Pošlji prispevek (e-pošta) Določanje beljakovin v občutljivih in toleratnih linijah sončnic (Helianthus annus L.) v razmerah pomanjkanja vode
Povzetek
Pomen preučevanja okoljskih stresov in njihove vloge v napovedovanju in vrednotenju rasti in pridelka gojenih rastlin je samoumevno. Okoljski stresi so v svetovnem merilu najpomembnejši dejavniki, ki zmanjšujejo pridelke kmetijskih rastlin. Z namenom ovrednotenja vpliva pomanjkanja vode na količino beljakovin v sončnicah je bil izveden popolni naključni bločni poskus s tremi ponovitvami na Inštitutu za preučevanje oljnih semen, Karaj, Iran. Za preučevanje odziva na sušni stres občutljive (BGK221) in tolerantne linije (RGK46) sončnic so bili narejeni izvlečki beljakovin iz osmih listov treh sejank v vsaki ponovitvi z acetonsko depozicijsko metodo. Količina beljakovin v vsakem izvlečku je bila določena z bradfordovo metodo z dvo dimenzionalno elektroforezo, kjer so bile preučevane značilne razlike med progami občutljivih in tolerantnih linij. Celokupno je bilo v tolerantnih linijah ugotovljenih 467 ponovljivih prog in 417 ponovljivih prog v občutljivih linijah. Med temi beljakovinami je bilo v tolerantni liniji 6 prog (No 503, 1901, 904, 3301, 7011, 9005) in v občutljivi liniji 6 prog (No 704, 811, 3205, 4108, 7307, 9207), na katere je značilno vplival sušni stres. V obeh linijah je bila značilna posledica sušnega stresa povečanje količine beljakovin. Rezultati so pokazali, da so pri tolerantni liniji najpomembnejši mehanizmi prilagoditve na okoljski stres vzdrževanje normalne celične presnove, ohranjanje vsebnosti vode v celicah, ojačevanje celičnih struktur in antioksidacijska obramba. Raziskava je pokazala, da ima sušni stres največji učinek na beljakovine citoplazme in jedra in na presnovo beljakovin.
Ključne besede
Celotno besedilo:
PDF (English)Literatura
Adebayo, A.G., Akintoye, H.A., Aina, O.O., Olatunji, M.T., Shokalu, A.O. (2012). Assessment of organic amendments on growth and flower yield of sunflower (Helianthus annuus L.). Libyan Agricultural Research Centre Journal International, 3(1), 24-29.
Araus, J. L., Slafer, G. A., Reynolds, M. P., Royo, C. (2002). Plant breeding and drought in C3 cereals: What should we breed for? Annals of Botany, 89, 925-940. https://doi.org/10.1093/aob/mcf049
Asl, K.K., Mazaheri, D., Peighambari, S. A. (2003). Effect of four irrigation intervals on the seed yield and quantitive characteristics of sunflower cultivars. Iranian Journal Agricultural Science, 34, 293-301.
Bakalova, S., Nedeva, D., Mckee, J. (2008). Protein profiles in wheat seedlings subjected to dehydration stress. Applied Ecology and Environmental Research, 6, 37-48. https://doi.org/10.15666/aeer/0602_037048
Balbuena, T. S., Salas, J J., Martínez-Force, E., Garcees, R., Thelen, J.J. (2011). Proteome analysis of cold acclimation in sunflower. Journal of Proteome Research, 10, 2330-2346. https://doi.org/10.1021/pr101137q
Bradford, M, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analitical Biochemistry, 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
Caruso, G., Cavaliere, C., Guarino, C., Gubbiotti, R., Foglia, P., Laganà, A. (2008). Identification of changesin Triticum durum L. leaf proteome in response to salt stress by two-dimensional electrophoresis andMALDI-TOF mass spectrometry. Analitical and Bioanalitical Chemistry, 391, 381–390. https://doi.org/10.1007/s00216-008-2008-x
Dao, T. T. H., Linthorst, H. J. M., Verpoorte, R. (2011). Chalcone synthase and its functions in plant resistance. Phytochemistry Reviews, 10, 397–412. https://doi.org/10.1007/s11101-011-9211-7
Dinakar, C., Djilianov, D., Bartels, D. (2012). Photosynthesis in desiccation tolerant plants: energy metabolism and antioxidative stress defense. Plant Science, 182, 29-41. https://doi.org/10.1016/j.plantsci.2011.01.018
Dreher, K. & CALLIS, J. (2006). Ubiquitin, hormones and biotic stress in plants. Section of Molecular and Cellular Biology, Plant Biology Graduate Group Program, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. Annals of Botany, 99, 787–822. https://doi.org/10.1093/aob/mcl255
Ferro, M., Salvi, D., Brugiers, S., Miras, S., Kowalski, S., Louwagie, M. (2003). Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana. Molecular and Cellular Proteomics, 2, 325-45. https://doi.org/10.1074/mcp.M300030-MCP200
Fulda, S., Mikkat, S., Stegmann, H., Horn, R. (2011). Physiology and proteomics of drought stress acclimation in sunflower (Helianthus annuus L.). Plant Biology, 13, 632-642. https://doi.org/10.1111/j.1438-8677.2010.00426.x
