Antioksidativna obramba in vsebnost sekundarnih metabo-litov v navadnem ožepku (Hyssopus officinalis L.) v razmerah solnega stresa

Zhaleh SOHEILIKHAH, Nasser KARIMI, Masoud MODARRESI, Seyed Yahya SALEHI-LISAR, Ali MOVAFEGHI

Povzetek


Solni stres je eden izmed dejavnikov, ki najbolj omejujeje rast rastlin, v razmerah zasoljenih tal je prizadeta tudi kakovost zdravilnih rastlin. Navadni ožepek (Hyssopus officinalis L.) je bil gojen v mešanici perlita in peska in zalivans Hoaglandovo hranilno raztopino, ki je vsebovala 0, 50, 100, 150, and 200 mM NaCl. Rast rastlin se je s solnim stresom zmanjšala, a relativna vsebnost vode v listih ni bila prizadeta in vsebnost klorofila se je zmanjšala le pri največji koncentraciji (200 mM NaCl). Natrij se je v rastlinah kopičil v majhnih količinah, kar nakazuje sposobnost te vrste, da izloča sol. Vsebnost topnih sladkorjev in prolina se je povečala za 1,6 ,oziroma 4,5 krat. Aktivnost antioksidacijskih encimov (peroksidaze, katalaze, askorbat peroksidaze) se je povečala po obravnavanjih s soljo, še posebej v listih. V razmerah solnega stresa se je povečala raven sekundarnih metabolitov (saponinov, fenolov, flavonoidov, antocianinov in iridoidov), celokupna antioksidacijska sposobnost alkoholnega ekstrakta listov in korenin je bila značilno večja pri rastlinah izpostavljenih soli kot pri kontroli. Rezultati so pokazali, da je navadni ožepek na sol strpna rastlinain, da se kakovost te zdravilne rastline izboljša, če jo gojimo v razmerah slanosti. 

Ključne besede


slanost; navadni ožepek; Hyssopus officinalis; sekundarni metaboliti; antioksidacijski encimi

Celotno besedilo:

PDF (English)

Literatura


Acosta-Motos, J. R., Ortuno, M. F., Bernal-Vicente, A., Diaz-Vivancos, P., Sanchez-Blanco, M. J., & Hernandez, J. A. (2017). Plant responses to salt stress: adaptive mechanisms. Agronomy, 7(1), 18. https://doi.org/10.3390/agronomy7010018

Ahl, S. A., & Omer, E. (2011). Medicinal and aromatic plants production under salt stress. A review. Herba Polonica, 57(1), 72–87.

Ahmad, P., & Sharma, S. )2008(. Salt stress and phyto-biochemical responses of plants. Plant and Soil Environment, 54, 89–99. https://doi.org/10.17221/2774-PSE

Akyol, T.Y., Yilmaz, O., Uzilday, B., Uzilday, R. Ö., & Türkan, İ. (2020). Plant response to salinity: an analysis of ROS formation, signaling, and antioxidant defense. Turkish Journal of Botany, 44(1), 1–3. https://doi.org/10.3906/bot-1911-15

Alam, M. A., Juraimi, A. S., Rafii, M. Y., Hamid, A. A., Aslani, F., & Alam, M. Z. (2015). Effects of salinity and salinity-induced augmented bioactive compounds in purslane (Portulaca oleracea L.) for possible economical use. Food Chemistry, 169, 439–47. https://doi.org/10.1016/j.foodchem.2014.08.019

Bates, L. S., Waldren, R. P., & Teare, I. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205–207. https://doi.org/10.1007/BF00018060

Becerra-Gudiño, A., Juárez-Rosete, C. R., Bugarín-Montoya, R., & Murillo-Amador, B. (2019). Growth of Rosmarinus officinalis L. and accumulation of secondary metabolites under high salinity. Revista Bio Ciencias, 6, e567.

Beers, R. F & Sizer, I. W. (1952). A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological chemistry, 195(1), 133–140. https://doi.org/10.1016/S0021-9258(19)50881-X

Bistgani, Z. E., Hashemi, M., DaCosta, M., Craker, L., Maggi, F., & Morshedloo, M. R. (2019). Effect of salinity stress on the physiological characteristics, phenolic compounds and antioxidant activity of Thymus vulgaris L. and Thymus daenensis Celak. Industrial Crops and Products, 135, 311–320. https://doi.org/10.1016/j.indcrop.2019.04.055

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3

Chaves, M. M., Flexas, J., & Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany, 103(4), 551–560. https://doi.org/10.1093/aob/mcn125

Cheng, Y., Song, C. (2006). Hydrogen peroxide homeostatis and signaling in plant cells. Science in China, Series C (Life Sciences-English Edition), 49(1), 1.

