Variation in antioxidant, and antibacterial activities and total phenolic content of the bulbs of mooseer (Allium hirtifolium Boiss.)



Allium hirtifolium Boiss. (mooseer) belonging to the family Alliaceae, is an endemic species of Iran which grows wild in the Zagros Mountains range, western and southwestern Iran. The bulb of A. hirtifolium has been used as a flavouring agent, especially dairy foods and pickles by the indigenous people, southwestern Iran. In this study, the bulbs of various populations of the plant were collected from the alpine regions in Chaharmahal va Bakhtiari province, Iran. The total phenolic content of the ethanol extract was determined by Folin– Ciocalteu method, the antioxidant activity was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and the antibacterial activity of the extracts against four bacteria, including Bacillus cereus, Listeria monocytogenes, Proteus vulgaris, and Salmonella typhimurium was determined by serial dilution assay. Results indicated that the total phenolic content in the ethanol extracts of different populations of A. hirtifolium ranged between 34 to 44 mg gallic acid/g extract. In addition, the extracts of A. hirtifolium indicated moderate– to–good inhibitory activities (MICs = 0.062 to 0.250 mg/ml) against four bacteria, especially against B. cereus. The antioxidant activity of the bulbs of A. hirtifolium indicated the extract acted as an effective DPPH scavenger, but were not as effective as the BHT control. This finding suggests that the bulbs of A. hirtifolium may be considered as a natural source of antioxidants and antimicrobial agents.


allium; antioxidants; bulbs; antibacterial properties; antimicrobial properties; antimicrobials; phenolic compounds; phenolic content; extracts; indigenous organisms

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Ahmad, I., Beg, A.Z. 2001. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Journal of Ethnopharmacology, 74: 113–123; DOI: 10.1016/S0378-8741(00)00335-4

Amin, G.R. 1991. Popular medicinal plants of Iran, Tehran: Iranian Ministry of Health publication, p. 37.

Ariga, T., Seki, T. 2006. Antithrombotic and anticancer effects of garlic-derived sulfur compounds: a review, BioFactors, 26: 93–103; DOI: 10.1002/biof.5520260201

Ashrafi, F., Akhavan Sepahi, A., Kazemzadeh, A. 2004. Effect of aqueous extract of shallot (Allium ascalonicum) on inhibition of growth of Pseudomonas aeroginosa. Iranian Journal of Pharmaceutical Research, 2: 71–71.

Benkeblia, N. 2005. Free-radical scavenging capacity and antioxidant properties of some selected onions (Allium cepa L.) and garlic (Allium sativum L.) extracts. Brazilian Archives of Biology and Technology, 48: 753–759; DOI: 10.1590/S1516- 89132005000600011

CLSI (Clinical and Laboratory Standards Institute). 2012. Performance standards for antimicrobial disks susceptibility tests: approved standards - Eleventh edition. M02-A11, CLSI, USA.

Corzo-Martinez, M., Corzo, N., Villamiel, M. 2007. Biological properties of onion and garlic. Trends in Food Science Technology, 18: 609–625; DOI: 10.1016/j.tifs.2007.07.011

Fritsch, R.M., Keusgen, M. 2006. Occurrence and taxonomic significance of cysteine sulphoxides in the genus Allium L. (Alliaceae). Phytochemistry, 67: 1127–1135; DOI: 10.1016/j.phytochem.2006.03.006

Ghahremani-majd, H., Dashti, F., Dastan, D., Mumivand, H., Hadian, J., Esna-Ashari, M. 2012. Antioxidant and antimicrobial activities of Iranian mooseer (Allium hirtifolium Boiss) populations. Horticulture, Environment, and Biotechnology, 53: 116–122; DOI: 10.1007/s13580-012-0131-2

Ghasemi Pirbalouti, A. 2009. Medicinal plants used in Chaharmahal and Bakhtyari districts, Iran. Herba Polonica, 55: 69–75.

Ghasemi Pirbalouti, A., Setayesh, M., Siahpoosh, A., Mashayekhi, H. 2013a. Antioxidant activity, total phenolic and flavonoids contents of three herbs used as condiments and additives in pickles products. Herba Polonica, 59: 51–62.