Ghaffari, M., Toorchi, M., Valizadeh, M., Komatsu, S. (2013). Differential response of root proteome to drought stress in drought sensitive and tolerant sunflower inbred lines. Functional Plant Biology, 40(6), 609-617. https://doi.org/10.1071/FP12251
Goksoy, A. T., Demir, A. O., Turan Z. M., Dagustu N. (2004). Responses of sunflower (Helianthus annuus L.) to full and limited irrigation at different growth stages. Field Crop Research, 87, 167-178. https://doi.org/10.1016/j.fcr.2003.11.004
Guicherd, P., Peltier, JP., Gout, E., Bligny, R., Marigo, G. (1997). Osmoticadjustment in Fraxinus excelsior L. malate and mannitol accumulation in leaves under drought conditions. Trees, 11, 155–161. https://doi.org/10.1007/PL00009664
Gulcin, I. (2012). Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86(3), 345-391. https://doi.org/10.1007/s00204-011-0774-2
Hajduch, M., Casteel, J. E., Tang, S., Hearne, L. B., Knapp, S., Thelen, J. J. (2007). Proteomic analysis of near-isogenic sunflower varieties differing in seed oil traits. Journal of Proteome Research, 6, 3232-3241. https://doi.org/10.1021/pr070149a
Hajheidari, M., Abdollahian-Noghabi, M., Askari, H., Heidari, M., Sadeghian, S. Y., Ober, E. S., Hosseini Salekdeh, Gh. (2005). Proteome analysis of sugar beet leaves under drought stress. Proteomics, 5, 950-960. https://doi.org/10.1002/pmic.200401101
Ishfaq, M., Ali, A., Khaliq, A., Yaseen, M. (2009). Allometry Agronomic traits and yield of autumn planted sunflower hybrids under varing row spacing. Pakistan Journal of Biological Sciences, 46, 257-248
Kanlaya, K. N., Sakda, D., Chaisiri, W., Sumontip, B., Manit, K., Piyada, T. (2005). Protein profiles in response to saltstress in leaf sheaths of rice seedlings. Science Asia, 31, 403-408. https://doi.org/10.2306/scienceasia1513-1874.2005.31.403
Karam, F., Lahoud, R., Masaad, R., Kabalan, R., Breidi, J., Chalita, C., Rouphael, Y. (2007). Evapotranspiration, seed yield and water use efficiency of drip irrigated sunflower under full and deficit irrigation conditions. Agricaltural Water management, 90, 213-223. https://doi.org/10.1016/j.agwat.2007.03.009
Lawlor, D. W. & Cornic, G. (2002). Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell and Environment, 25, 275-294. https://doi.org/10.1046/j.0016-8025.2001.00814.x
Maghsoudi, B. (2006). MSc. thesis. Islamic Azad University, Tehran Science and Research.
Maghsoudi, B., Lak, Sh., Ghaffari, M., Alavifazel, M., Sakinezhad, T. (2020). Effect of agronomic traits and drought resistance indices on determination of susceptible and tolerant sunflower lines. Journal of Crop Production Research, 11(4), 2020.
Musrati, R. A., Kollarova, M., Mernik, N., Mikulasova, D. (1998). Malatedehydrogenase: distribution, function and properties. General Physiology and Biophysics, 17, 193–210.
Ndimba, B. K., Chivasa, S., Simon, W. J., Slabas, A. R. (2005). Identification of Arabidopsis salt and osmotic stress responsive proteins using two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics, 5, 4185–4196. https://doi.org/10.1002/pmic.200401282
Nezami, A., Khazaei, H. R., Boroumand, R. Z., Null, N. (2008). Effects of drought stress and defoliation on sunflower. Desert, 12, 99-104.
Passioura, J.B. (2007). The drought environment: physical, biological and agricultural perspectives. Journal of Experimental Botany, 58(2), 113-117. https://doi.org/10.1093/jxb/erl212
Pereira, S.I.A., Moreira, H., Argyars, K., Castro, P.M.L., Marques, A.P.G.C. (2016). Promotion of sunflower growth under saline water irrigation by the inoculation of beneficial microorganisms. Applied Soil Ecology, 105, 36-47. https://doi.org/10.1016/j.apsoil.2016.03.015
Rauf, S., Sadaqat, H. A., Khan, I. A., Ahmed, R. (2009). Genetic analysis of leaf hydraulics in sunflower (Helianthus annuus L.) under drought stress. Plant Soil and Environment, 55, 62–69. https://doi.org/10.17221/260-PSE
Riccardi, F., Gazeau, P., de Vienne, D., Zivy, M. (1998). Protein changes in response to progressive water deficit in maize. Plant Physiology, 117, 1253-1263. https://doi.org/10.1104/pp.117.4.1253
Wei , Y. Z., Hu, F.C., Hu, G. B., Li, X. J., Huang, X. M., Wang, H. C. (2011). Differential expression of anthocyanin biosynthetic genes inrelation toanthocyanin accumulation in the pericarp of Litchi chinensis Sonn. PLoS ONE 6, e19455. https://doi.org/10.1371/journal.pone.0019455
DOI: http://dx.doi.org/10.14720/aas.2021.117.2.1498
Povratne povezave
- Trenutno ni nobenih povratnih povezav.
Avtorske pravice (c) 2021
##submission.license.cc.by-nc-nd4.footer##
Acta agriculturae Slovenica je odprtodostopna revija, ki objavlja pod pogoji licence Creative Commons Priznanje avtorstva (CC BY).
eISSN 1854-1941