Vzpostavitev primarne celične kulture konjskih keratinocitov iz biopsij

Jernej OGOREVC, Tjaša LAPANJA, Klavdija POKLUKAR, Natalija TOMINŠEK, Peter DOVČ

Povzetek


Primarna celična linija iz kožnega tkiva lahko služi za imunološke, proteomske in genomske raziskave kot in vitro model kože. Cilj naše študije je bila vzpostavitev primarne celične linije keratinocitov iz biopsije kožnega tkiva dveh konjev. Primarno celično kulturo smo pridobili z mehansko in encimsko razgradnjo tkiva in z metodo izraščanja celic. Rezultat je bila heterogena kultura keratinocitov in fibroblastov. Za imunološko karakterizacijo smo uporabili protitelesa proti citokeratinu 14 in vimentinu ter poskusili obogatiti delež keratinocitov v kulturi. Uspešno smo pridobili primarno celično linijo keratinocitov, ki je potencialno uporabna za preučevanje kožnih bolezni konjev, kot živalski model za preučevanje človeških bolezni in za testiranje kozmetičnih izdelkov ter zdravil.

Ključne besede


molekularna biologija; primarna celična kultura; koža; konji; keratinocite

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2015.106.2.3

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