IN VITRO UPORABA INTEGRALNEGA SELEKCIJSKEGA INDEKSA ZA IZBOR NA SUŠO ODPORNIH GENOTIPOV NAVADNE PŠENICE

Ezatollah FARSHADFAR, Reza Amiri

Povzetek


Poskus je bil izveden na 20 genotipih navadne pšenice v rastni sezoni 2010-2011 na Razi University, Kermanshah, Iran. Popolno naključni načrt poskusa s šestimi ponovitvami je bil uporabljen za indukcijo kalusa, z 20 × 2 faktorskim poskusom s tremi ponovitvami pa se je ugotavljal odziv genotipov pšenice na sušni stres v razmerah in vitro. ANOVA je pokazala visoko značilne razlike med genotipi v rasti kalusa, prirastku sveže mase, hitrosti njene prirasti, vsebnosti vode v kalusu, odstotku kloroze kalusa in v vsebnosti prolina v stresnih razmerah (15 % PEG). PCA analiza je pokazala, da je integralni selekcijski indeks koreliral z indeksom rasti kalusa, s prirastkom sveže mase, hitrostjo njene prirasti in vsebnostjo prolina, kar kaže, da so te presevne metode uporabne za izbor na sušo odpornih genotipov. Pri izboru na sušo odpornih genotipov s kazalniki ospornosti na sušo v in vitro poskusu kot osnovnim merilom, sta standardna deviacija vrednosti analiziranih znakov in “biplot” analiza izločili genotipe 2, 18 in 10 kot najbolj odporne na sušo. Zaradi tega so bili ti priporočeni za uporabo kot starševske rastline za genske analize, gensko mapiranje in izboljševanje odpornosti na sušo.

Ključne besede


biplot analiza, kultura zrelega embrija, sušni stres, fiziološki indikatorji, analiza glavnih komponent, Triticum aestivum

Celotno besedilo:

PDF (English)

Literatura


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DOI: http://dx.doi.org/10.14720/aas.2016.107.2.07

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