Assessment of morphological and molecular variation in local olive (Olea europaea L.) in the Northern part of Iran

Mahnaz Nezamivand Chegini, Habibo allah Samizade Lahiji, Mohammad Ramezani Malekroudi, Mohammad Mohsenzadeh Golfazani, Ramin Seighalani

Abstract


Iran is known as one of the origins of olive in the world with many different olive cultivars, mainly in the north. Eighty eight accessions belong to 4 main olive cultivars were investigated by 21 morphological characters and 11 ISSR markers. Analyses of morphological characters revealed the existence of high genetic variability among cultivars. Based on both morphological and ISSR cluster analyses, 88 accessions were grouped in five distinct clusters. The ISSR primers produced 77 polymorphic bands. AMOVA showed significant difference in both between and within olive cultivars. The highest and lowest coefficient of Nei’s genetic distance was observed in ‘Mari’ and ‘Shengeh’ (0.105) and ‘Zard’ and ‘Rowghani’ (0.061), respectively. In both morphological and ISSR data analyses, ‘Mari’ showed the highest homogeneity. The olive cultivars were not clustered based on their geographical origin.

Keywords


genetic diversity, ISSR, PIC, polymorphism

Full Text:

PDF

References


Angiolillo, A., Mencuccini, M., & Baldoni, L. (1999). Olive (Olea europaea L.) genetic diversity assessed Amplified Fragment Length Polymorphisms. Theoretical and Applied Genetics, 98: 411-421. Doi: 10.1007/s001220051087

Bandelj, D., Jakše, J., & Javornik, B. (2002). Characterisation of olive (Olea europaea L.) cultivars by RAPD marker. Acta Horticulturae, 586: 133-135. Doi: 10.17660/ActaHortic.2002.586.20

Belaj, A., Trujillo, I., De La Rosa, R., Rallo, L., & Gimenez, M. J. (2001). Polymorphism and discrimination capacity of randomly amplified polymorphic markers in olive germplasm bank. American Society for Horticultural Science, 126: 64-71.

Belaj, A., Rallo, L., Trujillo, I., & Baldoni, L. (2004). Using RAPD and AFLP markers to distinguish individuals obtained by clonal selection of Arbequina and Manzanilla de Sevilla olive. Horticultural Science, 39: 1566-70.

Besnard, G., Berton, C., Baradat, P., Khadari, B., & Berville, A. (2001). Cultivar identification in olive (Olea europaea L.) based on RAPDs. American Society for Horticultural Science, 126: 668-675.

Cipriani, G., Marrazo, M. T., Marconi, R., Cimato, A., & Testolini, R. (2002). Microsatellite markers isolated in olive (Olea europaea L.) are suitable for individual fingerprinting and reveal polymorpgism within ancient cultivars. Theoretical and Applied Genetics, 104: 223-228. Doi: 10.1007/s001220100685

Dastkar, E., Soleimani, A., Jafary, H., & Naghavi, M. R. (2013). Genetic and morphological variation in Iranian olive (Olea europaea L.) germplasm. Crop Breeding Journal, 3(2): 99-106.

Diaz, A., De La Rosa, R., Martin, A., & Rallo, P. (2006). Development, characterization and inheritance of new microsatellites in olive (Olea europaea L.) and evaluation of their usefulness in cultivar identification and genetic relationship studies. Tree Genetics & Genomes, 2: 165- 175. Doi: 10.1007/s11295-006-0041-5

Doyle, J. J., & Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue . Focus, 12: 13-15.

Essadki, M., Ouazzani, N., Lumaret, R., & Moumni, M. (2006). ISSR variation in olive-tree cultivars from Morocco and other western countries of Mediterranean Basin. Genetic Resources and Crop Evolution, 53: 475-482. Doi: 10.1007/s10722-004-1931-8

Gemas, V. J. V., Almadanim, M. C., Tenreriro, R., Martins, A., & Fevereiro, P. (2004). Genetic diversity in the olive tree (Olea europaea L. subsp. europaea) cultivated in Portugal revealed by RAPD and ISSR markers. Genetic Resources and Crop Evolution, 51: 501-511. Doi: 10.1023/B:GRES.0000024152.16021.40

Hamrick, J. L., & Godt, M. J. W. (1989). Allozyme diversity in plant species. In: Brown, A.H.D., Clegg, M.T., Kahler, A.L., Weir, B.S. (Eds.), Plant Population Genetics, Breeding and Genetic Resources. Sinauer Associated Sunderland, MA, pp. 43-63.

