Analiza vzorcev gliadinov in raznolikosti akcesij poljske pšenice (Triticum polonicum L.) v Etiopiji

Eleni SHIFERAW

Povzetek


V raziskavi so bili s kislo poliakrilamidno gelsko elektroforezo (A-PAGE) analizirani gliadini 25 akcesij poljske pšenice v 350 vzorcih semen z namenom določiti vzorce gliadinskih prog in ugotoviti obseg raznolikosti te pšenice v Etiopiji. Določeno je bilo 70 polimorfnih prog in 68 različnih vzorcev. Osemnajst različnih mobilnih prog in 16 vzorcev je bilo določenih v območju ω-gliadinov, 22 prog in 20 vzorcev v območju γ-gliadinov, 12 prog in 22 vzorcev v območju β-gliadinov in 18 prog in 10 vzorcev v območju α-gliadinov. Poprečna genetska raznolikost, izračunana na osnovi podatkov analiziranih vzorcev za ta štiri območja gliadinov je bila 0,15. Območje γ je imelo največjo raznolikost (H = 0,193), sledili sta mu območji ω (H = 0,177) in β (H = 0,168), najmanjša raznolikost je bila ugotovljena v območju α (H = 0,127). Analiza grozdov na osnovi genetske oddaljenosti je združila analizirane akcesije v sedem glavnih skupin. Čeprav je raven raznolikosti nekoliko manjša kot pri drugih tetraploidnih vrstah pšenice v Etiopiji, so odkritja pokazala obstoj spremenljivosti v zbirkah, ki bi jih lahko uporabili pri izboljšanju pšenice.


Ključne besede


A-PAGE; genetska raznolikost; gliadin; Triticum polonicum

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2021.117.1.1935

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