Pošlji prispevek (e-pošta) Vpliv različnih postopkov fermentacije na produkcijo biomase micelija, ekstra in itracelularnih polisaharidov in na antioksidacijsko aktivnost kokonovega glavatca (Cordyceps militaris (L.) Fr., seva AG-1, PSJ-1)
Povzetek
Biomasa micelija, antioksidacijska aktivnost in produkcija zunaj- in znotraj celičnih polisaharidov [EPS, IPS] so bile ovrednotene pri kokonovem glavatcu (Cordyceps militaris (L.) Fr., seva AG-1, PSJ-1) v razmerah različnih submerznih tekočih kultur (SLC) . Pri 24 0C sta bili biomasa micelija in produkcija polisaharidov pri obeh sevih optimalni v gojišču PVC in stabilnih ramerah; (AG-1: 21,85 ± 1,00; PSJ-1: 18,20 ± 1,84 g l-1 ), po sušenju v pečici na 40 oC (AG-1: 25,95 ± 0,84, PSJ-1: 23,55 ± 0,69 mg g-1) v primerjavi z ekstrakcijo z vročo vodo (AG-1: 7,07 ± 0,15, PSJ-1: 7,39 ± 0,61 mg g-1). Največja biomasa in produkcija obeh tipov polisaharidov (EPS in IPS) sta bili doseženi pri začetnem pH 6,7: AG-1: 12,92 ± 0,33; 209,70 ± 1,56; 32,62 ± 0,87; PSJ-1: 9,03 ± 0,24 g l-1; 198,16 ± 0,85 mg g-1; 30,63 ± 1,96 mg g-1. Uporaba kokosovega olja (3,5 %) je povečala biomaso in produkcijo polisaharidov v obeh sevih in sicer: 8,27 ± 0,09; 8,01 ± 0,01 g l-1; 1208,00 ± 8,60: 1110,40 ± 7,20 mg g-1; 32,43 ± 0,49; 29,74 ± 0,44. Oba načina gojenja in metode sušenja v pečici so imeli značilen vpliv na aktivnost nevtralizacije H2O2 in prostih radikalov, aktivnost ABTS.+ radikala in peroksidacijo lipidov. Imeli so tudi vpliv na vsebnost celokupnih flavonoidov in fenolov. Uporaba surove submerzne tekoče culture in sušenje obeh sevov v pečici sta dali izvlečke s potencialno antioksidacijsko aktivnostjo, kar nakazuje terapevtsko uporabo polisaharidov iz obeh sevov kokonovega glavatca.
Ključne besede
Celotno besedilo:
PDF (English)Literatura
Arumagam, P, Ramamurthy, P, Santhiya, ST, & Ramesh, A. (2006). Antioxidant activity measured in different solvent fractions obtained from Mentha spicataLinn: An analysis by ABTS .+ decolorization assay. Asia Pacific Journal of Clinical Nutrition, 15, 119–124.
Atlas, RM. (1993). Handbook of microbiological media. CRC Press, Bota Raton.
Cazzi, R, Ricardy, R, Aglitti, T, Gat ta, V, Petricone, P, & De, Salvia, R. (1997). Ascorbic acid and β-carotene as modulators of oxidative damage. Carcinogenesis, 18, 223-228. https://doi.org/10.1093/carcin/18.1.223
Chan, EWC, Lim, YY, Wong, SK, Lim, KK, Tan, SP, Lianto, FS, & Yong, MY. (2009). Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chemistry, 113, 166–172. https://doi.org/10.1016/j.foodchem.2008.07.090
Chun-Lun Wang, Chung-Jen Chiang, Yun-Peng Chao, Bi Yu, & Tzu-Tai Lee. (2015). Effect of Cordyceps militaris water medium on production performance, egg traits and egg yolk cholesterol of laying hens. Poultry Science Journal, 52, 188-196,. https://doi.org/10.2141/jpsa.0140191
Chunyan, XIE, Gaixia, LIU, Zhenxin, GU, Gongjian, FAN, Lei, ZHANG, & Yingjuan, GU. (2009). Effects of culture conditions on mycelium biomass and intracellular cordycepin production of Cordyceps militaris in natural medium. Annals of Microbiology, 59 (2), 293-299. https://doi.org/10.1007/BF03178331
Cuzzocrea, S, Riley, DP, Caputi, AP, & Salvemini, D. (2001). The American Society of Pharmacology and Experimental Therapeutics. Pharmacological Reviews, 53, 135-159.
