Pošlji prispevek (e-pošta) Genetska spremenljivost odziva navadnega fižola (Phaseolus vulgaris L.) na sušni stres je povezana z biokemičnim in transkripcijskim uravnavanjem presnove ureidov
Povzetek
Ureidne stročnice kot je navadni fižol (Phaseoulus valgaris L.) transportirajo med simbiontsko vezavo dušik iz nodulov v liste kot ureide. Pri navadnem fižolu je pomemben gen, ki kodira alantoinazo (alantoin amidohidrolaza, EC 3.5.2.5), ključni encim, ki katalizira hidrolizo alantoina v alantoinsko kislino. Ta ima pomembno vlogo pri tvorbi ureidov za njihov eksport in razgradnjo kot vir dušika v tkivih ponora. Pri rastlinah je eden izmed prilagoditvenih mehanizmov na sušni stres povezan s kopičenjem ureidov. Za določitev genetske variabilnosti navadnega fižola na sušni stres so bile analizirane spremembe v izražanju gena za alantoinazo, ALLANTONAISE (PvALN) in vsebnosti ureidov v listnih tkivih pri treh genotipih navadnega (CAL96, DAB514 and DAB541) in enem genotipu ostrega fižola, Phaseolus acutifolius A.Gray. Med vsemi genotipi bi občutljivost genotipa DAB514 navadnega fižola verejtno lahko pripisali zavrtju izražanja gena PvALN, kar je bilo povezano z zmanjšano tvorbo ureidov in slabšo rastjo. V nasprotju je sušni stres vzpodbudil povečano izražanje tega gena, kar je bilo povezano s povečanjem vsebnosti alantoina in alantoata pri genotipu DAB541. Dodatno bi ohranjeno rast genotipa CAL96 lahko pripisali stalni količini allantoina, ki se sintetizira med sušnim stresom. Zaključimo lahko, da sta genotipa navadnega fižola DAB541 in CAL96 obetajoča, z vzpodbujeno povečano transkripcijsko kontrolo katabolizma in/ali biosinteze ureidov, ki bi lahko služila kot potencial za izbor in uvajanje ustreznih genotipov v sušnih razmerah Botswane.
Ključne besede
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PDF (English)Literatura
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DOI: http://dx.doi.org/10.14720/aas.2022.118.2.2541
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