Določanje genetske raznolikosti čilija (Capsicum annuum L.) z mikrosateliti: Ovrednotenje genetskega materiala v Bangladešu

Md. Rezwan MOLLA, Iftekhar AHMED, Md. Motiar ROHMAN, Mohammad Amdadul HAQUE, Shah Md. Monir HOSSAIN, Lutful HASSAN

Povzetek


Analiza genetske raznolikosti je predpogoj za vzgojo izboljšanih sort katerekoli kulturne rastline. Zatradi tega je bilo uporabljenih 39 SSR lokusov za analizo genetske raznolikosti genotipov čilija. S PCR pomnoženi mikrosatelitski lokusi so bili polimorfni pri vseh preučenih genotipih. Pri lokusu CAMS-647 smo zaznali največje število alelov (8), ki so obsegali dolžine od 188 do 279 bp. PIC vrednosti so za 39 začetnih oligonukleotidov (primerjev) znašale od 0,099 za lokus Hpms 1-165 do 0,806 za lokus CAMS-679. Vsi analizirani mikrosateliti (SSR) so bili za vrednotenje genenotipske variabilnosti vzorcev informativni, med njimi jih je bilo 12 z večjimi PIC vrednostmi (> 0,6) najprimernejših. Genetska raznolikost je bila velika in je variirala od 0,117 (HpmsE075) do 0,806 (CAMS-647), največja (1,713) in najmanjša (0,205) vrednost Shannonovega informacijskega indeksa sta bili ugotovljeni za lokusa CAMS-679 in Hpms 1-165. Ugotovljena je bila visoka stopnja genetske diferenciacije (0,927) in majhen pretok genov (0,010). Neijeva genetska distanca je variirala med 0,100 in 0,990. Med 96 genotipi jih je imelo 55 jasen položaj v dendrogramu z večjimi vrednostmi genske distance (> 0,6) medtem, ko je 41 genotipov pokazalo ožjo sorodnost z manjšimi v rednostmi genske distance.


Ključne besede


čili; genetska raznolikost; microsatelistki markerji (SSR); informacijska vrednost polimorfizma

Celotno besedilo:

PDF (English)

Literatura


Abouzied, H. M., Eldemery, S. M. M., & Abdellatif, K. F. (2013). SSR-based genetic diversity assessment in tetraploid and hexaploid wheat populations. Biotechnology Journal International, 390–404. https://doi.org/10.9734/BBJ/2013/4340

Anumalla, M., Roychowdhury, R., Geda, C. K., Mazid, M., & Rathoure, A. K. (2015). Utilization of plant genetic resources and diversity analysis tools for sustainable crop improvement with special emphasis on rice. International Journal of Advanced Research, 3(3), 1155–1175. https://doi.org/http://www.journalijar.com/article/4175/utilizationofplantgeneticresourcesanddiversityanalysistoolsforsustainablecropimprovementwithspecialemphasisonrice/

BBS. (2021). Statistical Year Book Bangladesh.

Cao, T., Duprez, E., Borden, K. L. B., Freemont, P. S., & Etkin, L. D. (1998). Ret finger protein is a normal component of PML nuclear bodies and interacts directly with PML. Journal of Cell Science, 111(10), 1319–1329. https://doi.org/10.1242/jcs.111.10.1319

Chen, X., Min, D., Yasir, T. A., & Hu, Y. G. (2012). Genetic diversity, population structure and linkage disequilibrium in elite chinese winter wheat investigated with SSR markers. PLoS ONE, 7(9), 1–9. https://doi.org/10.1371/journal.pone.0044510

Dhaliwal, M. S., Yadav, A., & Jindal, S. K. (2014). Molecular characterization and diversity analysis in chilli pepper using simple sequence repeats (SSR) markers. African Journal of Biotechnology, 13(31), 3137–3143. https://doi.org/10.5897/ajb2014.13695

Di Dato, F., Parisi, M., Cardi, T., & Tripodi, P. (2015). Genetic diversity and assessment of markers linked to resistance and pungency genes in Capsicum germplasm. Euphytica, 204(1), 103–119. https://doi.org/10.1007/s10681-014-1345-4

Farhad, M., Hasanuzzaman, M., Biswas, B. K., Arifuzzaman, M., & Islam, M. M. (2010). Genetic divergence in chilli (Capsicum annuum L.). Bangladesh Research Publication Journal, 3(3), 1045–1058.

