Učinki γ-sevanja na sorte čičerke (Cicer arietinum L.) in njihova toleranca na slanostni stres
Povzetek
Čičerka (Cicer arietinum L.) je obojespolna samoprašna stročnica. Zaradi sposobnosti vezave atmosferskega dušika s simbiontskimi bakterijami izboljšuje rodovitnost tal. Slanost je eden izmed najpomembnejših abiotskih stresnih dejavnikov, ki vpliva na rast rastlin. V raziskavi je bil ovrednoten vpliv γ-sevanja na kalitev, celične delitve in rast rastlin F1 generacije čičerke. Semena sedmih sort čičerke so bila obsevana z γ-žarki v jakosti od 50 Gy do 600 Gy. V primerjavi s kontrolo so bile zabeležene neznačilne razlike v odstotku kalitve pri semenih, ki so bila izpostavljena 50 Gy, 100 Gy in 200 Gy γ-sevanja, razen pri sorti ILC 484. V primerjavi s kontrolo se je pri vseh sortah povečal mitotski indeks (MI) celic rastnega vršička korenine, ki so bile obsevane z majhnimi dozami 50 Gy, 100 Gy in 200 Gy ter zmanjšal pri obsevanju z večjimi dozami. Vse uporabljene doze γ-sevanja so vzpodbudile različen obseg kromosomskih aberacij. Najbolj pogoste so bile mostički, zaostali kromosomi in zlepljeni kromosomi v metafazah ter zlomljeni kromosomi, mikronukleusi in dvojedrne celice po delitvi. Doze sevanja z jakostjo od 300 Gy do 600 Gy so vzpodbudile razpad jedrnih membran. Slanostna obravnavanja s 25 mM NaCl in 60 mM NaCl so zmanjšala rast sejank vseh sort. Doza obsevanja s 100 Gy je zmajšala učinek slanostnega stresa 25 mM NaCl pri večini sort, razen pri sortah FLIP 84-188 in FLIP 97-263. Obravnavanje z dozo 60 mM NaCl je značilno zmanjšalo zgodnjo rast pri vseh sortah in negativnega učinka ni bilo mogoče zmanjašati z γ-obsevanjem.
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DOI: http://dx.doi.org/10.14720/aas.2022.118.2.2538
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