Dang, HN, Wang, CL, & Lay, HL. (2018). Effect of nutrition, vitamin, grains, and temperature on the mycelium growth and antioxidant capacity of Cordyceps militaris (strains AG-1 and PSJ-1). Journal of Radiation Research and Applied Sciences, 11(2), 130-138. https://doi.org/10.1016/j.jrras.2017.11.003
Dong, CH, & Yaoa, YJ. (2007). In vitro evaluation of antioxidant activities of aqueous extracts from natural and cultured mycelia of Cordyceps sinensis. LWT-Food Science and Technology, 41 (2008), 669-677. https://doi.org/10.1016/j.lwt.2007.05.002
Dong, J, Zhang, M, Lu, L, Sun, L, & Xu, M. (2012). Nitric oxide fumigation stimulates flavonoid and phenolic accumulation and enhances antioxidant activity of mushroom. Food Chemistry, 135(3), 1220-1225. https://doi.org/10.1016/j.foodchem.2012.05.055
Elmastas, M, Isildak, O, Turkekul, I, & Temur, N. (2007). Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. Journal of Food Composition Analysis, 20, 33-45. https://doi.org/10.1016/j.jfca.2006.07.003
Fang, QH, & Zhong, JJ. (2002). Submerged fermentation of higher fungus Ganoderma lucidum for production of valuable bioactive metabolites-ganoderic acid and polysaccharide. Biochemical Engineering Journal, 10(1), 61-65. https://doi.org/10.1016/S1369-703X(01)00158-9
Furuta, S, Nishiba, Y, & Suda, I. (1997). Fluorometric assay for screening antioxidative activity of vegetables. Journal of Food Science, 62(3), 526-528. https://doi.org/10.1111/j.1365-2621.1997.tb04422.x
Girotti, AW. (1990). Photodynamic lipid peroxidation in biological systems. Photochemistry and Photobiology, 51(4), 497-509. https://doi.org/10.1111/j.1751-1097.1990.tb01744.x
Halliwell, B, Gutteridge, JMC, & Cross, CE. (1992). Free-Radicals, Antioxidants, and Human-Disease - Where Are We Now. Journal of Laboratory and Clinical Medicine, 119(6), 598-620.
Hsieh, C, Liu, CJ, Tseng, MH, Lo, CT, & Yang, YC. (2006). Effect of olive oil on the production of mycelial biomass and polysaccharides of Grifola frondosa under high oxygen concentration aeration. Enzyme and Microbial Technology, 39(3), 434-439. https://doi.org/10.1016/j.enzmictec.2005.11.033.
