Pošlji prispevek (e-pošta) Vloga dodajanja glicin betaina na nekatere antioksidacijske aktivnosti transformiranega in navadnega tobaka (Nicotiana tabacum L.) v razmerah in vitro solnega stresa
Povzetek
Glicin betain je ozmoprotektant, ki vzpodbuja toleranco rastlinskih celic na okoljski stres. V tej raziskavi so bili preučevani učinki dodajanja glicin betaina na nekatere antioksidacijske aktivnosti tobaka, ki ima prekomerno izražanje P5CS gena. Sterilne sejanke tobaka s štirimi do šestimi listi so bile premeščene v MS gojišče, ki je vsebovalo 0, 100, in 200 mM NaCl, nakar je bil dodan s pršenjem nadzemnih delov glicin betain (20 in 40 mg l-1). Po štirih tednih je obravnavanje z glicin betainom pospešilo antioksidacijsko sposobnost rastlin z aktivacijo katalaze (CAT), superoksid dizmutaze (SOD) in askorbat peroksidaze (APX). Nasprotno sta se vsebnosti H2O2 in MDA zmanjšali po obravnavanju z glicin betainom v podobnih razmerah. Dodajanje glicin betaina lahko v razmerah solnega stresa izboljša toleranco na stres transformiranih in netransformiranih rastlin. Rezultati raziskave so še pokazali, da je bil pozitivni učinek glicin betaina večji pri transformiranih rastlinah. Po drugi strani ti rezultati nakazujejo, da sinergistični učinki glicin betaina in prolina v rastlinah pospešujejo antioksidacijski obrambni sistem v transformiranih rastlinah, ki imajo prekomerno izraženo delovanje P5CS gena.
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DOI: http://dx.doi.org/10.14720/aas.2021.117.3.1056
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