In vitro protiglivni potencial surfaktina, izoliranega iz bakterije Bacillus thuringiensis Berliner 1915 iz rizosfere koruze (Zea mays L.) proti patogeni glivi Fusarium graminearum Schwabe

Muddasir KHAN, Muhammad SALMAN, Syed Hussain SHAH, Muhammad ISRAR

Povzetek


Gliva Fusarium graminearum povzroča znantne izgube v pridelku koruze in drugih žit širom po svetu. Uporaba kemičnih sredstev za zatiranje povzroča resne okoljske probleme. Vrste iz rodu Bacillus in druge rast vzpodbujajoče bakterije (PGPR) igrajo ključno vlogo pri razvoju biopesticidov. Proizveden je bil že širok spekter okolju prijaznih antimikrobnih agensov. Raziskava se osredotoča na uporabo protiglivne aktivnosti lipopeptidnih surfaktinov iz bakterije Bacillus thuringiensis proti patogeni glivi Fusarium graminearum. Bakterija B. thuringensis je bila izolirana iz rizosfere posevka koruze in gojena za proizvodno lipopeptidov. Surfaktin je bil določen s tekočinsko kromatografijo visoke ločljivosti (HPLC) iz izvlečka pri 210 nm, retencijskim časom 3-5 minut, dobljeni višek je bil 3.990. Rast patogene glive je bila uspešno zavrta pri različnih koncentracijah surfaktina. 80 % koncentracija surfaktina je pokazala največjo sposobnost zaviranja v primerjavi s koncentracijami 60 %, 40 % in 20 % (p < 0,005). Na osnovi te raziskave lahko zaključimo, da ima lipopeptidni surfaktin iz bakterije B. thuringensis velik potencial za zaviranje rasti glive F. graminearum.


Ključne besede


surfaktin; Bacillus; biološka kontrola; HPLC; Fusarium graminearum

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2021.117.4.2345

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