Študij polarnosti verig različnih viroidov in njihovih kombinacij pri okuženih rastlinah hmelja

Andrej SEČNIK, Sebastjan RADIŠEK, Nataša ŠTAJNER, Jernej JAKŠE

Povzetek


Hmelj (Humulus lupulus L.) je pomembna kmetijska rastlina, ki jo pridelujemo zaradi hmeljnih storžkov, ki so osnovna sestavina pri proizvodnji piva. Hmelj pa je tudi gostiteljska rastlina štirim različnim viroidom. V naši raziskavi, kjer smo želeli proučiti naravo okuževanja s strani viroidov, smo analizirali nivo akumulacije in polarnost verig molekul viroidov. S podatki RNK sekvenciranja smo pokazali, da se viroidi v rastlini razmnožijo do ravni zasičenja, kar kaže na biološko kapaciteto rastline. Negativne spremembe na ravni akumulacije posameznih viroidov med vzorci hmelja z enojno, dvojno ali trojno okužbo kažejo na to, da je med viroidi prisoten antagonizem, pri čemer je viroid razpokanosti skorje agrumov najmanj, viroid zakrnelosti hmelja pa najbolj občutljiv na ostala dva. Kar se tiče polarnosti viroidne RNK molekule pa podatki RNK sekvenciranja kažejo, da je v povprečju več (−) oblik kot (+) oblik viroida. Pri tem najbolj izstopa viroid razpokanosti skorje agrumov. Pri rezultatih obratne transkripcije in verižne reakcije s polimerazo v realnem času pa glede polarnosti zaznamo variabilnost med različnimi kombinacijami okužb, razen v primeru viroida razpokanosti skorje agrumov, kjer se podatki o polarnosti skladajo s podatki RNK sekvenciranja.


Ključne besede


hmelj; viroidi; RT-qPCR; NGS; RNK sekvenciranje

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2020.115.1.1319

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