Hsieh, C, Tsai, MJ, Hsu, TH, Chang, DM, & Lo, CT. (2005). Medium optimization for polysaccharide production of Cordyceps sinensis. Biotechnology and Applied Biochemistry, 120(2), 145-157. https://doi.org/10.1385/ABAB:120:2:145
Ing-Lung Shih, Kun-Lin Tsai, & Chienyan Hsieh. (2007). Effects of culture conditions on the mycelial growth and bioactive metabolite production in submerged culture of Cordyceps militaris. Biochemical Engineering Journal, 33, 193-201. https://doi.org/10.1016/j.bej.2006.10.019
Isildak Ö, Turkekul I, Elmastas M, & Tuzen M. (2004). Analysis of heavy metals in some wild -grown edible mushrooms from the middle Black Sea region, Turkey. Food Chemistry, 86, 547-552. https://doi.org/10.1016/j.foodchem.2003.09.007
Jia, Z, Tang, M, & Wu, J. (1999). The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
Jo, WS, Choi, YJ, Kim, HJ, Lee, JY, Nam, BH, Lee, JD, Jeong, MH. (2010). The Anti-inflammatory Effects of Water Extract from Cordyceps militaris in Murine Macrophage. Mycobiology, 38(1), 46-51. https://doi.org/10.4489/MYCO.2010.38.1.046
Junqiao Wang, Lijiao Kan, Shaoping Nie, Hai hong Chen, Steve, WC, Aled, O, Xie, My. (2015). A comparison of chemical composition, bioactive components and antioxidant activity of natural and cultured Cordyceps sinensis. LWT - Food Science and Technology, 63, 2-7. https://doi.org/10.1016/j.lwt.2015.03.109
Kang, C, Wen, TC, Kang, JC, Meng, ZB, Li, GR, & Hyde, KD. (2014). Optimization of Large-scale culture conditions for the production of cordycepin with Cordyceps militaris by liquid static culture. Scientific World Journal, 4, 1-15. https://doi.org/10.1155/2014/510627
Kaur, C, & Kapoor, HC. (2002 ). Anti-oxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science&Technology, 37(2), 153-161. https://doi.org/10.1046/j.1365-2621.2002.00552.x
Kiho T, Itahashi S, Sakushima M, Matsunaga T, Usui S, Ukai S, Ishiguro Y. (1997). Polysaccharide in fungi, XXXVIII. Anti-diabetic activity and structural feature of galactomannan elaborated by Pestalotiopsis species. Biological and Pharmaceutical Bulletin, 20, 118–121. https://doi.org/10.1248/bpb.20.118
Kojima I, Yoshikawa H, Okazaki M, & Terui Z. (1972). Studies on riboflavin production by Eremothecium ashbyii. Journal of Fermentation Technology, 50, 716–723.
Lee, TT, Wang, CL, & Yu, B. (2013). Functional components of spent Cordyceps militaris composts and its effects in egg quality and egg cholesterol. World’s Poultry Science Journal, 69, 15-19.
Leung, PH, & Wu, JY. (2007). Effects of ammonium feeding on the production of bioactive metabolites (cordycepin and exopolysaccharides) in mycelial culture of a Cordyceps sinensis fungus. Journal of Applied Microbiology, 103, 1942–1949. https://doi.org/10.1111/j.1365-2672.2007.03451.x
Mao, XB, Eksriwong, T, Chauvatcharin, S, & Zhong, JJ. (2005). Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris. Process Biochemistry, 40, 1667-1672. https://doi.org/10.1016/j.procbio.2004.06.046
Mediani, A, Abas, F, Khatib, A, & Tan, C. (2013). Cosmos caudatus as a potential source of polyphenolic compounds: Optimisation of oven drying conditions and characterisation of its functional properties. Molecules, 18, 10452–10464. https://doi.org/10.3390/molecules180910452
Nabavi, SM, Ebrahimzadeh, MA, Nabavi, SF, Hamidinia, A, & Bekhradnia, AR. (2008). Pharmacology, 2, 560-567.
