Pošlji prispevek (e-pošta) Vpliv nanodelcev srebra (Ag) na fiziološke in biokemične lastnosti kalusa dveh vrst materine dušice (Thymus sp.) in vrste Zataria multiflora Boiss.
Povzetek
Vrste iz rodu materine dušice (Thymus sp.) imajo velik pomen v prozivodnji hrane in zdravil. V raziskavi je bil preučevan potencialni učinek nanodelcev srebra kot eliciatorja na rast kalusa, tvorbo karvakrola in timola pri vrsti Zataria multiflora in treh vrstah materine dušice. Najprej je bil na Murashige in Skoog (MS) gojišču, ki je vsebovalo 2 mg l-1 2,4-D in 1 mg l-1 kinetina, vzgojen kalus. Potem je bil preučevan učinek dveh različnih koncentracij srebrovih nanodelcev (4 in 8 mg l-1) na rast kalusa in in tvorbo sekundarnih metabolitov. Rezultati, pridobljeni z visokotlačno tekočinsko kromatografijo (HPLC) so pokazali, da je bila po uporabi 8 mg l-1 srebrovih nanodelcev kot iliciatorjev dosežena značilno največja rast kalusa (CGR) (0,02 mm dan-1), največja sveža masa kalusa (CM) (0,99 g) in največja vsebnost karvakrola (0,68 mg l-1) in timola (11,09 mg l-1). V primerjavi različnih vrst materine dušice je bila dosežena največja vsebnost karvakrola in timola pri vrstah T. kotschyanus Boiss. & Hohen in T. daenesis Čelak pri koncentraciji srebrovih nanodelcev 8 mg l-1. Očitno je, da je stimulacijski učinek nanodelcev odvisen od doze. Izsledke raziskave bi lahko komercialno uporabili za povečanje tvorbe zdravilnih spojin pri različlnih vrstah materine dušice.
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DOI: http://dx.doi.org/10.14720/aas.2022.118.3.1873
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