of Slovenia: Plant Protection Products Act (UL RS 83/12); Plant Protection Act (UL RS 62/07, 36/10 and 40/14); Rules on the Biological Plant Protection (UL RS 45/06).

Pintureau B., 1987. Systématic évolutive du genre Trichogramma Westwood in Europe. Thesis, Université Paris VII. 311 pp.

Pintureau B., 2011. Les especes europeennes de Trichogrammes (French Edition). InLibroVeritas, France. ISBN 978-2-35209-181-3, 96 pp.

Pintureau B., Voegelé J., 1980: Une nouvelle espèce proche de Trichogramma evanescens: T. maidis (Hym.: Trichogrammatidae). Entomophaga 25, 431-440. DOI: 10.1007/BF02374706

Platner G.R., Velten R.K., Planoutene M., Pinto J.D., 1999: Slide mounting techniques for Trichogramma (Trichogrammatidae) and other minute parasitic Hymenoptera. Ent. News 110 (1), 56-64.

Pons X., Albajes R., 2002. Control of maize pests with imidacloprid seed dressing treatment in Catalonia (NE Iberian Peninsula) under traditional crop conditions. Crop Protection 21, 943-950. DOI: 10.1016/S0261-2194(02)00069-8

Querino R.B., Roberto A.Z., Pinto J.D., 2010. Systematics of the Trichogrammatidae (Hymenoptera: Chalcidoidea) with a Focus on the Genera Attacking Lepidoptera. In: Cônsoli, Parra, Zucchi (Eds.), 2010. Egg Parasitoids in Agroecosystems with Emphasis on Trichogramma. Springer Dordrecht. ISBN 978-1-4020-9109-4, 191-218.

Smith H.D., 1932. Phaeogenes nigridens Wesmael, an Important Ichneumonid Parasite of the Pupa of the European Corn Borer. Washington DC, United States Department of Agriculture, Technical Bulletin 331, 46 pp.

Sobek E.A., Munkvold G.P., 1999. European corn borer (Lepidoptera: Pyralidae) larvae as vectors of Fusarium moniliforme, causing kernel rot and symptom less infection of maize kernels. J. Econ. Entomol. 92, 503–509. DOI: 10.1093/jee/92.3.503

Stengel M., Voegelé J., Lewis J.W., 1977. Les Trichogrammes. V.b. Survie hivernale de Trichogramma evanscens Westw. souche moldave et découverte de T. cacoeciae Mar. sur pontes d’ Ostrinia nubilalis. Ann. Zool. Ecol. Anim. 9 (2), 313-317.

Velasco P., Revilla P., Butrón B., Ordás B., Ordás A., Malvar R.A., 2002. Ear damage of sweet corn inbreds and their hybrids under multiple corn borer infestation. Crop Sci. 42, 724–729. DOI: 10.2135/cropsci2002.0724

Zhang F., Babendreier D., Wang Z.–Y, Il K.S., Zheng L., Pyon Y.C., Bai S.-X., Song K., Ri J.O., Grossrieder M., Kuhlmann U., 2010. Mass releases of Trichogramma ostriniae increase maize production in DPR Korea. Journal Of Applied Entomology 134, 481-490. DOI: 10.1111/j.1439-0418.2010.01512.x



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