Vpliv različnih dejavnikov na indukcijo lasastih korenin pri janežnem ožepu (agastache foeniculum (pursh) kuntze)

Elnaz NOUROZI, Bahman HOSSEINI, Abbas HASSANI

Povzetek


Bakterija Agrobacterium rhizogenes je znana kot naravno orodje genskega inženiringa pri mnogih rastlinskih vrstah. V tej raziskavi so prvič preučevali indukcijo lasastih korenin pri janežnem ožepu (Agastache foeniculum (Pursh.) Kuntze) z uporabo bakterije A. rhizogenes, vsebnost rožmarinske kisline in vpliv različnih gojišč ter inokulacijskih metod na rast lasastih korenin. Kulturo lasastih korenin janežnega ožepa so vzgojili iz izsečkov enomesečnih listov inokuliranih z bakterijo A. rhizogenes sev A4 z uporabo dveh inokulacijskih metod – potapljanje in vbrizgavanje, v razmerah na svetlem in v temi. Pri metodi potapljanja izsečkov v bakterijsko suspenzijo so preučevali tudi vpliv časa (3, 5 in 7 min) na indukcijo korenin. V drugem delu raziskave so preučevali vpliv različnih osnovnih gojišč (MS, 1/2 MS in B5) na kulturo lasastih korenin. Vsebnost rožmarinske kisline v transformiranih lasastih koreninah in netransformiranih koreninah so analizirali s HPLC metodo. Značilna razlika v indukciji lasastih korenin v odvisnosti od metod inokulacije ni bila ugotovljena. Opazovanja so pokazala, da je bil odstotek indukcije lasastih korenin večji, če so izsečke listov potopili v bakterijsko suspenzijo za pet minut. Indukcija lasastih korenin je bila največja v razmerah gojitve na svetlobi v primerjavi z gojenjem v temi. Različna gojišča so se razlikovala v vrsti in količini hranil in so vplivala na rast korenin. Največja rast lasastih korenin (1.61 g sveže mase/50 ml) je bila dobljena pri polovični koncentraciji osnovnega gojišča (1/2 MS). Vsebnost rožmarinske kisline je bila v transformiranih koreninah značilno večja (213.42 µg/g suhe mase) kot v netransformiranih (52.28 µg/ g suhe mase).

Ključne besede


Agastache foeniculum, Agrobacterium rhizogenes, gojišče, lasaste korenine, potapljanje, vbrizgavanje, rožmarinska kislina

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DOI: http://dx.doi.org/10.14720/aas.2016.107.1.05

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