Izolacija bakterij, ki sproščajo fosfat iz rizosfere kot nadomestek biognojilom

Thi Thuy NGUYEN, The Anh LUU, Quang Trung DO

Povzetek


V tleh se velike količine dodanega fosforja hitro spremenijo v netopne oblike tako, da ostane rastlinam raspoložljivega le okrog 0,1 %. Zaradi tega je izkoriščanje v naravi prisotnih fosfat sproščajočih mikroorganizmov nepogrešljiva aktivnost, ki omogoča njihovo uporabo in zmanjšujej dodajanje mineralnega fosforja v kmetijska tla. V raziskavi smo preverili in izolirali 7 bakterij, ki sproščajo fosfat z različnimi indeksi sproščanja od 4,2 do 226,1. Med njimi je bil najučikovitejši izolat PSB31, ki je sproščal tri kalcijev fosfat (Ca3(PO4)2 v velikosti 962 mg l-1, na osnovi molekularnih testov določen kot IMAU61039 soj bakterije iz rodu Bacillus. Ta soj bakterije je generiral nizek pH v raztopini in fosfataze, ki so vključene v mehanizem sproščanja fosforja. Nadalje je poskus v rastlinjaku pokazal, da so vsebovale sejanke paradižnika, ki so rastle v tleh inokuliranih z izolatom PSB31 večjo vsebnost fosforja in mnogo večjo biomaso kot tiste, ki so rastle v tleh brez dodatka PSB31. Izsledki nakazujejo, da bi se izolat PSB31 lahko uporabljal kot biognojilo.


Ključne besede


fosfor sproščajoče bakterije; rast rastlin vzpodbujajoče bakterije; biognojila; paradižnik; fosfataze

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2022.118.1.2262

Povratne povezave



Avtorske pravice (c) 2022 Thi Thuy NGUYEN, The Anh LUU, Quang Trung DO

 

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