UČINKOVITA INDUKCIJA TRANSGENIH LASASTIH KORENIN PRI NAVADNEM POTROŠNIKU: NOVA UPORABA TRADICIONALNEGA ZELIŠČA

Sara Kabirnataj, Ghorbanali Nematzadeh, Jafar Zolala, Ahmad Farhad Talebi

Povzetek


Korenine lahko z bakterijo Agrobacterium rhizogenes spremenimo v “zelene celične tovarne”, ki proizvajajo heterologne proteine, uporabne v farmaciji. V tej raziskavi je bila preučevana uporaba štirih sevov bakterije A. rhizogenes (A4, ATCC15834, ATCC11325 in A13) v kombinaciji s tremi ko-kultivacijskimi gojišči (MS, B5, LS) za vzpostavitev učunkovitega transformacijskega sistema za navadni potrošnik (Cichorium intybus L.). Največja indukcija lasastih korenin je bila dosežena z uporabo seva A13. Opazovanja so potrdila, da je bilo za rast lasastih korenin učinkovitejše MS gojišče. Biomasa lasastih korenin, okuženih s sevom A13 je bila 1.10 g l-1 na MS gojišču, kar je bilo značilno več kot pri rasti korenin na gojiščih LS in B5 (0,88 in 0,72 g l-1). Gen za beta-glukuronidazo (GUS) je bil vnešen z A13 sevom, ki je vseboval pCAMBIA1304 binarni vektor. Izsledki so pokazali, da je bila največja frekvenca transformacije (63,15 %) dosežena z uporabo A13 seva in MS gojišča. Detekcija GUS in hptII genov s PCR in GUS histokemično lokalizacijo je potrdila njuno vključitev v lasaste korenine. Celoten proces je bil uspešno optimiziran kot predstopnja v obdelavi celic lasastih korenin navadnega potrošnika za izboljšanje sposobnosti tvorbe sekundarnih metabolitov.

Ključne besede


navadni potrošnik, A. rhizogenes, lasaste korenine, GUS, A13

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2016.107.2.06

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