Pošlji prispevek (e-pošta) Določanje odzivnih genov in analiza genov, ki jih v promotorskih območjih riža (Oryza sativa L.) inducirajo bakterijski cis-regulatorni elementi
Povzetek
Bakterijski ožig riža, ki ga povzroča vrsta Xanthomonas oryzae pv. oryzae (Xoo) je ena izmed najbolj kritičnih bolezni riža. Za preučevanje odzivnih genov pri rižu na bakterijski stres so bili pridobljeni podatkovni seti eksperimenta mikromrež iz podatkovne baze GEO. Za določanje odzivnih genov na bakterijski stres so bila uporabljena bioinformacijska orodja, katerih rezultati so bili predstavljeni v obliki barvnih vizualizacij, genske ontologije, genskega omrežja in napovedi cis-elementov. Skoraj pri večini odzivnih genov je bilo izražanje zavrto po približno 3 urah in aktivirano po približno 24 urah kot odziv na bakterijski stress pri sortah riža kot so Oryza sativa subspecies japonica ‘IR64’, ‘IRBB5’, ‘IRBB7’ in ‘Y73’. Genska ontologija je pokazala, da so ti geni vključeni v različne biološke procese, vključno s translacijo in presnovnimi procesi proteinov v celici. Analiza genskega omrežja je pokazala, da so geni, ki so se izrazili kot odziv na okužbo s patogenom (Xoo) vključevali translacijo proteinov, eukariontske iniciacijske faktorje (eIFs), ribosomalne proteine, protein ubikvitinin in MAPK gene. Geni, ki se izrazijo kot odziv na bakterijski stres omogočajo rastlini usklajevanje med sitezo in razgradnjo beljakovin, kar ji omogoča nadaljno rast in razvoj. Zaporedja nukleotidov kot so TATA in CAAT območja so imela največje število cis elementov povezanih z bakterijskim stresom. Ti geni lahko dajo nove vpoglede v regulatorne mehanizme pri odzivu riža na biotski stres. Identifikacija na bakterijski stres tolerantnih in odzivnih genov lahko pripomore pri molekularnem žlatnjenju novih sort riža, tolerantnih na bakterijski stres.
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DOI: http://dx.doi.org/10.14720/aas.2020.116.1.1035
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