Hernández-Pérez, T., Gómez-García, M. del R., Valverde, M. E., & Paredes-López, O. (2020). Capsicum annuum (hot pepper): An ancient Latin-American crop with outstanding bioactive compounds and nutraceutical potential. A review. Comprehensive Reviews in Food Science and Food Safety, 19(6), 2972–2993. https://doi.org/10.1111/1541-4337.12634

Hossain, S. M., Habiba, U., Bhuyan, S. I., Haque, M. S., Begum, S. N., & Hossain, D. M. (2014). DNA fingerprinting and genetic diversity analysis of chilli germplasm using microsatellite markers. Biotechnology, 13(4), 174–180. https://doi.org/10.3923/biotech.2014.174.180

Islam, M. N., Molla, M. R., Rohman, M. M., Hasanuzzaman, M., Islam, S. M. N., & Rahman, L. (2012). DNA fingerprinting and genotyping of cotton varieties using SSR markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(2), 261–265. https://doi.org/10.15835/nbha4028166

Islam, M. Z., Khalequzzaman, M., Bashar, M. K., Ivy, N. A., Mian, M. A. K., Pittendrigh, B. R., Haque, M. M., & Ali, M. P. (2018). Variability assessment of aromatic rice germplasm by pheno-genomic traits and population structure analysis. Scientific Reports, 8(1), 1–14. https://doi.org/10.1038/s41598-018-28001-z

Jain, S., Kumar, A., Mamidi, S., & McPhee, K. (2014). Genetic diversity and population structure among pea (Pisum sativum L.) cultivars as revealed by simple sequence repeat and novel genic markers. Molecular Biotechnology, 56(10), 925–938. https://doi.org/10.1007/s12033-014-9772-y

Lee, C. J., Yoo, E. Y., Shin, J., Lee, J., Hwang, H. S., & Kim, B. D. (2005). Erratum: Non-pungent Capsicum contains a deletion in the capsaicinoid synthetase gene, which allows early detection of pungency with SCAR markers (Molecules and Cells 19, 2 (262-267)). Molecules and Cells, 20(2), 303.

Mimura, Y., Inoue, T., Minamiyama, Y., & Kubo, N. (2012). An SSR-based genetic map of pepper (Capsicum annuum L.) serves as an anchor for the alignment of major pepper maps. Breeding Science, 62(1), 93–98. https://doi.org/10.1270/jsbbs.62.93

Minamiyama, Y., Tsuro, M., & Hirai, M. (2006). An SSR-based linkage map of Capsicum annuum. Molecular Breeding, 18(2), 157–169. https://doi.org/10.1007/s11032-006-9024-3

Molla, K. A., Bhusan, A. B., Ganie, S. A., & Mondal, T. K. (2015). Identification and analysis of novel salt responsive candidate gene based SSRs (cgSSRs) from rice (Oryza sativa L.). BMC Plant Biology, 15(1), 1–11. https://doi.org/10.1186/s12870-015-0498-1

Molla, M. R., Ahmed, I., Rahman, S., Hossain, M. A., Salam, M. A., Chowdhury, M. A. Z., & Rohman, M. M. (2017). Genetic diversity among muskmelon (Cucumis melo L.) germplasm in Bangladesh as revealed by microsatellite markers. African Journal of Agricultural Research, 12(44), 3203–3213. https://doi.org/10.5897/ajar2017.12617

Molla, M. R., Ahmed, I., Rohman, M. M., Hossain, M. A., & Chowdhury, M. A. Z. (2016). Genetic diversity analysis and DNA fingerprinting of Mungbean (Vigna radiata L.) genotypes using SSR markers. Plant Science, 6(4), 153–164.