Nielsen, J, Johansen, CL, Jacobsen, M, Krabben, P, & Viladsen, J. (1995). Pellet formation and fragmentation in submerged cultures of Penicillium chrysogenum and its relation to penicillin production. Biotechnology Progress, 11, 93–98. https://doi.org/10.1021/bp00031a013
Park J.P, Kim SW, Hwang HJ, Choi YJ, & Yun JW. (2002). Stimulatory effect ofplant oils and fatty acids on the exo-biopolymer production in Cordyceps militaris. Enzyme and Microbial Technology, 31 250–255. https://doi.org/10.1016/S0141-0229(02)00099-6
Park, JP, Kim, SW, Hwang, HJ, & Yun, JW. (2001). Optimization of submerged cultureconditions for the mycelia growth and exo-biopolymer production by Cordyceps militaris. Letters in Applied Microbiology, 33, 76–81. https://doi.org/10.1046/j.1472-765X.2001.00950.x
Park, JP, Kim, YM, Kim, SW, Hwang, HJ, Choi, YJ, Lee, YS, Yun, JW. (2002). Effect of aeration rate on the mycelial morphology and exo-biopolymer production in Cordyceps militaris. Process Biochemistry, 37,1257-1262. https://doi.org/10.1016/S0032-9592(02)00005-5
Re, R, Pellegrini, N, Proteggente, A, Pannala, A, Yang, M, & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Rice Evans, C, & Miller, NJ. (1997). Factors influencing the antioxidant activity determined by the ABTS radical cation. Free Radical Research, 26, 195-199. https://doi.org/10.3109/10715769709097799
Rukachaisirikul, V., Pramjit, S., Pakawatchai, C, Isaka, M., & Supothina, S. (2004). 10-membered macrolides from the insect pathogenic fungus Cordyceps militaris BCC 2816. Journal of Natural Products, 67, 1953-1955. https://doi.org/10.1021/np0401415
Sapan Kumar Sharma, Nandini Gautam & Narender Singh Atri. (2015). Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae. Bmc Complementary and Alternative Medicine, 15, 446. https://doi.org/10.1186/s12906-015-0967-y
Schisler, LC, & Volkoff, O. (1977). The effect of safflower oil on mycelial growth of Boletaceae in submerged liquid cultures. Mycologia, 69, 118–125. https://doi.org/10.1080/00275514.1977.12020038
Sharma, SK, Gautam, N, & Atri, NS. (2015). Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.) Durieu & Mont.”, Evidence-Based Complementary and Alternative Medicine, 2015, 462-864. https://doi.org/10.1155/2015/462864
Shen, QY, & Shen, QY. (2001). Study on the resisting oxygen free radical and hydroxyl free radical effect of Cordyceps militaris. Guihaia, 21, 252−254
Shih, IL, Tsai, KL, & Hsieh, C. (2007). Effects of culture conditions on the mycelial growth and bioactive metabolite production in submerged culture of Cordyceps militaris. Biochemical Engineering Journal, 33, 193–201. https://doi.org/10.1016/j.bej.2006.10.019
Singleton, VL, & Rossi, JAJr. (1965). Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
Stevens, RB. (1981). Mycology guidebook. University of Washington Press, Seattle.
Yu Xiao, Guang liang Xing, Xin Rui, Wei Li, Xiao hong Chen, Mei Jiang, & Ming sheng Dong. (2014). Enhancement of the antioxidant capacity of chickpeas by solid state fermentation with Cordyceps militaris SN-18. Journal of functional foods, 10, 210-222. https://doi.org/10.1016/j.jff.2014.06.008
Yuxiang Gu, Zunsheng Wang, & Qinsheng Yuan. (2006). The Varieties of Antioxidant Activity of Cordyceps militaris During the Submerged Fermentation. Electronic Journal of Biology, 2(2), 30-33.
Zhong, SM, Du, M, Chen, WB, & Zhang, S. (2011). Liquid culture conditions for promoting cordycepin secreted from Cordyceps militaris mycelia. Mycosystema, 30(2), 229-234.
DOI: http://dx.doi.org/10.14720/aas.2020.116.2.1104
Povratne povezave
- Trenutno ni nobenih povratnih povezav.
Avtorske pravice (c) 2020
##submission.license.cc.by-nc-nd4.footer##
Acta agriculturae Slovenica je odprtodostopna revija, ki objavlja pod pogoji licence Creative Commons Priznanje avtorstva (CC BY).
eISSN 1854-1941