Pošlji prispevek (e-pošta) Primerjalni učinki kantaridina in endotala na izražanje genov in aktivnost antioksidacijskih encimov pri navadnem potrošniku (Cichorium intybus L.)
Povzetek
Kantaridin in njegov analog endotal sta poznana po fitotoksičnih učinkih, pri katerih njun mehanizem delovanja vsebuje inhibicijo fosfataz. Encimi in antioksidacijske spojine tvorijo bariero proti fitotoksičnim spojinam. Katalaza in peroksidaza sta med najpomembnejšimi antioksidacijskimi encimi. Navadni potrošnik (Cichorium intybus L.) se tradicionalno uporablja kot zdravilna rastlina zaradi vsebnosti različnih fitokemikalij in encimov. V raziskavi so bili preučevani primerjalni učinki kantaridina in endotala v koncentracijah 2,5, 5,5 in 10 µg ml-1 na spremembe v izražanju genov za katalazo in glutation peroksidazo. Aktivnosti teh dveh encimov so bile ocenjene v poganjkih in koreninah navadnega potrošnika. Izsledki so pokazali, da se je izražanje genov za katalazo in glutation peroksidazo bolj povečalo v vzorcih, ki so bili tretirani s kantaridinom kot v tistih tretiranih z endotalom v primerjavi s kontrolo, tako v poganjkih (najznačilnejši učinek je imela koncentracija kantaridina 2,5 µg ml-1) kot v koreninah (najznačilnejši učinek je imela koncentracija kantaridina 5,5 µg ml-1). Dodatno sta aktivnost katalaze in koncentracija kantaridina (2,5 µg ml-1) v vzorcih poganjkov vodili k intenzivnejši stimulaciji katalaze in glutation peroksidaze v primerjavi z vzorci korenin. Predpostavljamo, da imata kantaridin in endotal negativni učinek na izražanje genov antioksidacijskih encimov in na njihovo absoprcijo.
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DOI: http://dx.doi.org/10.14720/aas.2020.116.1.1232
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