Successful disinfection protocol for orchid seeds and influence of gelling agent on germination and growth

Tomaž JEVŠNIK, Zlata LUTHAR

Abstract


Artificial propagation of endangered orchid species is one of the most important actions of conservationists often jeopardized by low numbers of acquired seed, its contamination and viability. Disinfection and chemical composition of media are two of the most important factors contributing to better germination in temperate orchid species. The article deals with three world genera (Epidendrum nocturnum, Prosthechea garciana, Maxillaria rufescens) and one commercial hybrid (Zygopetalum) and describes an effective method of orchid seed disinfection carried out in a centrifuge. Germination percentages of all three genera and one hybrid were between 60 and 90 % from which we concluded that the risk of physical damage to the seeds by centrifugation is not significant. The time needed for disinfected seeds (E. nocturnum, P. garciana, M. rufescens) to swell-form protocorms was 10 days shorter compared to undisinfected seeds (Zygopetalum hybrid - green capsule method) and some other studies. Adequate wetting and stratification of the seed is very important for successful germination, which resembles processes in natural environment. Additionally, this method solves the problems of collecting and transferring the seeds after disinfection. It is also important that the time needed for disinfection is shorter, which is desirable for some sensitive species. Our study also focuses on importance of gelling agent, namely Gellan gum and agar, since we noticed an obvious superiority of the former in all phases of in vitro development.


Keywords


orchidaceae; endangered species; seeds; plant propagation; germinability; site factors; disinfection; chemicophysical properties; growing media

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References


Arditti J. 1982. Orchid seed germination and seedling culture. A manual. In: Orchid Biology: Reviews and Perspectives, Vol. 2. Arditti, J (ed.). New York: Cornell University Press: 243–293

Arditti J. 1992. Fundamentals of Orchid Biology. New York, John Wiley & Sons: 712 p.

Arditti J., Ghani A.K.A. 2000. Numerical and physical properties of orchid seed and their biological implications. Tansley Review, 145: 367-421

Bhattacharjee S., Khan H.A., Reddy P.V. 1999a. In Vitro Germination of Phalaenopsis Hybrid Seed. Seed Research, 27: 11-13

Bhattacharjee S., Khan H.A., Reddy P.V. 1999b. Effect of Sucrose Levels on in vitro Seed Germination of Phalaenopsis Hybrid. Journal of Hill Research, 12: 58-60

De Pauw M. A., Remphrey W.R. 1993. In vitro germination of three Cypripedium species in relation to time of seed collection, media, and cold treatment. Canadian Journal of Botany, 71: 879-885; DOI: 10.1139/b93-100

Gamborg O.L., Murashige T., Thorpe T.A., Vasil I.K. 1976. Plant tissue culture media. In Vitro, 12: 473- 478; DOI: 10.1007/BF02796489

Hicks A.J. 2000. Asymbiotic Technique of Orchid Seed Germination. Chandler, The Orchid Seedbank Project: 134 p.

Hinnen M.G.J., Pierik R.L.M., Bronsema F.B.F. 1989. The Influence of Macronutrients and Some Other Factors on Growth of Phalaenopsis Hybrid Seedlings In Vitro. Scientia Horticulturae, 41: 105-116; DOI: 10.1016/0304-4238(89)90054-X

Johnson T.R., Stewart S.L., Dutra D., Kane M.E., Richardson L. 2007. Asymbiotic and symbiotic seed germination of Eulophia alta (Orchidaceae) preliminary evidence for the symbiotic culture advantage. Plant Cell Tiss. Org. Cult., 90: 313-323; DOI: 10.1007/s11240-007-9270-z

Knudson, L. 1922. Non-symbiotic Germination of Orchid seed. Bot. Gaz., 73: 1-25; DOI: 10.1086/332956 Koopowitz H. 2001. Orchids and their Conservation. London, B.T. Batsfort Ltd.: 176 p.

Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15: 473-497; DOI: 10.1111/j.1399-3054.1962.tb08052.x

Nikishina T.V., Popov A.S., Kolomeitseva G.L., Golovkin B.N. 2001. Effect of cryoconservation of seed germination of rare tropical orchids. Russian Journal of Plant Physiology, 48 (6): 810-815; DOI: 10.1023/A:1012520927743

Pritchard H.W, Seaton P.T. 1993. Orchid seed storage: Historical perspective, curent status and future prospects for long-term conservation. Selbyana, 14: 89-104

Rasmussen H.N. 1995. Terrestrial orchids from seed to mycotrophic plant. Cambridge, Cambridge University press: 444 p; DOI: 10.1017/CBO9780511525452

Snow R. 1985. Improvements in methods for the germination of orchid seed. American Orchid Society Bulletin, 54: 178-181




DOI: http://dx.doi.org/10.14720/aas.2015.105.1.10

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