Dazy, M., Béraud, E., Cotelle, S., Meux, E., Masfaraud, J.F., & Férard J.F. (2008). Antioxidant enzyme activities as affected by trivalent and hexavalent chromium species in Fontinalis antipyretica Hedw. Chemosphere, 73(3), 281–290. https://doi.org/10.1016/j.chemosphere.2008.06.044

Dinda, B., Dinda, M., Kulsi, G., Chakraborty, A., & Dinda S. (2019). Therapeutic potentials of plant iridoids in Alzheimer’s and Parkinson’s diseases: A review. European Journal of Medicinal Chemistry, 169,185–199. https://doi.org/10.1016/j.ejmech.2019.03.009

Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3): 350–356. https://doi.org/10.1021/ac60111a017

El Aziz, M. M., Ashour, A. S., & Melad, A. S. (2019) A review on saponins from medicinal plants: chemistry, isolation, and determination. Journal of Nanomedicine Research, 8, 6–12.

Fathiazad, F., & Hamedeyazdan, S. (2011) A review on Hyssopus officinalis L.: Composition and biological activities. African Journal of Pharmacy and Pharmacology, 5(17), 1959-1966 https://doi.org/10.5897/AJPP11.527

Fathiazad, F., Mazandarani, M., & Hamedeyazdan, S. (2011) Phytochemical analysis and antioxidant activity of Hyssopus officinalis L. from Iran. Advanced Pharmaceutical Bulletin, 1(2), 63.

Flores-de-Santiago, F., Kovacs, J., Wang, J., Flores-Verdugo, F., Zhang, C., & González-Farías, F. (2016). Examining the influence of seasonality, condition, and species composition on mangrove leaf pigment contents and laboratory based spectroscopy data. Remote Sensing, 8(3), 226–246. https://doi.org/10.3390/rs8030226

Foyer, C., Descourvieres, P., & Kunert, K. (1994). Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. Plant Cell Environment, 17(5), 507–523. https://doi.org/10.1111/j.1365-3040.1994.tb00146.x

Fuchs, A., & Bowers, M. D. (2004). Patterns of iridoid glycoside production and induction in Plantago lanceolata and the importance of plant age. Journal of Chemical Ecology, 30(9), 1723–1741. https://doi.org/10.1023/B:JOEC.0000042398.13765.83

Fukumoto, L. R., & Mazza, G. (2000). Assessing antioxidant and prooxidant activities of phenolic compounds. Journal of Agricultural and Food Chemistry, 48(8), 3597–3604. https://doi.org/10.1021/jf000220w

Giusti, M. M., Wrolstad, R. E. (2001). Characterization and measurement of anthocyanins by UV‐visible spectroscopy. Current Protocols in Food Analytical Chemistry, 00(1), F1.2.1-F1.2.13. https://doi.org/10.1002/0471142913.faf0102s00

Gupta, B., & Huang B. (2014). Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. International Journal of Genomics, e701596. https://doi.org/10.1155/2014/701596

Heath, R. L., & Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125(1), 189–198. https://doi.org/10.1016/0003-9861(68)90654-1

Hiai, S., Oura, H., Odaka, Y., & Nakajima, T. (1975). A colorimetric estimation of ginseng saponins. Planta Medica, 28(8), 363–369. https://doi.org/10.1055/s-0028-1097871

Hristova, Y., Wanner, J., Jirovetz, L., Stappen, I., Iliev, I., & Gochev, V. (2015). Chemical composition and antifungal activity of essential oil of Hyssopus officinalis L. from Bulgaria against clinical isolates of Candida species. Biotechnol Biotechnol Equip, 29, 592–601. https://doi.org/10.1080/13102818.2015.1020341

Hussain, H., Green, I. R., Saleem, M., Raza, M. L., & Nazir, M. (2019). Therapeutic potential of iridoid derivatives: Patent review. Inventions, 4(2), 29. https://doi.org/10.3390/inventions4020029

Jahantigh, O., Najafi, F., Badi, H. N., Khavari-Nejad, R. A., & Sanjarian, F. (2016). Changes in antioxidant enzymes activities and proline, total phenol and anthocyanine contents in Hyssopus officinalis L. plants under salt stress. Acta Biologica Hungarica, 67(2), 195–204. https://doi.org/10.1556/018.67.2016.2.7

Kalra, Y. (1997). Handbook of reference methods for plant analysis. CRC press. https://doi.org/10.1201/9781420049398

Kazazi, H., Rezaei, K., Ghotb-Sharif, S. J., Emam-Djomeh, Z., & Yamini, Y. (2007). Supercriticial fluid extraction of flavors and fragrances from Hyssopus officinalis L. cultivated in Iran. Food Chemistry, 105(2), 805–811. https://doi.org/10.1016/j.foodchem.2007.01.059