Hegazi, E. S., Hegazi, A. A., Tawfic, A. A., & Sayed, H. A. (2012). Molecular characterization of local and important olive cultivars grown in Egypt using ISSR technique. Journal of Horticultural Science & Ornamental Plants, 4(2): 148-154.

Hess, J., Kadereit, J.W., & Vargas, P. (2000). The colonization history of Olea europaea L. in Macaronesia based on internal transcribed spacer 1 (ITS-1) sequences, randomly amplified polymorphic DNAs (RAPD), and intersimple sequence repeats (ISSR). Molecular Ecology, 9: 857-867. Doi: 10.1046/j.1365-294x.2000.00942.x

Hosseini- Mazinani, S. M., & Samaee, M. (2004). Multivariate analyses of intra-cultivar variation of a local olive cultivar in the Northern part of Iran using morphological traits. Acta Horticulture, 791: 65-71.

International Olive Council (IOC). (1982). Unified Qualitative Standard Applying to table olives in international trade. Madrid.

Karhu, A., Hurme, P., Karjalainen, M., Karvonen, P., Karkkainen, K., Neale, D., & Savolainen, O. (1996). Do molecular markers reflect patterns of differentiation in adaptive traits of conifers? Theoretical and genetics, 93: 215-221.

Khadari, B., Breton, C., Moutier, N., Roger, P. J., Besnard, G., Berville, A., & Dosba, F. (2003). The use of molecular markers for germplasm management in a French olive collection. Theoretical and Applied Genetics, 106: 521-529.

Koohi-dehkordi, M., Rahimmalek, M., Sayed-tabatabaei., B. E., Baninasab, B., & Mobli, M. (2006). Assessment of genetic relationships among some of the Iranian and forign olive cultivars using ISSR markers. Iranian Journal of Horticultural Science and Technology. 7(2): 93-10.

Mekuria, G. T., Collin, G. C., & Sedgley, M. (1999). Genatic variability between different accessions of some common commericial olive cultivars.The journal of Horticultural and Science Biotechnology, 74 (3): 309-314. Doi: 10.1080/14620316.1999.11511114

Mecuria, GT., Collins, G., & Sedgley, M. (2002). Genetic diversity within n isolated olive (Olea europaea L.) population in relation to feral spread. Scientia Horticulturae, 94: 91-105. Doi: 10.1016/S0304-4238(01)00375-2

Milotić, A., Setić, E., Persurić, D., Puljuha, D., Sladonja, B. and Brščić, K. 2005. Identification and characterization of autochthonous olive varieties in Istria (Croatia). Annales Ser hist nat. 15 (2): 251-256.

Noormohammadi, Z., Hosseini-Mazinani, M., Belaj, A., Trujillo, I., Rallo, L., & Sadeghizade, M. (2007). Identification and classification of main Iranian olive cultivars using microsatellite markers. Hortscince, 42(7): 1545-1550.

Noormohammadi, Z., Sheidai, M., Dehghani, A., Parvini, F., & Hosseini-Mazinani, S. M. (2012). Inter-population genetic diversity in Olea cuspidata subsp. Cuspidata revealed by SSR and ISSR markers. Acta Biologica Szegediensis, 56(2): 155-163.

Omrani-Sabbaghi, A., Shahriari, M., Falahati-Anbaran, M., Mohammaddi, S. A., Nankali, A., Mardi, M., & Ghreyazie, B. (2007). Microsatellite markers based assessment of genetic diversity in Iranian olive (Olea europaea L.) collections. Scientia Horticulturae, 112 (4): 439-447. Doi: 10.1016/j.scienta.2006.12.051

Owen, C. A., Bita, E. C., Banilas, G., Hajjar, S. E., Sellianakis, I., Hatzopoulos, P., & Kalaitzis, P. (2005). AFLP reveals structural details of genetic diversity within cultivated olive germplasm from the Eastern Mediterranean.Theoritical and Applied Genetics, 110: 1169-1176. Doi: 10.1007/s00122-004-1861-z

Pasqualone, A., Caponio, F. & Blance, A. (2001). Inter-simple sequence repeat DNA markers for identification of drupes from different Olea europaea L. cultivars. European Food Research and Technology, 213: 240-243. Doi: 10.1007/s002170100367

Peakall, R., & Smouse, P E. (2006). GENEALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Resources. Notes 5: 288-295.

