Analiza genetske raznolikosti izbranih akcesij sladkornega trsa (Saccharum officinarum L.) z uporabo označevalcev na osnovi enostavnih ponavljajočih se zaporedij (ISSR)

Faith Ewewluegim EMEGHA, David Adedayo ANIMASAUN, Folusho BANKOLE, Gbadebo OLAOYE

Povzetek


Informacija o genetski raznolikosti znotraj populacij je pomembna za uporabo heterozigotičnosti za izboljšanje gojenih rastlin v žlahtniteljskih programih. Ta raziskava je bila narejena za oceno genetske raznolikosti in vzpostavitev molekularnih povezav med 20 izbranimi ekzotičnimi akcesijami sladkornega trsa na Inštitutu za preučevanje genetskih resursov sladkornega trsa v Unilorinu (Sugar Research Institute germplasm) z uporabo molekularnih markerjev na osnovi enostavno ponavljajočih se zaporedij. Genomska DNK je bila ekstrahirana iz listov sladkornega trsa s pomočjo mini kita (DNeasy Mini Kit,Qiagen). Namnožitev fragmentov je bila izvedena s polimerazno verižno reakcijo (PCR) z ISSR označevalci, pridobljeni podatki so bili analizirani s programom MEGA 4. Analiza elektroferogramov je pokazala, da je celokupno število 39 lokusov sestavljalo 369 trakov, od katerih je bilo 95,8 % polimorfnih. Biplot analiza je pokazala, da so vsi označevalci prispevali k opaženi raznolikosti, z najmanjšim deležem označevalca ISSR6. Analiza glavnih komponent je združila akcesije v štiri skupine, ki so bile mešanica vzorcev iz vseh šestih vzorčenih mest. Polimorfizem, ugotovljen v tej raziskavi je pokazal, da so vsi ISSR označevalci učinkoviti za ugotavljanje genetske raznolikosti akcesij sladkornega trsa ne glede na njihovo mesto izvora in načina gojenja.

Ključne besede


dendrogram; genetska raznolikost; genetski viri; ISSR označevalci; sladkorni trs

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2021.117.4.2015

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