Pošlji prispevek (e-pošta) Preučevanje raznolikosti izražanja genov pri dveh vrstah krčnic (Hypericum spp.) pred in po cvetenju v razmerah različnega gnojenja z dušikom
Povzetek
Šentjanževka (Hypericum perforatum L.) je že od nekdaj popularna kot tradicionalno zdravilno zelišče zaradi svojih farmacevtskih in barvilnih lastnosti. Sekundarni metabolit iz skupine naftodiantronov v vrstah iz rodu Hypericum imenovan hipericin je odgovoren za antidepresivne, antikancerogene in antivirusne lastnosti tega zelišča. Ugotovljeno je bilo, da so pri biosintezi hipericina udeleženi številni geni. Gen hyp-1 sodeluje pri tej biosintezi preko pretvorbe emodina v hipericin. Naftodiantrona (hipericin in pseudohipericin) se v šentjanževki sintetizirata po poliketidni poti presnove. V rastlinah katalizira reakcije poliketidne presnovne poti encimski kompleks poliketid sintaza (PKS). Gena HpPKS1 in HpPKS2 kodirata PKS encimski kompleks. V tej raziskavi je bila primerjana relativna ekspresija genov hyp-1, HpPKS1, in HpPKS2 v koreninah in listih vrst Hypericum perforatum in H. androsaemum L., pred in po cvetenju, pri gnojenju z ureo 24, 48 in 72 ur po zalivanju. Največja ekspresija vseh treh genov je bila opažena po cvetenju v vzorcih vrste H. perforatum, 72 ur po tem, ko je bila rastlina fertirigirana z 1 g l-1 uree (hyp-1 v koreninah; HpPKS1 in HpPK (v listih). Relativna ekspresija hyp-1 v koreninah je bila večja kot v listih, a ekspresija genov HpPKS1 in HpPKS2 v listih je bila večja kot v koreninah. Relativna ekspresija vseh treh genov je bila v vrsti H. perforatum večja kot v vrsti H. androsaemum. S povečevanjem intervala med gnojenjem z ureo in zalivanjem se je pokazal trend naraščajoče ekspresije genov, kar se je pokazalo tudi s povečevanjem odmerka uree.
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DOI: http://dx.doi.org/10.14720/aas.2020.116.1.1593
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