Ghasemi Pirbalouti, A., Fatahi-Vanani, M., Craker, L., Shirmardi, H. 2013b. Chemical composition and bioactivity of essential oils of Hypericum helianthemoides. Hypericum perforatum and Hypericum scabrum. Pharmaceutical Biology, 52(2): 175-181; DOI: 10.3109/13880209.2013.821663

Ghahreman, A., 1984. Color Atlas of Iranian Plants. Institute of Forestries and Grasslands, Botany Division, No. 5, 512 pp.

Ghodrati Azadi, H., Ghaffari, S.M., Riazi, G.H., Ahmadian, S., Vahedi, F. 2008. Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor cell lines. Cytotechnology, 56: 179–185; DOI: 10.1007/s10616-008-9145-0

Hung, D., Ou, B., Prior, R.L. 2005. The chemistry behind antioxidant capacity assay. J. Journal of Agricultural and Food Chemistry, 53: 1841–1856; DOI: 10.1021/jf030723c

Jafarian, A., Ghannadi, A., Elyasi, A. 2003. The effects of Allium hirtifolium Boiss on cell-mediated immune response in mice. Iranian Journal of Pharmaceutical Research, 2: 51–55.

Jaffery, E.H., Brown, A.F., Kurilich, A.C., Keek, A.S., Matusheski, N., Klein, B.P. 2003. Variation in content of bioactive components in broccoli. Journal of Food Composition and Analysis, 16: 323–330; DOI: 10.1016/S0889-1575(03)00045-0

Ismail, S., Jalilian, F. A., Talebpour, A. H., Zargar, M., Shameli, K., Sekawi, Z., Jahanshiri, F. 2013. Chemical composition and antibacterial and cytotoxic activities of Allium hirtifolium boiss. BioMed Research International, DOI: 10.1155/2013/ 696835.

Kazemi, S., Asgary, S., Moshtaghian, J., Rafieian, M., Adelnia, A., Shamsi, F. 2010. Liver-protective effects of hydroalcoholic extract of Allium hirtifolium boiss. In rats with alloxan-induced diabetes mellitus. ARYA Atheroscler, 6: 11–15.

Kojuri, J., Vosoughi, A. R., Akrami, M. 2007. Effects of Anethum graveolens and garlic on lipid profile in hyperlipidemic patients. Lipids Health Disease, 6: 1- 5; DOI: 10.1186/1476-511X-6-5

Mozaffarian, V. 2008. A pictorial dictionary of medicinal plants. Latin–English–French–Germany– Persian/Complied, Farahang Moaser, Tehran, pp. 522.

Najjaa, H., Ammar, E., Neffati, M. 2009. Antimicrobial activities of protenic extracts of Allium roseum L., a wild edible species in North Africa. Journal of Food Agriculture and Environment, 7: 150–154.

Prakash, D., Singh, B. N., Upadhyay, G. 2007. Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa). Food Chemistry 102(4): 1389–1393; DOI: 10.1016/j.foodchem.2006.06.063

Rechinger, K.H. 1984. Flora Iranica, Alliaceae, vol. 76. Akademische Druck, Univ. Verlagsanstalt Graz, Austria, 85 pp.

Rodriguez, H., Curiel, J.A., Landete, J.M., De las Rivas, B., De Felipe, F.L., Gomez- Cordoves, C., Mancheno, J.M., Munoz, R. 2009. Food phenolics and lactic acid bacteria. International Journal of Food Microbiology, 132: 79–90; DOI: 10.1016/j.ijfoodmicro.2009.03.025

Singleton, V.L., Rossi, J.A. 1965. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. American Society for Enology and Viticulture, 16: 144–158.

Taguri, T., Tanaka, T., Kouno, I. 2006. Antibacterial spectrum of plant polyphenols and extracts depending upon hydroxyphenyl structure. Biological & Pharmaceutical Bulletin, 29: 2226– 2235; DOI: 10.1248/bpb.29.2226

Tepe, B., Sokmen, M., Akpulat, H.A., Sokmen, A. 2005. In vitro antioxidant activities of the methanol extracts of five Allium species from Turkey. Food Chemistry, 92: 89–92; DOI: 10.1016/j.foodchem.2004.07.016

Thormar H. 2011. Lipids and essential oils as antimicrobial agents. John Wiley & Sons, Ltd, Chichester, UK. DOI: 10.1002/9780470976623.

Velioglu, Y. S., Mazza, G., Gao, L., Oomah, B.D. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. Journal of Agricultural and Food Chemistry, 46: 4113–4117; DOI: 10.1021/jf9801973



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