Primerjalno preverjanje ISSR, DAMD in RAPD markerjev za vrednotenje genetske raznolikosti sort gerbere (Gerbera jamesonii Bolus ex Hooker f.)

Abbas SAIDI, Melika HAJKAZEMIAN

Povzetek


Genetska raznolikost je izhodišče razpoložljivih genetskih virov v žlahtniteljskih programih gerbere. V študiji smo genetsko raznolikost 22 sort gerbere preučevali z označevalci ISSR (inter-simple sequence repeat, regije med mikrosateliti), DAMD (direct amplified minisatellite DNA, pomnoženi minisateliti) in RAPD (Random Amplified Polymorphic DNA, naključno pomnožena polimorfna DNA). Popreprečna informacijska vrednost polimorfizma (PIC) je bila za ISSR, DAMD in RAPD markerje 0,40; 0,41 in 0,40. Klasterska analiza ISSR, DAMD in RAPD markerjev je sorte razdelila v tri različne skupine. Primerjalna analiza treh markerjev (ISSR, DAMD in RAPD) je pokazala, da so imajo DAMD markerji prednost pred RAPD in ISSR za določitev genetske raznolikosti pri gerberi. Pričujoča raziskava je ena prvih, ki primerja učinkovitost DAMD, ISSR in RAPD markerjev na izbranem naboru genotipov gerber. Naši rezultati so pokazali, da so markerji DAMD najbolje prikazali raznolikost genotipov gerbere.


Ključne besede


Gerbera; DAMD; genetska raznolikost; ISSR; DAMD

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2023.119.1.2425

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Avtorske pravice (c) 2023 Abbas SAIDI, Melika HAJKAZEMIAN

 

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