Učinkovit protocol za in vitro regeneracijo nodijskih izsečkov vrste Withania coagulans (Stocks) Dunal, cenjene zdravilne rastline

Pari DEHVARI-NAGAN, Hosein ABBASPOUR, Mohammad Hasan ASARE, Sara SAADATMAND

Povzetek


Z namenom izboljšanja protokola za učinkovito mikropropagacijo pomembne zdravilne rastline (Withania coagulans (Stocks) Dunal) so bili preučevani učinki različnih koncentracij in kombinacij rastnih regulatorjev na izsečkih kolenc v dveh neodvisnih poskusih. Za razvoj poganjkov je bilo uporabljeno MS gojišče, obogateno z različnimi koncentracijami in kombinacijami IBA (0,01; 0,1 in 0,5 mg l-1), BA (0,5; 1 in 2 mg l-1), Kin (0,5 in 1 mg l-1), PG (0,5 mg l-1) in GA (0,5 mg l-1). Največji odziv v rasti poganjkov, v njihovem številu in dolžini je bil dosežen pri obravnavanju MS + IBA (0,01 mg l-1) + BA (0,5 mg l-1) + PG (0,5 mg l-1) + GA (0,5 mg l-1). V drugem poskusu je bil preučevan učinek MS z dodatkom različnih koncentracij in kombinacij IBA (0,1; 0,5; 1 in 2 mg l-1), NAA (0,1 in 1 mg l-1) in PG (1 mg l-1) na zakoreninjenje nodijskih izsečkov, pri čemer je bil dosežen največji odziv zakoreninjenja (%) pri obravnavanju MS obogatenim z NAA (0,1 mg l-1), NAA (1 mg l-1), NAA (0,1 mg l-1) + PG (1 mg l-1), in NAA (1 mg l-1) + PG (1 mg l-1). Največje število korenin je bilo pri obravnavanju s NAA (0,1 mg l-1), največja dolžina korenin pa pri obravnavanju z IBA (1 mg l-1). Izsledki raziskave pojasnjujejo celotno metodo mikropropagacije vrste W. coagulans iz izsečkov kolenc, kar je pomemben prispek k vzgoji sadilnega materiala te vrste za uporabo v zdravstvu.

Ključne besede


Withania coagulans; mikropropagacija; in vitro; nodijski izsečki; floroglucinol

Celotno besedilo:

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Literatura


Ahmed, M. B., Salahin, M., Karim, R., Razvy, M. A., Hannan, M. M., Sultana, R., Hossain, M., & Islam, R. (2007a). An efficient method for in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana Bertoni.) in Bangladesh. American-Eurasian Journal of Scientific Research, 2(2), 121-125.

Ahmed, S., Kabir, A. H., Ahmed, M. B., Razvy, M. A., & Ganesan, S. (2007b). Development of rapid micropropagation method of Aloe vera L. Sjemenarstvo, 24, 121–128.

Chen, L. X., He, H., & Qui, F. (2011). Natural withanolides: an overview. Nature Product Reports, 28, 705–740. https://doi.org/10.1039/c0np00045k

Gerami, M., Ghorbani, A., & Karimi, S. (2018). Role of salicylic acid pretreatment in alleviating cadmium-induced toxicity in Salvia officinalis L. Iranian Journal of Plant Biology, 10(1), 81-95.

Ghasemi-Omran VO, Ghorbani A, Sajjadi-Otaghsara SA (2021) Melatonin alleviates NaCl-induced damage by regulating ionic homeostasis, antioxidant system, redox homeostasis, and expression of steviol glycosides-related biosynthetic genes in in vitro cultured Stevia rebaudiana Bertoni. In Vitro Cell Dev Biol.-Plant. https://doi.org/10.1007/s11627-021-10161-9

