Predtretiranje semen s cinkovimi nano delci je zmanjšalo izražanje genov tolerance na slanost pri soji (Glycine max L.) in izboljšalo lastnosti pridelka

Reda Mohamed GAAFAR, Mohamed Lotfi HALAWA, Adel Ramadan EL-SHANSHORY, Abdelhamid Abdelrahim EL-SHAER, Rana Hosny DIAB, Marwa Mahmoud HAMOUDA

Povzetek


Malo je bilo narejenega za ovrednotenje molekularne vloge nano delcev ZnO (ZNPs) pri uravnavanju biokemičnih procesov in pridelka rastlin kot odziva na slanostni stres. V tej raziskavi je bil ocenjen molekularni odziv na solni stres pri soji (‘Giza111’) pri uporabi različnih koncentracij ZNPs (25, 50, 100, in 200 mg l-1) z meritvami nekaterih osmotikov, parametrov pridelka in vsebnosti Na+ in K+. Vpliv slanosti na količino mRNK treh ključnih s toleranco na slanost povezanih genov (GmCHX1, GmPAP3, in GmSALT3) je bil določen z uporabo qRT-PCR metode. Velika slanost (250 mM NaCl) je vodila k znatnemu povečanju vsebnosti Na+, celokupnih topnih beljakovin, celokupnih topnih ogljikovih hidratov in značilno povečala izražanje genov GmCHX1, GmPAP3, in GmSALT3, med tem ko, je zmanjšala vsebnost K+, razmerja K+/Na+ in vse parameter pridelka v primerjavi s kontrolo. Namakanje semen soje v različnih koncentracijah ZNP je povečalo vsebnost K+ in razmerje K+/Na+ v rastlinah pod stresom in hkrati zmanjšalo vsebnost Na+, celokupnih topnih beljakovin in celokupnih topnih ogljikovih hidratov, še posebej pri uporabi 50 mg l-1 ZNPs. Dodatno je bilo pri tem obravnavanju zmanjšano izražanje genov GmCHX1, GmPAP3,in GmSALT3, kar je na koncu izboljšalo parametre pridelka soje. Skladno s temi rezultati priporočamo uporabo 50 mg l-1 ZNPs za izboljšanje pridelka soje, gojene na slanih tleh.


Ključne besede


ZnO; nano delci; slanost; soja; izražanje genov; qRT-PCR; produktivnost

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2022.118.3.2529

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Avtorske pravice (c) 2022 Reda Mohamed GAAFAR, Mohamed Lotfi HALAWA, Adel Ramadan EL-SHANSHORY, Abdelhamid Abdelrahim EL-SHAER, Rana Hosny DIAB, Marwa Mahmoud HAMOUDA

 

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