Pošlji prispevek (e-pošta) Klonsko razmnoževanje vrste Tetragonolobus palaestinus Bioss: jordanske zdravilne rastline
Povzetek
Vrsta Tetragonolobus palaestinus Bioss (Aljalaton) je jordanska zdravilna rastlina, ki se lahko uporablja za blaženje bolečin v želodcu in zdravljenje nekaterih okužb. Namen raziskave je bil vzpostaviti optimalen način in vitro razmnoževanja te rastline. Preučevani so bili dejavniki in vitro gojenja (vkoreninjenja, tvorbe poganjkov) in aklimatizacije sadik te rastline z uporabo različni rastnih regulatorjev. Za in vitro tvorbo poganjkov so bili uporabljeni različni citokinini in sicer benzilamino purin (BAP), kinetin (TDZ) in zeatin v naraščajoči koncentraciji (0,0; 0,3; 0,6; 0,9; 1,2; 1,5 in 2,0 mg.l-1). Uporaba benzilamino purina (0,3 mg l-1) je dala pri gojenju na Murashige in Skoog (MS) gojišču največ poganjkov, dva na izseček. Učinek različnih koncentracij rastnih regulatorjev (IBA-indol-3-maslene kisline, IAA -indol-3-ocetne kisline in naftalen ocetne kisline NAA) je bil ovrednoten pri in vitro vkoreninjenju. Največje število korenin (4,06 korenin/izseček) je bilo dobljeno na MS gojišču, z dodatkom 0,3 mg l-1 IBA. Vse rastline (100 %) so po obdobju aklimatizacije rastle normalno. Na osnovi teh rezultatov je bil prvič optimiziran enostaven protokol za in vitro gojenje te vrste, ki bi se lahko uporabil v nadaljnih raziskavah na celičnih kulturah in produkciji aktivnih sekundarnih metabolitov.
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DOI: http://dx.doi.org/10.14720/aas.2022.118.3.1208
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