Rao, R., La Mura, M., Corrado, G., Ambrosino, O., Foroni, I., Perri, E., & Pugliano, G. (2009). Molecular diversity and genetic relationships of southern Italian olive cultivars as depicted by AFLP and morphological traits. Journal of Horticultural Science & Biotechnology, 84(3): 261-266. Doi: 10.1080/14620316.2009.11512514

Rallo, P., Dorado, G., & Martin, A. (2000). Development of simple sequence repeats (SSR) in olive tree (Olea europaea L.). Theoretical and Applied Genetics, 101:984-989. Doi: 10.1007/s001220051571

Rohlf, F J. (1998). NTSYS-pc. Numerical taxonomy and multivariate analysis system. Version 2.00. Exeter Software, Setauket, N. Y.

Rotondi, A., & Magli, M. (1999).Valutazione comparative dellasensibilita a minimetermichecritiche di cultivar di olive della Romagna. Olive & Oil,1: 48-54.

Sadeghi, H. (1992). Cultivation, preservation and harvesting of olive. Publication of the Agricultural Ministry, Tehran, Iran.

Samaee, S. M., Shobbar, Z. S., Afshari, H., Hosseini-Mazinani, M., & Aheidai, M. (2003). Molecular characterization of olive germplasm in Iran by use of Random Amplified Polymorphic DNA (RAPD): Correlation with phenotypic studies. Acta Horticulture, 623: 169-175. Doi: 10.17660/ActaHortic.2003.623.18

Sanz-Cortes, F., Parfit, D. E., Romero, C., Struss, D., Liacer, G., & Badenes, M I.( 2003). Intraspecific olive diversity assessed with AFLP. Molecular Breeding, 122: 173-177. Doi: 10.1046/j.1439-0523.2003.00808.x

Sefc K. M., Lefort, F., Grando, M. S., Scott, K. D., Steinkellner, H. & Thomas, M. R. (2001). "Microsatellite markers for grapevine: A state of the art". in Roubelakis. K. A. Molecular biology and biotechnology of grapevine. Kluwer Academic Publisher. 433-444. Doi: 10.1007/978-94-017-2308-4_17

Sensi, E., Vignani, R., Scali, M., Masi, E., & Cresti, M. (2003). DNA fingerprinting and genetic relatedness among cultivated varieties of Olea europaea L. estimated by AFLP analysis. Scientia Horticulturae, 97: 378-388. Doi: 10.1016/S0304-4238(02)00163-2

Sheidai, M., H-Shahriari, Z., Noormohammadi, Z., Parisan, H., & Farahani, F. (2007). Study of genetic diversity in some olive (Olea europaea L.) cultivars by using RAPD markers. Pakistan Journal of Boilogical Science, 10 (17): 2972-2975. Doi: 10.3923/pjbs.2007.2972.2975

Terzopoulos, P.J., Kolano, B., Bebeli, P.J., Kaltsikes, P. J., & Metzidakis, I. (2005). Identification of Olea europaea L. cultivars using inter-simple sequence repeat markers. Scientia Horticulturae, 105: 45-51. Doi: 10.1016/j.scienta.2005.01.011

Wiesman, Z., Avidan, N., Lavee, S., & Quebedeaux, B. (1998). Molecular characterization of common olive varieties in Israel AND THE West Bank using randomly amplified polymorphic DNA (RAPD) markers. American Society for Horticultural Science,123: 837-841.




DOI: http://dx.doi.org/10.14720/aas.2016.107.2.12

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Mahnaz Nezamivand Chegini, Habibo allah Samizade Lahiji, Mohammad Ramezani Malekroudi, Mohammad Mohsenzadeh Golfazani, Ramin Seighalani

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Acta agriculturae Slovenica is an Open Access journal published under the terms of the Creative Commons CC BY License.

                           


eISSN 1854-1941