Variabilnost izolatov virusa nekrotične obročkaste pegavosti breskve (PNRSV) in virusa pritlikavosti slive (PDW) na višnji in češnji v Ukrajini

Liliia PAVLIUK, Kateryna UDOVYCHENKO, Iryna RIABA, Mykola BUBLYK

Povzetek


Virus nekrotične obročkaste pegavosti breskve (PNRSV) in virus pritlikavosti slive (PDW) sta najbolj pogosta patogena v sadovnjakih koščičastega sadja. Bolezni se z lahkoto prenašata s cvetnim prahom in se tako hitro širita v sadovnjakih, kar vodi k slabitvi dreves, povečuje njihovo občitljivost na abiotske stresorje in včasih k znatni izgubi pridelka. V Ukrajini so bile do sedaj izvedene le raziskave o razširjenosti teh virusov, ni pa bilo njihovih filogentskih raziskav.V tej raziskavi so bili z ELISA testom analizirani izolati iz vzorčenih rastlin, pozitivnih na PNRSV in PDV. Del zaporedij virusovega CP gena je bilo namnoženih in sekvenciranih za njihovo kasnejšo filogenetsko analizo. Ugotovljeno je bilo, da izolati PNRSV virusov iz Ukrajine, analizirani v tej raziskavi, pripadajo različnim skupinam in sicer skupinama PV-96 (MT828889) in PV-32 (MT892676) z največjo stopnjo istovetnosti 100 % z znanimi izolati iz NCBI GenBank. PDV izolata (MT828888 in MT828887) sta pokazala veliko medsebojno istovetnost (99.6 %), in s slovaškim izolatom iz češnje, ki se je izkazal najbolj soreden z njima z istovetnostjo 95.3 %.


Ključne besede


PNRV; PDV; ELISA; RT-PCR; češnjev virus; filogenetska analiza

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2021.117.1.1844

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