UČINEK RASTLINSKIH RASTNIH REGULATORJEV NA RAST IN NEPOSREDNO TVORBO POGANJKOV IZ LISTNIH IZSEČKOV PRI KRIŽANCU Phalaenopsis ‘Pink’

Avishek DATTA, Meutia ZAHARA, Patchareeya BOONKORKAEW, Abha MISHRA

Povzetek


Orhideje iz rodu Phalaenopsis so med najlepšimi cvetnicami. Namen te raziskave je bil ugotoviti najprimernejšo kombinacijo rastlinskih rastnih regulatorjev in gojišč za rast in neposredno tvorbo poganjkov iz listnih izsečkov križanca Phalaenopsis ‘Pink’. Izsečki vrhnjih delov lista mladih, in vitro vzgojenih rastlin so bili gojeni na polovičnem Murashige in Skoog (MS) ter Vacin in Went (VW) gojišču, in dodane so bile različne koncentracije auksina [α-naftalenocetna kislina (NAA)] in citokinina [6-benzilaminopurin (BAP) ali tidiazurona (TDZ)]. Izsečki, ki so bili gojeni na polovičnem MS gojišču z dodatkom 0 mg l–1 NAA in 3 mg l–1 BAP so v desetih tednih uspešno tvorili poganjke s 5 % nastalih regenerantov in 50 % preživetjem. Izsečki, ki so bili gojeni na polovičnem MS gojišču z dodatkom 0.5 mg l–1 NAA in 1.5 mg l–1 TDZ so tvorili kalus in poganjke v enajstih tednih s 25 % nastalih regeneratov in 90 % preživetjem. Potrebne so še nadaljnje raziskave za določitev najkrajšega časa regeneracije poganjkov, ki bi dale vitalne rastline z dobrim preživetjem.

Ključne besede


Phalaenopsis; tvorba poganjkov; α-naftalenocetna kislina; 6-benzilaminopurin; tidiazuron

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2018.111.1.01

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