Odkritje in molekularna karakterizacija prvega ambidensovirusa pri čebelah

Sabina OTT RUTAR, Dušan KORDIŠ

Povzetek


Čebele igrajo ključno vlogo v svetovni proizvodnji hrane kot opraševalci številnih poljščin. Številni stresorji povzročajo upad populacij gojenih in divjih vrst čebel, kot so degradacija habitata, izpostavljenost pesticidom in patogeni. Virusi delujejo kot glavni stresorji in lahko okužijo številne vrste. Večina virusov, ki okužijo čebele, so RNA virusi iz reda Picornavirales. Čeprav so nekateri ssDNA virusi pogosti pri žuželkah, na primer densovirusi, jih pri čebelah doslej še niso našli. Densovirusi pa so bili najdeni pri čmrljih in mravljah. Pokazali smo, da so densovirusi prisotni v transkriptomu azijskih čebel (Apis cerana) z južne Kitajske. Na osnovi nestrukturnih in strukturnih transkriptov smo ugotovili genomsko strukturo Apis densovirusa. Filogenetska analiza je pokazala, da novi Apis densovirus spada v rod Scindoambidensovirus v poddružini Densovirinae. Apis densovirus ima ambisense organizacijo genoma in kodira tri nestrukturne proteine in razcepljeni VP (kapsidni) protein. Dostopnost skoraj celotnega genoma Apis densovirusa bo omogočila analizo njihovega potencialno patogenega vpliva na čebele. Naše ugotovitve lahko privedejo do nadaljnjih raziskav densovirusov pri čebelah in čmrljih.

Ključne besede


čebele; densovirus; organizacija genoma; molekulska karakterizacija

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2020.116.2.1832

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