Pošlji prispevek (e-pošta) Količinsko ovrednotenje apokarotenoidov v komercialnih vzorcih indijskega (kašmirskega) žafrana z analizo UV-Vis spektroskopije in HPLC
Povzetek
Žafran velja za najdražjo začimbo v svetovnem merilu. Zaradi majhne pridelave, velikega povpraševanja in visoke cene je zaradi ekonomskih koristi podvržen ponarejanju, kar povzroča zdravstvena tveganja. Najznačilnejša lastnost za določanje kakovosti žafrana je njegova sposobnost obarvanja (vsebnost krocina), ki se določa z merjenjem UV-Vis absorbcije pri 440 nm v vodnih pripravkih te začimbe. Pikrokrocin in safranal sta ostali klučni komponenti, ki se uporabljata za določanje kakovosti žafrana. V raziskavi smo preučevali kakovost tržnega žafrana pridobljena iz različnih geografskih območij Kašmirja (Indija) z določanjem vsebnosti apokarotenoidov z UV-Vis spektroskopijo, ki ji je sledila analiza z visokotlačno tekočinsko kromatografijo (HPLC), kjer smo v vzorcih žafrana določali koncentracije metabolitov kot so krocin, pikrokrocin in safranal. V raziskavi je bilo analiziranih 31 vzorcev različnega izvora. Rezultati analize z UV-Vis spektroskopijo so pokazali, da se je med 31 vzorci samo 14 uvrstilo v kvaliteto I, 9 vzorcev seje uvrstilo v kvaliteto II in 5 vzorcev v kvaliteto III, glede na ISO kategorije. Preostali 3 vzorci niso izpolnjevali ISO standardov, kar kaže, da so bili ponarejeni. Vsebnost apokarotenoidov v vzorcih se je pri analizi s HPLC značilno razlikovala, kar bi lahko bila posledica različnega sušenja, shranjevanja ali ponarejanja.
Ključne besede
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Alonso, G. L., Salinas, M. R., & Garijo, J. (1998). Method to determine the authenticity of aroma of saffron (Crocus sativus L.). Journal of Food Protection, 61(11), 1525–1528. https://doi.org/10.4315/0362-028X-61.11.1525
Azami, S., Shahriari, Z., Asgharzade, S., Farkhondeh, T., Sadeghi, M., Ahmadi, F., Vahedi, M. M., & Forouzanfar, F. (2021). Therapeutic potential of saffron (Crocus sativus L.) in ischemia stroke. Evidence-Based Complementary and Alternative Medicine, 2021, e6643950. https://doi.org/10.1155/2021/6643950
Basker, D., & Negbi, M. (1983). Uses of saffron. Economic Botany, 37(2), 228–236. https://doi.org/10.1007/BF02858789
Beiranvand, S. P., Beiranvand, N. S., Moghadam, Z. B., Birjandi, M., Azhari, S., Rezaei, E., Salehnia, A. N., & Beiranvand, S. (2016). The effect of Crocus sativus (saffron) on the severity of premenstrual syndrome. European Journal of Integrative Medicine, 8(1), 55–61. https://doi.org/10.1016/j.eujim.2015.06.003
Biancolillo, A., Foschi, M., & D’Archivio, A. A. (2020). Geographical classification of Italian saffron (Crocus sativus L.) by multi-block treatments of UV-Vis and IR spectroscopic data. Molecules, 25(10), 2332. https://doi.org/10.3390/molecules25102332
Bolhassani, A., Khavari, A., & Bathaie, S. Z. (2014). Saffron and natural carotenoids: Biochemical activities and anti-tumor effects. Biochimica et Biophysica Acta (Bba)-Reviews on Cancer, 1845(1), 20–30. https://doi.org/10.1016/j.bbcan.2013.11.001
