Molekulska določitev fitoplazem najdenih v nekaterih plevelih v provinci zahodni Azarbejdžan, Iran

Samaneh ZIBADOOST, Mina RASTGOU

Povzetek


Med pregledom polj, v letih 2013 in 2014, je 14 plevelnih vrst kazalo simptome okužbe s fitoplazmami kot so rumenenje in čarovniške metle. Nabrane vzorce smo analizirali z verižno reakcijo s polimerazo (PCR) z uporabo splošnih začetnih oligonukleotidov za 16SrRNA, začenši s pari začetnih oligonukleotidov P1/P7 v prvem krogu PCR analize, ki ji je sledila vgnezdena PCR anliza s parom začetnih oligonukleotidov R16F2n/R16R2. Odkrite fitoplazme so bile okarakterizirane in razlikovane s sekvenčno analizo PCR pomnožene rDNA in virtualnim polimorfizmom dolžin restrikcijskih fragmentov (RFLP). Fitoplazme, ki smo jih odkrili v simptomatični kanadski suholetnici (Conyza canadensis (L.) Cronq.), pravem brošču (Rubia tinctorum L.), divjem sirku (Sorghum halepense (L.) Pers.) in korenasti sofori (Sophora alopecuroides L.) so bile z izdelavo filogenetskega drevesa določene kot pripadnice skupine, ki povzroča proliferacijo detelje (16SrVI skupina). Nadaljnja analiza z virtualno klasifikacijo RFLP je fitoplazmi v kandski suholetnici in divjem sirku uvrstila v podskupino 16SrVI-A in fitoplazmi v pravem brošču in korenasti sofori v podskupino 16SrVI-D. To je prvo poročilo o pojavljanju od fitoplazem povzročenih bolezni na plevelih v zahodnem Azarbeidžanu, Iranu.

Ključne besede


pravi brošč, korenasta sophora, divji sirek, kanadska suholetnica, vgnezdena-PCR, Urmia

Celotno besedilo:

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Literatura


Allahverdi T, Rahimian H, Babaeizad V. 2014. First report of a Candidatus phytoplasma solani isolate affecting Sophora alopecuroides in Iran. New Disease Reports, 30: 22. DOI: 10.5197/j.2044-0588.2014.030.022

Arocha-Rosete Y, Jones P. 2010. Phytoplasma diseases of the Gramineae. In: Phyllis GW, Jones P. (eds) Phytoplasma, genomes, plant host and vectors. London, UK. 170-187.

Babaie G, Khatabi B, Bayat H, Rastgou M, Hosseini A, Salekdeh GH. 2007. Detection and characterization of phytoplasma infecting of ornamental and weed plants in Iran. Journal of Phytopathology, 155, 6: 368–372. DOI: 10.1111/j.1439-0434.2007.01247.x

Bertaccini A, Duduk B, Paltrinieri S, Contaldo N. 2014. Phytoplasmas and phytoplasma diseases: a severe threat to agriculture. American Journal of Plant Science, 5: 1763-1788. DOI: 10.4236/ajps.2014.512191

Bertaccini A, Bellardi MG, Vibio M. 1996. Virus diseases of ornamental shrubs. X. Euphorbia pulcherrima Willd. infected by viruses and phytoplasmas. Phytopathologia Mediterranea, 35: 129-132.

Chen XF, Liang YC, Chen N, Su WM, Xiao H, Wang X, Zhu XP. 2013. Molecular identification of a phytoplasma associated with Sophora root yellows. Forest Pathology, 43: 415–421. DOI: 10.1111/efp.12048

Duduk B, Tian J, Contaldo N, Fan X, Paltrinieri S, Chen Q, Zhao Q, Bertaccini A. 2010. Occurrence of phytoplasmas related to stolbur and to 'Candidatus Phytoplasma japonicum' in woody host plants in China. Journal of Phytopathology, 158: 100-104. DOI: 10.1111/j.1439-0434.2009.01586.x

Lee IM, Gundersen DE, Bertaccini A. 1998. Phytoplasma: ecology and genomic diversity. Phytopathology, 88: 1359-1366. DOI: 10.1094/PHYTO.1998.88.12.1359

Lee IM, Davis RE, Gundersen DE. 2000. Phytoplasma: phytopathogenic mollicutes. Annual Review of Microbiology, 54: 221-255. DOI: 10.1146/annurev.micro.54.1.221

Lee IM, Hammond RW, Davis RE, Gundersen DE. 1993. Universal amplification and analysis of pathogen 16SrDNA for classification and identification of mycoplasma-like organisms. Phytopathology 83: 834–842. DOI: 10.1094/Phyto-83-834

Li CL, Du YJ, Xiang BC. 2013. Molecular detection and identification of Sophora alopecuroides witches' broom phytoplasma. Xinjiang Agricultural Science, 50: 92-99.

Marcone C, Ragozzino A, Seemuller E. 1997. Detection and identification of phytoplasmas infecting vegetable, ornamental and foliage crops in southern Italy. Journal of Plant Pathology, 79: 211-217.

Salehi M, Izadpanah K, Nejat N. 2006. A new phytoplasma infecting lettuce in Iran. Plant Disease, 90: 247. DOI: 10.1094/PD-90-0247C

Schneider B, Seemuller E, Smart CD, Kirkpatrik BE. 1995. Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In: Razin S, Tully JG (eds.). Molecular and Diagnostic Procedures in Mycoplasmology, 369-380. :DOI 10.1016/b978-012583805-4/50040-6

Seemuller E, Marcone C, Lauer U, Ragozzino A, Goschl M. 1998. Current status of molecular classification of the phytoplasmas. Plant Pathology, 80: 3-26

Shumbhrata RM. 2004. Mulicutes and Plant Diseases. New Delhi. Discovery Publishing House.

Singh N, Upadhyaya PP. 2013. Analysis of medicine weeds associated with phytoplasma by morphological characters. International Journal of Medicinal Plants and Alternative Medicine, 2,1: 001-004.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, Evolutionary distance, and maximum parsimony methods. Molecular Biology Evolution, 28: 2731-2739. DOI: 10.1093/molbev/msr121

Thereza S, Baross L. 2002. Erigeron witches' broom in Brazil represents new subgroup VII. The Ash yellows phytoplasma group. Phytopathology, 86: 1142-1148.

Yu ZC, Cao Y, Zhang Q, Deng DF, Liu ZY. 2012. 'Candidatus Phytoplasma ziziphi' associated with Sophora japonica witches’ broom disease in China. Journal of General Plant Pathology, 78: 298-300. DOI: 10.1007/s10327-012-0385-7

Vali Sichani F, Bahar M, Zirak L. 2014. Characterization of phytoplasma related to aster yellows group infecting annual plants in Iran, based on the studies of 16S rRNA and rp genes. Journal of Plant Protection Research, 54, 1: 1-8. DOI: 10.2478/jppr-2014-0001

Zhang Y, Uyemoto JK, Kirkpatrick BC. 1998. A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. Journal of Virological Methods, 71: 45-50. DOI: 10.1016/S0166-0934(97)00190-0

Zhao Y, Wei W, Lee IM, Shao J, Suo X, Davis RE. 2009. Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology, 59: 2582–2593. DOI: 10.1099/ijs.0.010249-0




DOI: http://dx.doi.org/10.14720/aas.2016.107.1.13

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