Pošlji prispevek (e-pošta) Morfološka in mikrosatelitska analiza stare črnogorske sorte oljke ‘Žutica’ sta odkrili variabilnost klonov
Povzetek
Sorta ‘Žutica’ predstavlja najbolj pogosto zastopano črnogorsko sorto oljke, ki se uporablja za pridelavo oljčnega olja ter za vlaganje zelenih in obarvanih plodov. Tradicionalno se oljko razmnožuje vegetativno, zato je pričakovati majhen genetski polimorfizem med kloni iste sorte. To je bilo pri črnogorski sorti ‘Žutica’ le delno proučeno in je predmet te raziskave. Za določitev znotraj sortne genetske variabilnosti z analizo morfoloških lastnosti in mikrosatelitov je bilo izbranih 23 dreves. Analiza glavnih komponent (PCA) je s prvimi šestimi osmi pojasnila 91,3 % celokupne variabilnosti, pri čemer so k vrednosti prvih treh osi najbolj prispevale lastnosti ploda in endokarpa. Združevanje z metodo netehtane aritmetične sredine na osnovi dvajsetih morfoloških lastnosti je drevesa ‘Žutice’ razvrstilo v dve skupini, pet dreves pa je ostalo nerazvrščenih. Z začetnimi oligonukleotidi za devet mikrosatelitskih lokusov se je pomnožilo 31 fragmentov, od katerih je bilo 22 polimorfnih, kar je omogočilo določitev devetih mikrosatelitskih profilov (potencialno različnih klonov). Pri primerjavi skupin iz dendrogramov, izdelanih na osnovi znotraj sortne variabilnosti morfoloških in mikrosatelitskih označevalcev, je bila ugotovljena majhna vrednost kofenetske korelacije. Rezultati so pokazali, da ima stara sorta ‘Žutica’, z relativno majhnega geografskega območja, raznoliko genetsko osnovo, kar bi lahko uporabili za izbor najboljših klonov.
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DOI: http://dx.doi.org/10.14720/aas.2020.116.2.1774
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