Vpliv UV-B sevanja na antioksidacijsko aktivnost, sestavo eteričnih olj in fiziološke parametre roženkrauta (Pelargonium graveolens L’Hér.)

Marjan AZARAFSHAN, Maryam PEYVANDI, Hossein ABBASPOUR, Zahra NOORMOHAMMADI, Ahmad MAJD

Povzetek


Roženkravt (Pelargonium graveolens L’Hér.) je med gojenimi pelargonijami pomembna aromatična in zdravilna rastlina zaradi vsebnosti eteričnih olj (EOS). Namen te raziskave je bil ovrednotiti učinke UV-B sevanja na njene rastne parametre, vsebnost eteričnih olj, antioksidacijsko aktivnost eteričnih olj in antioksidacijskih encimov, vsebnost ogljikovih hidratov, listnih pigmentov in celokupnih beljakovin. Potaknjenci iz v loncih vzgojenih rastlin so bili izpostavljeni 0; 0,12; 0,26, in 0,38 W m-2 UV-B sevanja. Aktivnost antioksidacijskih encimov, vsebnost ogljikovih hidratov, beljakovin in listnih barvil so bile izmerjene s spektrofotometričnimi metodami. Sestava eteričnih olj je bila analizirana z GC-MS metodo. Antioksidacijska aktivnost eteričnih olj je bila analizirana z aktivnostjo lovilcev prostih radikalov z uporabo 2, 2-difenil-1-pikrilhidrazila (DPPH). Rezultati so pokazali, da se je vrednost parametrov kot so sveža in suha masa listov, višina rastlin, število listov, vsebnost klorofila, beljakovin in celokupnih ogljikovih hidratov značilno (P ≤ 0,05) zmanjšala, če so bile rastline izpostavljene povečanemu UV sevanju. Nasprotno sta se vsebnost karotenoidov in aktivnost antioksidacijskih encimov povečali. Kemijska sestava eteričnih olj je pokazala, da so bile njihove glavne sestavine pri vseh obravnavanih rastlinah citronelol, geraniol in citronelil format. Antioksidacijska aktivnost eteričnih olj se je povečala z naraščanjem jakosti UV-B sevanja. Maksimalna in minimalna IC50 vrednost eteričnih olj sta bili dobljeni pri kontrolnih rastlinah, izpostavljenih UV-B sevanju 0,38 W m-2.

Ključne besede


Pelargonium graveolens; UV-B sevanje; eterična olja; antioksidacijska aktivnost; antioksidacijski encimi

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2019.113.2.2

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