Morfološka in molekularna določitev ter novi podatki o razširjenosti vrste Tetrastichus miser (Nees, 1834) (Hymenoptera: Chalcidoidea: Eulophidae) v Kašmirju

Ajaz RASOOL, Bashir A. GANAI, Shreevihar SANTHOSH, Tariq AHMAD

Povzetek


Vrsta Tetrastichus miser (Nees) (Hymenoptera: Eulophidae: Tetrastichinae) je parazitoid hroščev iz podružin Curculioninae in Scolytinae, ki napadajo različne gospodarsko pomembne drevesne vrste. V raziskavi je bil parazitoid nabran s stresalnimi mrežami in aspiratorjem na posušenih himalajskih cipresah, Cedrus deodara (Roxb.) G.Don (Pinaceae), ki so bile napadene s hrošči iz rodu Scolytus. O vrsti poročajo prvič iz doline Kashmir. Določitev parazitoida je zelo pomembna za preučevanje njegovega obnašanja, ekologije, življenskega kroga in pri njegovi uporabi v različnih programih biotičnega zatiranja škodljivcev. Poleg morfološkega opisa je bila za lažjo določitev v bodočih raziskavah uporabljena molekularna analiza na osnovi podenote I citohrom C oksidaze. Filogenetska analiza z metodama Bayezinove inference (Bayesian inference, BI) in največje verjetnostni (Maximum Likelihood, ML) je pokazala, da so se izolati vrste Tetrastichus miser združevali v istem kladu, ločeno od najbližjih, ki se ujemajo s predstavniki poddružine Tetrastichinae. Ločitev vrste Tetrastichus miser in predstavnikov poddružine Tetrastichinae je bila očitna in je znašala od 0,09 do 0,10 % (v povprečju 0,05 %). Opaženega ni bilo nobenega prekrivanja med maksimalno razdaljo znotraj vrste in minimalno razdaljo med vrstami.


Ključne besede


Tetrastichus miser; Cedrus deodara; hrošč; podenota I citohrom C oksidaze; klad; morfotaksonomija; molekularna analiza

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2022.118.1.2132

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Avtorske pravice (c) 2022 Ajaz RASOOL, Bashir A. GANAI, Shreevihar SANTHOSH, Tariq AHMAD

 

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