Email this article Effect of plant growth regulators on the growth and direct shoot formation from leaf explants of the hybrid Phalaenopsis ‘Pink’
Abstract
Phalaenopsis orchids are one of the most beautiful flowering plants. The objective of this study was to identify the best plant growth regulator combination and medium for the growth and direct shoot formation from leaf explants of the hybrid Phalaenopsis ‘Pink’. Leaf tips segments from in vitro young plants were cultured on half-strength Murashige and Skoog (MS) and Vacin and Went (VW) media supplemented with different concentrations of auxin [α-napthaleneacetic acid (NAA)] and cytokinins [6-benzylaminopurine (BAP) or thidiazuron (TDZ)]. The explants that were cultured on 0 mg l–1 NAA and 3 mg l–1 BAP supplemented to half-strength MS medium formed shoots successfully within 10 weeks of culture with 5 % regenerants and 50 % survival frequency. The explants cultured on 0.5 mg l–1 NAA and 1.5 mg l–1 TDZ supplemented to half-strength MS medium developed calluses and shoots within 11 weeks of culture with 25 % regenerants and 90 % survival frequency. Future research needs to be directed to find out the shortest time of shoot regeneration to produce viable plants with a high survival frequency.
Keywords
Full Text:
PDFReferences
Arditti J.R., Ernst. (1993). Micropropagation of Orchids. Wiley-Interscience, New York, USA.
Chen Y.C., Chang C., Chang W.C. (2000). A reliable protocol for plant regeneration from callus culture of Phalaenopsis. In Vitro Cellular & Developmental Biology – Plant, 36, 420–423. doi:10.1007/s11627-000-0076-5
Chen J.T., Chang W.C. (2001). Effects of auxins and cytokinin on direct somatic embryogenesis from leaf explants of Oncidium ‘Grower Ramsey’. Plant Growth Regulation, 34, 229–232. doi:10.1023/A:1013304101647
Chen J.T., Chang W.C. (2004). Induction of repetitive embryogenesis from seed derived protocorms of Phalaenopsis amabilis ‘Formosa Shimadzu’. In Vitro Cellular & Developmental Biology – Plant, 40, 209–293. doi:10.1079/IVP2003527
Gaspar T.H., Kevers C., Faivre-rampant O., Crevecoeur M., Penel C., Greppin H., Dommes J. (2003). Changing concepts in plant hormones action. In Vitro Cellular & Developmental Biology – Plant, 39, 85–106. doi:10.1079/IVP2002393
George F.E., Sherrington P.D. (1984). Plant Propagation by Tissue Culture: Handbook and directory of commercial laboratories. Exegetics Ltd., Edington, England, UK, 1984. pp. 709.
Ishii Y., Takamura T., Goi M., Tanaka M. (1998). Callus induction and somatic embryogenesis of Phalaenopsis. Plant Cell Reports, 17, 446–450. doi:10.1007/s002990050423
Jimenez V.M. (2005). Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regulation, 47, 91–110. doi:10.1007/s10725-005-3478-x
Košir P., Škof S., Luthar Z. (2004). Direct shoot regeneration from nodes of Phalaenopsis orchids. Acta Agriculturae Slovenica, 83, 233–242.
Koh W.L., Loh C.S. (2000). Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid cycling Brassica napus. Plant Cell Reports, 19, 1177–1183. doi:10.1007/s002990000268
Kuo H.L., Chen J.T., Chang W.C. (2005). Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis ‘Little Steve’. In Vitro Cellular & Developmental Biology – Plant, 41, 453–456. doi:10.1079/IVP2005644
Lu C.Y. (1993). The use of thidiazuron in tissue culture. In Vitro Cellular & Developmental Biology – Plant, 29, 92–96. doi:10.1007/BF02632259
Moharami L., Hosseini B., Ravandi E.G., Jafari M. (2014). Effects of plant growth regulators and explant types on in vitro direct plant regeneration of Agastache foeniculum, an important medicinal plant. In Vitro Cellular & Developmental Biology – Plant, 50, 707–711. doi:10.1007/s11627-014-9640-2
Mok M.C., Mok D.W.S., Armstrong D.J., Shudo K., Isogai Y., Okamoto T. (1982). Cytokinin activity of N-Phenyl-N’- (1,2,3-thidiazuron-5-yl) urea (thidiazuron). Phytochemistry, 21, 1509–1511. doi:10.1016/S0031-9422(82)85007-3
Murashige T., Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15, 473–497. doi:10.1111/j.1399-3054.1962.tb08052.x
Niknejad A., Kadir M.A., Kadzimin B.S. (2011). In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroidaceae: Orchidaceae). African Journal of Biotechnology, 10, 11808–11816.
Park S.Y., Murthy H.N., Paek K.Y. (2001). Rapid propagation of Phalaenopsis from floral stalk-derived leaves. In Vitro Cellular & Developmental Biology – Plant, 38, 162–172.
Purohit S.S. (2005). Plant tissue culture. Student Edition, Singapore. pp. 100–125.
Teob E.S. (1989). Orchid of Asia. Times Books International, Singapore.
Tokuhara K., Mii M. (1993). Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Reports, 13, 7–11. doi:10.1007/BF00232306
Tokuhara K., Mii M. (2001). Induction of embryonic callus and cell suspension culture from shoot tips excised from flower stalk Buds of Phalaenopsis (Orchidaceae). In Vitro Cellular & Developmental Biology – Plant, 37, 457–461. doi:10.1007/s11627-001-0080-4
Vacin E., Went F.W. (1949). Some pH changes in nutrient solutions. Botanical Gazette, 110, 605–613. doi:10.1086/335561
Zahara M., Datta A., Boonkorkaew P. (2016). Effects of sucrose, carrot juice and culture media on growth and net CO2 exchange rate in Phalaenopsis hybrid ‘Pink’. Scientia Horticulturae, 205, 17–24. doi:10.1016/j.scienta.2016.04.011
DOI: http://dx.doi.org/10.14720/aas.2018.111.1.01
Refbacks
- There are currently no refbacks.
Copyright (c) 2018 Avishek Datta
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Acta agriculturae Slovenica is an Open Access journal published under the terms of the Creative Commons CC BY License.
eISSN 1854-1941