Solni in sušni stres se izražata kot oksidacijski stres in vplivata na vzorce beljakovin v koreninah in listih dateljeve palme (Phoenix dactylifera L.)
Povzetek
Tvorba novih beljakovin je pod vplivom neugodnih okoljskih razmer prilagoditveni odziv rastlin. Dvoletne dateljeve palme, vzgojene v tkivnih kulturah iz sorte ‘Barhee’, posajene na prostem, so bile izpostavljene solnemu stresu (70 g l-1 NaCl) in dehidraciji z uporabo 70 g l-1 polietilen glikola ali brez zalivanja za en mesec (0 g l-1). Vsebnost topnih ogljikovih hidratov se je v listih povečala kot odziv na slanost in obravnavanje s polietilen glikolom v primerjavi s kontrolo in obravnavanjem s sušo brez zalivanja. Vsebnost prolina se je povečala v vseh obravnavanjih. Vsebnosti malondialdehida in vodikovega peroksida sta se povečali v razmerah slanosti. Obravnavanje s slanostjo je povečalo aktivnosti encimov askorbat peroksidaze in katalaze. Obravnavanji s slanostjo in polietilen glikolom sta povečali vsebnost abscizinske kisline, a zmanšali vsebnost indolocetne kisline. Vorec beljakovin v koreninah in listih določen z enodimenzionalno poliakrilamidno gelsko elektroforezo je pokazal, da so stresne razmere vodile k novemu vzorcu beljakovin v stresnih razmerah in izginjanju drugih. Primerjava vzorcev beljakovin med kontrolo in stresnimi obravnavanji je pokazala, da je relativna jakost pojavljanja beljakovin v koreninah in listih bolj povezana z obravnavanjem s slanostjo kot s sušo. Rezultati bi lahko bili pomembni pri pojasnitvi molekularnega mehanizma tolerance pod vplivom ekstremnega okoljskega stresa.
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DOI: http://dx.doi.org/10.14720/aas.2021.117.1.1829
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