Subklinični mastitis pri mlečnih kamelah v Alžiriji: primerjava presejalnih testov

Leyla HADEF, Hebib AGGAD, Brahim HAMAD, Mohamed Said MAHMOUD, Aicha ADAIKA

Povzetek


Namen te študije je bil določiti mejne vrednosti in preveriti učinkovitost štirih posrednih testov za diagnozo subkliničnega mastitisa pri kamelah v primerjavi z bakteriološko kulturo. Sto triinpetdeset vzorcev mleka 17 kamel v laktaciji smo analizirali s kultivacijsko metodo, kjer je bilo 84 vzorcev mleka pozitivnih, 47 je bilo negativnih, 22 vzorcev pa je bilo okuženih. Na skupno 131 vzorcih mleka smo izmerili pH vrednost, električno prevodnost (EC) in število somatskih celic (SCC) ter opravili Kalifornijski test za mastitis (CMT). Dobro kombinacijo občutljivosti in specifičnosti smo dobili pri mejnih vrednostih: za pH 6,55, električno prevodnost 7,2 mS/cm, pozitivnim izidom CMT (+) in 240.000 somatskimi celicami na ml mleka. Občutljivost testa za SCC je bila 72,61, za pH 66,66, za EC 47,61 in za CMT 39,28 %. Specifičnost testov se je gibala med 38,02 in 72,34 %; natančnost med 51,14 in 71,75 % in pozitivna napovedna vrednost med 47,61 in 81,33 %. SCC je bilo statistično značilno povezano z bakteriološko kulturo (r = 0,415, p < 0,05). Kapa vrednost za SCC je bila višja kot pri drugih testih (SCC > CMT > ES > pH). Rezultati kažejo, da je v praktičnih pogojih reje SCC med primerjanimi metodami najnatančnejša in najbolj zanesljiva metoda za določanje subkliničnega mastitisa pri kamelah v primerjavi z bakteriološko metodo osamitve povzročiteljev mastitisa.

Ključne besede


enogrbe kamele; Camelus dromedaries; laktacija; subklinični mastitis; presejalni testi; posredni testi; pH vrednost; električna prevodnost; Kalifornijski test za mastitis; število somatskih celic; bakteriološki test

Celotno besedilo:

PDF (English)

Literatura


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DOI: http://dx.doi.org/10.14720/aas.2016.108.2.3

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Avtorske pravice (c) 2016 Leyla HADEF, Hebib AGGAD, Brahim HAMAD, Mohamed Said MAHMOUD, Aicha ADAIKA

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