Pošlji prispevek (e-pošta) Antioksidacijska aktivnost in akutna toksičnost dveh sort vodnjače (Lagenaria siceraria (Molina) Standl.) iz Sudana
Povzetek
Raziskava je bila izvedena za ovrednotenje antioksidacijske sposobnosti in akutne toksičnosti metanolnih izvlečkov suhih semen dveh sort vodnjače (Lagenaria siceraria (Molina) Standl.)), sladke ‘Water Jug’ (WJ) in grenke vodnjače ‘Basket Ball’ (BB). V izvlečkih semen so bili analizirani celokupna vsebnost fenolov (TPC), celukupna vsebnost flavonoidov (TFC), velikost redukcije železa (FRAP) in lovilna aktivnost stabilnega prostega radikala 2,2-difenil-1-pikrilhidrazila (DPPH). Oba izvlečka sta bila potem preiskušena na akutno toksičnost z odmerkoma 2 in 5 g kg-1. 60 podganjih samic in samcev je bilo razdeljeno glede na spol v pet skupin. Vsaka od štirih skupin je dobivala velike in majhne odmerke izvlečka semen, peta skupina pa je dobila 10 % Tween 20 kot kontrolo. Ledvice in jetra vseh podgan so bile ocenjene po biokemičnih in histopatoloških parametrih. Izvleček semen WJ je pokazal večjo aktivnost TPC, FRAP in DPPH v primerjavi s semeni BB, a rezultati TFC so bili obratni. Na živalih ni bilo opaziti nobenih znakov zastrupitve. Biokemični parametri so bili znotraj normalih meja z rahlim povečanjem vsebnosti bilirubina in aktivnosti alanin aminotransferaze (ALT). Tudi histološki pregled ledvic in jeter je pokazal normalno zgradbo. Zaključimo lahko, da imajo izvlečki suhih semen WJ in BB veliko antioksidacijsko aktivnost, kar obeta njihovo uporabo pri blaženju oksidacijskega stresa.
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DOI: http://dx.doi.org/10.14720/aas.2020.116.2.1031
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