Ghorbani, A., Ghasemi Omran, V. O., Razavi, S. M., Pirdashti, H., & Ranjbar, M. (2019). Piriformospora indica confers salinity tolerance on tomato (Lycopersicon esculentum Mill.) through amelioration of nutrient accumulation, K+/Na+ homeostasis and water status. Plant Cell Reports, 38, 1151–1163. https://doi.org/10.1007/s00299-019-02434-w

Ghorbani, A., Razavi, S. M., Ghasemi Omran, V. O., & Pirdashti, H. (2018). Piriformospora indica inoculation alleviates the adverse effect of NaCl stress on growth, gas exchange and chlorophyll fluorescence in tomato (Solanum lycopersicum L.). Plant Biology, 20(4), 729-736. https://doi.org/10.1111/plb.12717

Gilani, S. A., Kikuchi, A., & Watanabe, K. N. (2009). Genetic variation within and among fragmented populations of endangered medicinal plant, Withania coagulans (Solanaceae) from Pakistan and its implications for conservation. African Journal of Biotechnology, 8, 2948–2958.

Haq, I., Youn, U. J., Chai, X., Park, E. J., Kondratyuk, T. P., Simmons, C. J., Borris, R. P., Mirza, B., Pezzuto, J. M., & Chang, L. C., (2013). Biologically active withanolides from Withania coagulans. Journal of Natural Products, 76, 22–28. https://doi.org/10.1021/np300534x

Ibanez, M. R., & Amo-Marc, J. B. (1998). Promotion by phloroglucinol of micropropagation of Minuartia valentina, an endangered and endemic Spanish plant. Plant Growth Regulators, 26, 49-56. https://doi.org/10.1023/A:1006050122173

Jain, R., Sinha, A., Jain, D., Kachhwaha, S., & Kothari, S. L. (2010). Adevntitious shoot regeneration and in vitro biosynthesis of steroidal lactones in Withania Coagulans (Stocks) Dunal. Plant Cell, Tissue and Organ Culture, 105, 135-140 https://doi.org/10.1007/s11240-010-9840-3

Maurya, R., & Akanksha, J. (2010). Chemistry and pharmacology of Withania coagulans: an ayurvedic remedy. Journal of Pharmacy and Pharmacology, 62, 153–160. https://doi.org/10.1211/jpp.62.02.0001

Rathore, M. S., Mastan, S. G., Yadav, P., Bhatt, V. D., Shekhawat, N. S., & Chikara, J. (2016). Shoot regeneration from leaf explants of Withania coagulans (Stocks) Dunal and genetic stability evaluation of regenerates with RAPD and ISSR markers. South African Journal of Botany, 102, 12–17. https://doi.org/10.1016/j.sajb.2015.08.003

Rathore, M. S., Shekhawat, S., Kaur, G., Singh, R. P., & Shekhawat, N. S. (2012). Micropropagation of vegetable rennet (Withania coagulans [Stocks] Dunal)—a critically endangered medicinal plant. Journal of Sustainable Forestry, 31, 727–746. https://doi.org/10.1080/10549811.2012.706533

Saritha, K. V., & Naidu, C. V. (2007). In vitro flowering of Withania somnifera Dunal. -An important antitumor medicinal plant. Plant Science, 172, 847-851. https://doi.org/10.1016/j.plantsci.2006.12.016

Sivansean, I., & Murugesan, K. (2008). An efficient regeneration from nodal explants of Withania somnifera Dunal. Asian Journal of Plant Sciences, 7(6), 551- 556. https://doi.org/10.3923/ajps.2008.551.556

Valizadeh, J., & Valizadeh, M. (2009). In vitro callus induction and plant regeneration from Withania coagulans: A valuable medicinal plant. Pakistan Journal of Biological Sciences, 12(21), 1415-1419. https://doi.org/10.3923/pjbs.2009.1415.1419

Valizadeh, J., & Valizadeh, M. (2011). Development of efficient micropropagation protocol for Withania coagulans (Stocks) Dunal. African Journal of Biotechnology, 10(39), 7611-7616




DOI: http://dx.doi.org/10.14720/aas.2021.117.2.1754

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