Determination of an optimal priming duration and concentration protocol for pepper seeds (Capsicum annuum L.)

Hassen ALOUI, Maher SOUGUIR, Chérif HANNACHI

Abstract


Seed priming is a simple pre-germination method to improve seed performance and to attenuate the effects of stress exposure. The objective of this study was to determinate an optimal priming protocol for three pepper cultivars (Capsicum annuum L.): ‘Beldi’, ‘Baklouti’ and ‘Anaheim Chili’. Seeds were primed with three solutions of NaCl, KCl and CaCl2 (0, 10, 20 and 50 mM) for three different durations (12, 24 and 36h). Control seeds were soaked in distilled water for the same durations. After that, all seeds were kept to germinate in laboratory under normal light and controlled temperature. Results indicated that priming depends on concentration, duration and cultivar. The best combinations that we obtained were: KCl priming (10 mM, 36h) for ‘Beldi’ cultivar, CaCl2 priming (10 mM, 36h) for ‘Baklouti’ cultivar and finally NaCl priming (50 mM, 24h) for ‘Anaheim Chili’ cultivar. Generally, priming had an effect on total germination percentage, mean germination time, germination index and the coefficient of velocity compared to control seeds. The beneficial effect of seed priming could be used for improving salt tolerance on germination and early seedling growth for pepper cultivar.

Keywords


capsicum annuum; seed production; seed collection; germination; seed treatment; stress; pregermination

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References


Afzal, I., Basra, S. M. A., Hameed, A., & Farooq, M. (2006). Physiological enhancements for alleviation of salt stress in wheat. Pak. J. Bot , 38 (5), 1649- 1659.

Almansouri, M., J.M. Kinet and S. Lutts (2001). Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.) . Plant Soil. 231: 243–254. DOI: 10.1023/A:1010378409663

Association of Official Seed Analysis (AOSA), “Seed Vigor Testing Handbook. Contribution,” No.32 to the handbook on Seed Testing, published by AOSA and SCST, USA, 1983.

Bradford, K.J. 1986. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience 21:1105-1112.

Bray, C.M., P.A. Davison, M. Ashraf and R.M. Taylor, 1989. Biochemical changes during osmopriming of leek seeds. Ann. Bot., 63: 185 ‒ 193.

Cano, E.A., M.C. Bolarin, F. Perez-Alfocea and M. Caro. 1991. Effect of NaCl priming on increased salt tolerance in tomato. J. Hort. Sci., 66: 621-628.

Cayuela E., Perez-Alfocea F., Caro M. and Bolarin M.C.1996. Priming of seeds with NaCl induces physiological changes in tomato plants grown under salt stress. Physiology Plant., 96:231–236. DOI: 10.1111/j.1399-3054.1996.tb00207.x

Coolbear P, Grierson D (1979). Studies on the change in the major nucleic acid components of tomato seed (Lycopersicon esculentum) resulting from osmotic presowing treatment.Journal of Exp. Bot., 30:1153- 1162. DOI: 10.1093/jxb/30.6.1153

Elouaer MA, Hannachi C (2012). Seed priming to improve germination and seedling growth of safflower (Carthamus tinctorius ) under salt stress. Eurasian J. Biosci. 6:76-84. DOI: 10.5053/ejobios.2012.6.0.9

Farhoudi, R. and F. Sharifzadeh. 2006. The effect of NaCl priming on salt tolerance in canola (Brassica napus L.) seedlings grown under saline conditions. Indian J. Crop Science, 1(1-2):74-78.

Fu JR, Lu SH, Chen RZ, Zhang BZ, Liu ZS, Cai DY (1988). Osmoconditioning of peanut (Arachis hypogaea L) Seed with PEG to improve vigor and some biochemical activities. Seed Sci and Tech 16: 197-212.

Hopper NW, Overholt JR, Martin JR (1979). Effect of cultivar, temperature and seed size on the germination and emergence of soy beans ( Glycine max L.). Merr. Ann. Bot. 44: 301-308.

Iqbal, M., M. Ashraf, A. Jamil and S. Ur-Rehman. 2006. Does seed priming induce changes in the levels of some endogenous plant hormones in hexaploid wheat plants under salt stress? Journal of Integrative Plant Biology, 48(2): 181-189. DOI: 10.1111/j.1744-7909.2006.00181.x

Kaya MD, Okcu G, Atak M, Cikili Y, Kolsarici O (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.) Eur. J.Agron. 2006, 24: 291-295. DOI: 10.1016/j.eja.2005.08.001

Khan, H. A., C.M. Ayub, M.A. Pervez, R.M. Bilal, M.A. Shahid and K. Ziaf. 2009. Effect of seed priming with NaCl on salinity tolerance of hot pepper (Capsicum annuum L.) at seedling stage. Soil and Environment, 28(1): 81-87.

Levitt J. 1980. Responses of Plants to Environmental Stresses, vol. II. Academic Press, New York.

Mazor L, Perl M, Negbi M (1984). Changes in some ATP-dependent activities in seed during treatment with polyethylene glycol and during redrying process. J Exp Bot 35: 1119-1127. DOI: 10.1093/jxb/35.8.1119

Moradi Dezfuli P, Sharif-zadeh F, Janmohammadi M (2008). Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). ARPN Journal of Agricultural and Biological Science. 3(3): 22-25.

Passam, H.C. and D. Kakouriotis (1994). The effects of osmoconditioning on the germination, emergence and early plant growth of cucumber under saline conditions. Scientia Horticulturae 57: 233-240. DOI: 10.1016/0304-4238(94)90143-0

Ruan S, Xue Q, Tylkowska K (2002).Effects of seed priming on germination and health of rice (Oryza sativa L) seeds. Seed Science and Technol 30:451- 458.

Sadeghi H., Khazaei F., Yari L., Sheidaei S., 2011. Effect of seed osmopriming on seed germination behavior and vigor of soyebean (Glycine max L.). ARPN Journal of Agricultural and Biological Science, 6 (1): 39-43.

Scott SJ, Jones RA, Williams WA (1984). Review of data analysis methods for seed germination. Crop Sci., 24: 1192–1199. DOI: 10.2135/cropsci1984.0011183X002400060043x

Shaha R, Mandal AK, Basu RN (1990). Physiology of seed invigoration treatments in soybean (Glycine max L). Seed Sci and Tech 18: 269-276.

Soughir, M., Aymen, E. M., & Cherif, H. (2012). Effect of NaCl priming duration and concentration on germination behavior of fenugreek. Albanian Journal of Agricultural Sciences, 11 (4): p 193- 198. DOI: 10.2478/v10298-012-0085-7

Soughir, M., Elouaer, M. A., & Hannachi, C. (2013). The Effect of Nacl Priming on Emergence, Growth and Yield of Fenugreek Under Saline Conditions.Cercetar i agronomice in Moldova, 46 (2), 73-83.

Varier, A., Vari, A.K., Dadlani, M.2010. The subcellular basis of seed priming. Current Science, Bangalore, v.99, n.4, p450-456.

Yamauchi M, Winn T (1996). Rice seed vigor and seedling establishment in anaerobic soil. Crop Science, 36: 680–6. DOI: 10.2135/cropsci1996.0011183X003600030027x




DOI: http://dx.doi.org/10.14720/aas.2014.103.2.6

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