Pošlji prispevek (e-pošta) Somatska embriogeneza in vzgoja rastlin iz radikule zigotskih embrijev oljke (Olea europaea ‘Chemlal’)
Povzetek
Izboljšanje oljke z biotehnološkimi orodji kot jer genetska transformacija zahteva učinkovit in vitro sistem vzgoje rastlin. V tem pogledu se zdi somatska embriogeneza najprimernejši način vzgoje. Raziskava opisuje prvič vzgojo rastlin najvažnejše sorte oljke Chemlal v Alžiriji s somatsko embriogenezo iz radikule zrelih zigotskih embrijev. Rezultati so pokazali, da je vzpostavitev primerne embriogene kulture močno odvisna od kemične sestave gojišča, v katerem poteka indukcija kalusa in gojišča za njegovo vzdrževanje kot tudi od dodatka rastnih regulatorjev. Več kot 10 in 13 % nodularnih kalusov je bilo pridobljenih na trdih MS in OMc gojiščih, ki sta vsebovali IBA in zeatin, čemur je sledil prenos v isto gojišče brez zeatina in zmanjšano koncentracijo auksina, 3,3 in 6,7 % embriogeneze pa je bilo dosežene z IAA na MS gojišču in dodatkom NAA na obeh preiskušenih gojiščih. Nasprotno pa ni bilo embriogeneze pri dodatku 2,4-D ali pri kontroli, kjer je bila indukcija kalogeneze slabša. Naknadno je ECO gojišče z IBA, zeatinom in BA, še posebej v tekoči kulturi, omogočilo boljšo proliferacijo in pojav embriogeneze v primerjavi z OM in MS gojiščem. Na koncu so zreli somatski embriji hitro vzkalili na trdnem OM osnovnem gojišču in dali normalne, dobro razvite rastlinice, ki so se zlahka prilagodile naravnim razmeram.
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DOI: http://dx.doi.org/10.14720/aas.2022.118.4.2581
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