Khazaie, H. R., Nadjafi, F., & Bannayan, M. (2008). Effect of irrigation frequency and planting density on herbage biomass and oil production of thyme (Thymus vulgaris) and hyssop (Hyssopus officinalis). Industrial Crops and Products, 27(3), 315–321. https://doi.org/10.1016/j.indcrop.2007.11.007

Kim, M. J., Hyun, J. N., Kim, J. A., Park, J. C., Kim, M. Y., Kim, J. G., Lee, S. J., Chun, S. C., Chung, I. M. (2007). Relationship between phenolic compounds, anthocyanins content and antioxidant activity in colored barley germplasm. Journal of Agricultural and Food Chemistry, 55(12), 4802–4809. https://doi.org/10.1021/jf0701943

Kotagiri, D., Beebi, S. K., Chaitanya, K. V. (2017). Secondary metabolites and the antimicrobial potential of five different Coleus species in response to salinity stress. BioRxiv, https://doi.org/10.1101/220368

Lin, C. C., & Kao, C. H. (1999). NaCl induced changes in ionically bound peroxidase activity in roots of rice seedlings. Plant and Soil, 216(1), 147–153. https://doi.org/10.1023/A:1004714506156

Lugan, R., Niogret, M. F., Leport, L., Guégan, J. P., Larher, F. R., Savouré, A., Kopka, J., Bouchereau, A. (2010). Metabolome and water homeostasis analysis of Thellungiella salsuginea suggests that dehydration tolerance is a key response to osmotic stress in this halophyte. The Plant Journal, 64(2), 215–229. https://doi.org/10.1111/j.1365-313X.2010.04323.x

Mattioli, R., Costantino, P., Trovato, M. (2009). Proline accumulation in plants: not only stress. Plant Signaling & Behavior, 4(11), 1016–1018. https://doi.org/10.4161/psb.4.11.9797

Mushtaq, Z., Faizan, S., & Gulzar, B. (2020). Salt stress, its impacts on plants and the strategies plants are employing against it: a review. Journal of Applied Biology and Biotechnology, 8, 81–91. https://doi.org/10.7324/JABB.2020.80315

Narayanan, P., & Akamanchi, K. (2003). Colorimetric estimation of total iridoid content of Picrorhiza kurrooa. Journal of Asian Natural Products Research, 5(2), 105–111. https://doi.org/10.1080/1028602021000054955

Netala, V. R., Ghosh, S. B., Bobbu, P., Anitha, D., & Tartte, V. (2015). Triterpenoid saponins: a review on biosynthesis, applications and mechanism of their action. International Journal of Pharmacy and Pharmaceutical Sciences, 7(1), 24–28.

Oke, F., Aslim, B., Ozturk, S., & Altundag, S. (2009). Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten. Food Chemistry, 112(4), 874–879. https://doi.org/10.1016/j.foodchem.2008.06.061

Paiva, E. P., Torres, S. B., Alves, T. R, Sá, F. V., Leite, M. D., & Dombroski, J. L. (2018). Germination and biochemical components of Salvia hispanica L. seeds at different salinity levels and temperatures. Acta Scientiarum. Agronomy, 40, e39396. https://doi.org/10.4025/actasciagron.v40i1.39396

Parida, A.K., & Das A.B. (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60(3), 324–349. https://doi.org/10.1016/j.ecoenv.2004.06.010

Parihar, P., Singh, S., Singh, R., Singh, V.P., & Prasad, S.M. (2015). Effect of salinity stress on plants and its tolerance strategies: a review. Environmental Science and Pollution Research, 22(6), 4056–4075 https://doi.org/10.1007/s11356-014-3739-1

Petropoulos, S. A., Levizou, E., Ntatsi, G., Fernandes, Â., Petrotos, K., & Akoumianakis, K., et al. (2017). Salinity effect on nutritional value, chemical composition and bioactive compounds content of Cichorium spinosum L. Food Chemistry, 214, 129–136. https://doi.org/10.1016/j.foodchem.2016.07.080

Pirbalouti, A. G., Mohamadpoor, H., Bajalan, I., & Malekpoor, F. (2019). Chemical compositions and antioxidant activity of essential oils from inflorescences of two landraces of hyssop [Hyssopus officinalis L. subsp. angustifolius (Bieb.)] cultivated in Southwestern, Iran. Journal of Essential Oil Bearing Plants, 22(4), 1074–1081. https://doi.org/10.1080/0972060X.2019.1641431

Rezaei Savadkouhi, N., Ariaii, P., & Charmchian Langerodi, M. (2020). The effect of encapsulated plant extract of hyssop (Hyssopus officinalis L.) in biopolymer nanoemulsions of Lepidium perfoliatum and Orchis mascula on controlling oxidative stability of soybean oil. Food Science & Nutrition, 8(2), 1264–1271. https://doi.org/10.1002/fsn3.1415

