Neposredna in posredna in vitro regeneracija rastlin iz izsečkov nodijev in listnih pecljev pri muškatki (Pelargonium odoratissimum (L.) L Herit)

Asghar EBRAHIMZADEH, Maliheh FATHOLLAHZADEH, Mohammad Ali AAZAMI, Mohammad Bagher HASSANPOURAGHDAM

Povzetek


V raziskavi so bili uporabljeni izsečki nodijev in listnih pecljev za preučevanje neposredne in posredne vzgoje rastlin muškatke (Pelargonium odoratissimum (L.) L ̍Hér.) in vitro. Neposredna tvorba poganjkov iz izsečkov nodijev je bile preiskušena na MS gojišču, ki je vsebovalo 1 in 2 mg l-1 BAP in 0,5 mg l-1 IBA. Največje poprečno število poganjkov in največje število poganjkov na izseček je nastalo v gojišču, ki je vsebovalo 2 mg l-1 BAP. Nodiji so bili vir izsečkov listnih pecljev, ki so bili gojeni na polovičnem MS gojišču z dodatkom 1, 1.5, 2 in 4,5 mg l-1 BAP, obogatenega z 0,1, 1 in 1,5 mg l-1 NAA. Z uporabo izsečkov listnih pecljev je bil dosežen najmanjši odstotek porjavitev, najboljša indukcija kalusa in največje število poganjkov  pri dodatku 2 mg l-1 BAP + 0,1 mg l-1 NAA v gojišče. Gojišče, kateremu je bil dodano 0,2 mg l-1 NAA je pokazalo zaželjen odstotek ukoreninjenja in največje poprečno število in dolžino korenin. Vkoreninjene mlade rastline so bile posajene v lončke z mešanico šotnega mahu in perlita (1:1). Aklimatizacija je bila uspešna, saj se je več kot 90 % rastlin uspešno prilagodilo razmeram v rastlinjaku. Takšno metodologijo in vitro razmnoževanja rastlin bi bilo primerno uvesti v sisteme razmnoževanja, v katerih želimo vzgojiti homogene klonske rastline za komercialne namene in podrobnejše molekularne raziskave.


Ključne besede


indukcija kalusa; MS gojišče; nodijski izsečki; Pelargonium odoratissimum; regeneracija poganjkov

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2022.118.4.1723

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Avtorske pravice (c) 2022 Asghar EBRAHIMZADEH, Maliheh FATHOLLAHZADEH, Mohammad Ali AAZAMI, Mohammad Bagher HASSANPOURAGHDAM

 

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