Molla, Md Rezwan, Rohman, M. M., Monsur, M. B., Hasanuzzaman, M., & Hassan, L. (2021). Screening and assessment of selected chilli (Capsicum annuum L.) genotypes for drought tolerance at seedling stage. Phyton, 90(5), 1425. https://doi.org/10.32604/phyton.2021.015591

Nei, M. (1972). Genetic distance between populations. The American Naturalist, 106(949), 283–292. https://doi.org/10.1086/282771

Pickersgill, B. (1991). Cytogenetics and evolution of Capsicum L. In: Tsuchiya T, Gupta PK (Editors), Chromosome Engineering in Plants: Genetics, Breeding, Evolution Part B. Elsevier, Amsterdam. pp. 139-160. https://doi.org/10.1016/B978-0-444-88260-8.50013-6

Rahman, M., Sohag, M., & Rahman, L. (2010). Microsatellite based DNA fingerprinting of 28 local rice (Oryza sativa L.) varieties of Bangladesh. Journal of the Bangladesh Agricultural University, 8(1), 7–17. https://doi.org/10.3329/jbau.v8i1.6391

Roychowdhury, R., Taoutaou, A., Hakeem, K. R., Ragab, M., Gawwad, A., & Tah, J. (2014). Molecular Marker-Assisted Technologies for Crop Improvement. In Crop improvement in the era of climate change (Vol. 2, Issue April 2016, pp. 241–258). https://doi.org/10.13140/RG.2.1.2822.2560

Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A., & Allard, R. W. (1984). Ribosomal DNA spacer-length polymorphisms in barley : Mendelian inheritance , chromosomal location , and population dynamics. Proceedings of National Academy Science, 81(December), 8014–8018. https://doi.org/10.1073/pnas.81.24.8014

Sharmin, A., Hoque, M. E., Haque, M. M., & Khatun, F. (2018). Molecular diversity analysis of some chilli (<i>Capsicum</i> spp.) genotypes using SSR markers. American Journal of Plant Sciences, 09(03), 368–379. https://doi.org/10.4236/ajps.2018.93029

Sundaram, R. M., Naveenkumar, B., Biradar, S. K., Balachandran, S. M., Mishra, B., Ilyasahmed, M., Viraktamath, B. C., Ramesha, M. S., & Sarma, N. P. (2008). Identification of informative SSR markers capable of distinguishing hybrid rice parental lines and their utilization in seed purity assessment. Euphytica, 163(2), 215–224. https://doi.org/10.1007/s10681-007-9630-0

van Zonneveld, M., Scheldeman, X., Escribano, P., Viruel, M. A., van Damme, P., Garcia, W., Tapia, C., Romero, J., Sigueñas, M., & Hormaza, J. I. (2012). Mapping genetic diversity of cherimoya (Annona cherimola Mill.): Application of spatial analysis for conservation and use of plant genetic resources. PLoS ONE, 7(1). https://doi.org/10.1371/journal.pone.0029845

Yi, G., Lee, J. M., Lee, S., Choi, D., & Kim, B. D. (2006). Exploitation of pepper EST-SSRs and an SSR-based linkage map. Theoretical and Applied Genetics, 114(1), 113–130. https://doi.org/10.1007/s00122-006-0415-y




DOI: http://dx.doi.org/10.14720/aas.2022.118.4.2511

Povratne povezave



Avtorske pravice (c) 2022 Md. Rezwan MOLLA, Iftekhar AHMED, Md. Motiar ROHMAN, Mohammad Amdadul HAQUE, Shah Md. Monir HOSSAIN, Lutful HASSAN

 

Acta agriculturae Slovenica je odprtodostopna revija, ki objavlja pod pogoji licence Creative Commons Priznanje avtorstva (CC BY).

                     


eISSN 1854-1941