Rosa, M., Prado, C., Podazza, G., Interdonato, R., González, J. A., Hilal, M., & Prado, F. E. (2009). Soluble sugars: Metabolism, sensing and abiotic stress: A complex network in the life of plants. Plant Signaling & Behavior, 4(5), 388–393. https://doi.org/10.4161/psb.4.5.8294

Salachna, P., Grzeszczuk, M., Meller, E., & Mizielińska, M. (2019). Effects of gellan oligosaccharide and NaCl stress on growth, photosynthetic pigments, mineral composition, antioxidant capacity and antimicrobial activity in red perilla. Molecules, 24(21), 3925. https://doi.org/10.3390/molecules24213925

San Miguel-Chávez, R. (2017). Phenolic antioxidant capacity: A review of the state of the art. Phenolic Compounds-Biological Activity, 8, 59–74. https://doi.org/10.5772/66897

Sarkar, K., & Sil, P. C. (2006). A 43 kDa protein from the herb Cajanus indicus L. protects thioacetamide induced cytotoxicity in hepatocytes. Toxicology in vitro, 20(5), 634–640. https://doi.org/10.1016/j.tiv.2005.11.003

Seevers, P., Daly, J., & Catedral, F. (1971). The role of peroxidase isozymes in resistance to wheat stem rust disease. Plant Physiology, 48(3), 353–360. https://doi.org/10.1104/pp.48.3.353

Sergiev, I., Alexieva, V., & Karanov, E. (1997). Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Comptes Rendus de l ‘Academie Bulgare des Sciences, 51(3), 121–124.

Sethi, S., Joshi, A., Arora, B., Bhowmik, A., Sharma, R. R., & Kumar, P. (2020). Significance of FRAP, DPPH, and CUPRAC assays for antioxidant activity determination in apple fruit extracts. European Food Research and Technology, 246(3), 591–598. https://doi.org/10.1007/s00217-020-03432-z

Shahidi, F., & Ambigaipalan, P. (2015). Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects–A review. Journal of Functional Foods, 18, 820–897. https://doi.org/10.1016/j.jff.2015.06.018

Shu-Hsien, H. U., Chih-Wen, Y. U., & Lin, C. H. (2005). Hydrogen peroxide functions as a stress signal in plants. Botanical Bulletin of Academia Sinica, 46, 1–10.

Sparg, S. G., Light, M. E., Van Staden, J. (2004). Biological activities and distribution of plant saponins. Journal of ethnopharmacology, 94(2-3), 219–243. https://doi.org/10.1016/j.jep.2004.05.016

Taarit, M. B., Msaada, K., Hosni, K., Hammami, M., Kchouk, M. E., Marzouk, B. (2009). Plant growth, essential oil yield and composition of sage (Salvia officinalis L.) fruits cultivated under salt stress conditions. Industrial Crops Production, 30, 333–337.

https://doi.org/10.1016/j.indcrop.2009.06.001

Valifard, M., Mohsenzadeh, S., Kholdebarin, B., & Rowshan, V. (2014). Effects of salt stress on volatile compounds, total phenolic content and antioxidant activities of Salvia mirzayanii. South African Journal of Botany, 93, 92–97. https://doi.org/10.1016/j.sajb.2014.04.002

Verbruggen, N., & Hermans, C. (2008). Proline accumulation in plants: a review. Amino acids, 35(4), 753–759. https://doi.org/10.1007/s00726-008-0061-6

Verma, N., & Shukla, S. (2015). Impact of various factors responsible for fluctuation in plant secondary metabolites. Journal of Applied Research in Medicinal and Aromatic Plants, 2 (4), 105–113. https://doi.org/10.1016/j.jarmap.2015.09.002

Wang, C., Gong, X., Bo, A., Zhang, L., Zhang, M., Zang, E., Zhang, C., & Li, M. (2020). Iridoids: research advances in their phytochemistry, biological activities, and pharmacokinetics. Molecules, 25(2), 287. https://doi.org/10.3390/molecules25020287

Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555–559. https://doi.org/10.1016/S0308-8146(98)00102-2

Zrig, A., Tounekti, T., Hegab, M. M., Ali, S. O., & Khemira, H. (2016). Essential oils, amino acids and polyphenols changes in salt-stressed Thymus vulgaris exposed to open–field and shade enclosure. Industrial Crops and Products, 91, 223–230. https://doi.org/10.1016/j.indcrop.2016.07.012




DOI: http://dx.doi.org/10.14720/aas.2021.117.2.2065

Povratne povezave

  • Trenutno ni nobenih povratnih povezav.


Avtorske pravice (c) 2021

##submission.license.cc.by-nc-nd4.footer##

 

Acta agriculturae Slovenica je odprtodostopna revija, ki objavlja pod pogoji licence Creative Commons Priznanje avtorstva (CC BY).

                     


eISSN 1854-1941