Caballero-Ortega, H., Pereda-Miranda, R., Riverón-Negrete, L., Hernández, J.M., Medécigo-Ríos, M., Castillo-Villanueva, A. and Abdullaev, F.I. (2004). Chemical composition of saffron (Crocus sativus L.) from four countries. Acta Horticulturae, 650, 321–326. https://doi.org/10.17660/ActaHortic.2004.650.39
Delgado, M., Zalacain, A., Pardo, J., López, E., Alvarruiz, A., & Alonso, G. (2005). Influence of different drying and aging conditions on saffron constituents. Journal of Agricultural and Food Chemistry, 53, 3974–3979. https://doi.org/10.1021/jf0404748
Hadizadeh, F., Mahdavi, M., Emami, S. A., Khashayarmanesh, Z., Hassanzadeh, M., Asili, J., Seifi, M., Nassirli, H., Shariatimoghadam, A., & Noorbakhsh, R. (2007). Evaluation of iso method in saffron qualification. Acta Horticulturae, 739, 405–410. https://doi.org/10.17660/ActaHortic.2007.739.53
Haghighi, B., Feizy, J., & Kakhki, A. H. (2007). LC determination of adulterated saffron prepared by adding styles colored with some natural colorants. Chromatographia, 66(5), 325. https://doi.org/10.1365/s10337-007-0321-8
Hajimahmoodi, M., Afsharimanesh, M., Moghaddam, G., Sadeghi, N., Oveisi, M. R., Jannat, B., Pirhadi, E., Zamani Mazdeh, F., & Kanan, H. (2013). Determination of eight synthetic dyes in foodstuffs by green liquid chromatography. Food Additives & Contaminants: Part A, 30(5), 780–785. https://doi.org/10.1080/19440049.2013.774465
Hussain, A. (2005, January 28). Saffron industry in deep distress. BBC NEWS. http://news.bbc.co.uk/2/hi/south_asia/4216493.stm
ISO - ISO 3632-1 (2011). Spices—Saffron (Crocus sativus L.)—Part 1: Specification. https://www.iso.org/standard/44523.html
Kahriz, P. P. (2020). Saffron (Crocus sativus L.) cultivation in Turkey. In Saffron (pp. 33–43). Elsevier. https://doi.org/10.1016/B978-0-12-818462-2.00003-6
Kamalipour, M., & Akhondzadeh, S. (2011). Cardiovascular effects of saffron: An evidence-based review. The Journal of Tehran Heart Center, 6(2), 59.
Karimi, S., Feizy, J., Mehrjo, F., & Farrokhnia, M. (2016). Detection and quantification of food colorant adulteration in saffron sample using chemometric analysis of FT-IR spectra. RSC Advances, 6(27), 23085–23093. https://doi.org/10.1039/C5RA25983E
Khorasany, A. R., & Hosseinzadeh, H. (2016). Therapeutic effects of saffron (Crocus sativus L.) in digestive disorders: A review. Iranian Journal of Basic Medical Sciences, 19(5), 455–469.
Kumari, L., Jaiswal, P., & Tripathy, S. (2021). Various techniques useful for determination of adulterants in valuable saffron: A review. Trends in Food Science & Technology, 111. https://doi.org/10.1016/j.tifs.2021.02.061
Lowell, G. (1964). Saffron adulteration. Journal of Association of Official Agricultural Chemists, 47(3), 538. https://doi.org/10.1093/jaoac/47.3.538
Lozano, P., Castellar, M. R., Simancas, M. J., & Iborra, J. L. (1999). A quantitative high-performance liquid chromatographic method to analyse commercial saffron (Crocus sativus L.) products. Journal of Chromatography A, 830(2), 477–483. https://doi.org/10.1016/S0021-9673(98)00938-8
Maghsoodi, V., Kazemi, A., & Akhondi, E. (2012). Effect of different drying methods on saffron (Crocus sativus L) quality. Iranian journal of chemistry & chemical engineering-international english edition, 31, 85–89.
