MOLEKULARNA GENETSKA ANALIZA NEKATERIH SEVERNOAFRIŠKIH GENSKIH VIROV JEČMENA

Reda Gaafar, Mai Allam, Rasha Sabry, Mahmoud Saker

Povzetek


Z izoencimskimi in RAPD markerji je bilo ovrednoteno 29 akcesij ječmena, nabranih v Severni Afriki. Za razvoj molekularnih markerjev na osnovi RT-PCR so bila uporabljena nukleotidna zaporedja genov za odpornost. Ugotovljen je bil velik polimorfizem RAPD in izoencimskih markerjev, kjer je bilo 60 % namnoženih RAPD markerjev polimorfnih. Peroksidaza je pokazala tri polimorfne lokuse (7 alelelov). Z analizo izoencimskih skupin so bile akcesije ječmena uspešno razdeljene v tri razločne geografske skupine. Raziskava RAPD je pokazala, da se egiptovske akcesije razločno povežejo v dve skupini. Tunizijske akcesije niso pokazale razločnega povezovanja, pri alžirskih pa so bile ugotovljene velike razlike. V RT-PCR analizi, je od šestih izbranih začetnih oligonukeltodiov par AF092524P1P2 uspešno namnožil dva specifična amplikona s približno 340 in 220 in 360 in 270 baznih parov pri dveh egiptovskih genotipih ječmena (El-Awamah in Awlad-Ali). Par začetnih oligonukleotidov DN988165P1P2 je pomnožil le en specifični fragment pri obeh genotipih ječmena z 250 in 270 baznimi pari. Razviti markerji se bodo lahko uporabili pri izboru genotipov za izboljšanje ječmena v žlahtnjenju na odpornost.

Ključne besede


RT-PCR; geni za odpornost; ječmen; genska raznolikost; RAPD markerji

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2017.109.2.03

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