Pošlji prispevek (e-pošta) Genetska karakterizacija lokalnih sort koruze (Zea mays L.) gojenih na Kosovu ovrednotena z MITE-Hbr označevalci
Povzetek
Namen raziskave je bil preučiti in opisati genetsko strukturo avtohtone dednine trdinke iz različnih lokalitet Kosova s Hbr označevalci. Genetska karakterizacija 6-8 sejank od vsake lokalne sorte je bila izvedena s prikazom nabora Hbr profilov, izračunanega na osnovi selektivne baze. Najučinkovitejši določitelj genetske raznovrstnosti za razločevanje med lokalnimi sortami je bila kombinacija primerjev Hbr-Int5-F/MseI+T. Najmočnejšo genetsko povezanost med genotipi (r = 55.57) je imela lokalna sorta ACC4 z oranžnimi zrni, ki je izkazovala največjo genetsko izenačenost v primerjavi z drugimi akcesijami. Klasterska analiza z uporabo modela aposteriorne verjetnosti (Bayesian approach) je za vključene lokalne sorte oblikovala dve genetski skupini. Kot merilo za analizo populacijske structure, na katero vplivata genetski zdrs in migracija, so bile izračunane vrednosti Fst za obe genetski skupini. Večja vrednost Fst (0,4027) je bila izračunana znotraj prve genetske skupine v primerjavi z drugo (0,2001), kar kaže na večji delež navskrižnega križanja in ohranjanja raznolikosti med lokalnimi sortami prve genetske skupine grozda. Podobna porazdelitev genetskih povezav je bila določena na dendrogramu, izdelanem z uporabo Dice-ovega koeficienta podobnosti in algoritma razvrščanja po metodi združevanja najbližjih sosedov (NJ) z manjšim odstopanjem za akcesiji ACC6 in ACC28. Genotipi akcesije ACCmk so se genetsko najbolj razlikovali od drugih na osnovi največjega števila prisotnih namnožkov (241) in največjega števila prisotnih “privatnih namnožkov” (10) in v številu namnožkov, ki so bili omejeni samo na eno populacijo.
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DOI: http://dx.doi.org/10.14720/aas.2022.118.3.2344
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Avtorske pravice (c) 2022 Barbara PIPAN, Sali ALIU, Dukagjin ZEKA, Vladimir MEGLIČ
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