Vpliv dodajanja poliaminov na antioksidativno obrambo in produkcijo eteričnih olj pri zdravilni špajki (Valeriana officinalis L.) v razmerah sušnega stresa

Seyed Hamid MUSTAFAVI, Fariborz SHEKARI, Hamid Hatami MALEKI

Povzetek


Namen raziskave je bil določiti učinke foliarnega dodajanja poliaminov (PAs) na antioksidacijsko obrambo in tvorbo eteričnih olj pri zdravilni špajki (Valeriana officinalis L.) gojeni pri različni oskrbi z vodo (100, 70, 50 in 30 % vsebnosti razpoložljive vode). Raziskava je bile izvedena kot lončni poskus v rastlinjaku. Sadike špajke v sušnem stresu so bile škropljene z 1 mM raztopino putrescina (Put), spermidina (Spd) in spermina (Spm). Rezultati so pokazali, da je sušni stres značilno vplival na večino analiziranih biokemičnih parametrov v zdravilni špajki. Parametri kot so relativna vsebnost vode, vsebnost klorofila a in b so se zmanjšali, medtem ko sta se vsebnost karotenoidov in iztok elektrolitov povečala z večanjem sušnega stresa. Obrambni mehanizmi rastline, ki obsegajo vsebnost prolina, topne sladkorje, aktivnost katalaze in askorbat peroksidaze so se z večanjem sušnega stresa povečali, da bi omilili škodljive učinke stresa. Rezultati so pokazali, da je foliarno dodajanje Spd in Spm sprožilo antioksidacijsko aktivnost encimov in akumulacijo prolina v zdravilni špajki, kar je zmanjšalo poškodbe membrane. Posledično sta Spd in Spm povečala vsebnost fotosinteznih barvil, ki rastlini omogočajo oskrbo z energijo za rast in produkcijo. Dodajanje putrescina je imelo negativen učinek na aktivnost katalaze (CAT) in vsebnost klorofila a. Pozitivni učinki dodajanja poliaminov so bili opazni le v razmerah zmernega sušnega stresa in so dobili nasproten trend ob njegovi zaostritvi. Z vidika kakovosti in količine pridelka je sušni stres zmanjšal rast korenin, a povečal vsebnost eteričnih olj. Poliamini lahko oblažijo zmanjšano rast korenin, ki jo povzroča pomanjkanje vode. Ti rezultati nakazujejo, da lahko pridelovalci v razmerah zmernega sušnega stresa z dodatkom poliaminov povečajo pridelek zdravilne špajke.

Ključne besede


biokemični parametri, eterična olja, poliamini, Valeriana officinalis, sušni stress

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2016.107.1.09

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