Marieschi, M., Torelli, A., & Bruni, R. (2012). Quality control of saffron (Crocus sativus L.): Development of SCAR markers for the detection of plant adulterants used as bulking agents. Journal of Agricultural and Food Chemistry, 60(44), 10998–11004. https://doi.org/10.1021/jf303106r
Mir, T. ul G., Wani, A. K., Singh, J., & Shukla, S. (2022a). Therapeutic application and toxicity associated with Crocus sativus (saffron) and its phytochemicals. Pharmacological Research - Modern Chinese Medicine, 4, 100136. https://doi.org/10.1016/j.prmcm.2022.100136
Mir, T. U. G., Malik, A. Q., Singh, J., Shukla, S., & Kumar, D. (2022b). An overview of molecularly imprinted polymers embedded with quantum dots and their implementation as an alternative approach for extraction and detection of crocin. Chemistry Select, 7(21), e202200829. https://doi.org/10.1002/slct.202200829
Mzabri, I., Addi, M., & Berrichi, A. (2019). Traditional and modern uses of Saffron (Crocus Sativus). Cosmetics, 6(4), 63. https://doi.org/10.3390/cosmetics6040063
Ordoudi, S. A., Staikidou, C., Kyriakoudi, A., & Tsimidou, M. Z. (2018). A stepwise approach for the detection of carminic acid in saffron with regard to religious food certification. Food Chemistry, 267, 410–419. https://doi.org/10.1016/j.foodchem.2017.04.096
Pandita, D. (2021). Saffron (Crocus sativus L.): Phytochemistry, therapeutic significance and omics-based biology. In Medicinal and Aromatic Plants (pp. 325–396). Elsevier. https://doi.org/10.1016/B978-0-12-819590-1.00014-8
Paredi, G., Raboni, S., Marchesani, F., Ordoudi, S. A., Tsimidou, M. Z., & Mozzarelli, A. (2016). Insight of saffron proteome by gel-electrophoresis. Molecules, 21(2), 167. https://doi.org/10.3390/molecules21020167
Patel, K., Maguigan, K., & Shoulders, B. (2019). 648: Evaluation of beta-lactam concentrations associated with extended daily dialysis. Critical Care Medicine, 47(1), 304. https://doi.org/10.1097/01.ccm.0000551400.16360.6c
Petrakis, E. A., Cagliani, L. R., Polissiou, M. G., & Consonni, R. (2015). Evaluation of saffron (Crocus sativus L.) adulteration with plant adulterants by 1H NMR metabolite fingerprinting. Food Chemistry, 173, 890–896. https://doi.org/10.1016/j.foodchem.2014.10.107
Saxena, R. B. (2010). Botany, taxonomy and cytology of Crocus sativus series. AYU (An International Quarterly Journal of Research in Ayurveda), 31(3), 374. https://doi.org/10.4103/0974-8520.77153
Sereshti, H., Poursorkh, Z., Aliakbarzadeh, G., & Zarre, S. (2018). Quality control of saffron and evaluation of potential adulteration by means of thin layer chromatography-image analysis and chemometrics methods. Food Control, 90, 48–57. https://doi.org/10.1016/j.foodcont.2018.02.026
Siddiqui, M. J., Saleh, M. S. M., Basharuddin, S. N. B. B., Zamri, S. H. B., Mohd Najib, M. H. bin, Che Ibrahim, M. Z. bin, binti Mohd Noor, N. A., Binti Mazha, H. N., Mohd Hassan, N., & Khatib, A. (2018). Saffron (Crocus sativus L.): As an antidepressant. Journal of Pharmacy & Bioallied Sciences, 10(4), 173–180. https://doi.org/10.4103/JPBS.JPBS_83_18
Taherzadeh, Z., Khaluyan, H., Iranshahy, M., Rezaeitalab, F., Ghalibaf, M. H. E., & Javadi, B. (2020). Evaluation of sedative effects of an intranasal dosage form containing saffron, lettuce seeds and sweet violet in primary chronic insomnia: A randomized, double-dummy, double-blind placebo controlled clinical trial. Journal of Ethnopharmacology, 262, 113-116.
Zalacain, A., Ordoudi, S. A., Blázquez, I., Díaz-Plaza, E. M., Carmona, M., Tsimidou, M. Z., & Alonso, G. L. (2005). Screening method for the detection of artificial colours in saffron using derivative UV-Vis spectrometry after precipitation of crocetin. Food Additives & Contaminants, 22(7), 607–615. https://doi.org/10.1080/02652030500150051
Zilaee, M., Hosseini, S. A., Jafarirad, S., Abolnezhadian, F., Cheraghian, B., Namjoyan, F., & Ghadiri, A. (2019). An evaluation of the effects of saffron supplementation on the asthma clinical symptoms and asthma severity in patients with mild and moderate persistent allergic asthma: A double-blind, randomized placebo-controlled trial. Respiratory Research, 20(1), 39. https://doi.org/10.1186/s12931-019-0998-x
Zougagh, M., Ríos, A., & Valcárcel, M. (2005). An automated screening method for the fast, simple discrimination between natural and artificial colorants in commercial saffron products. Analytica Chimica Acta, 535(1), 133–138. https://doi.org/10.1016/j.aca.2004.11.060
DOI: http://dx.doi.org/10.14720/aas.2022.118.3.2333
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