RAZNOLIKOST KLONSKIH KANDIDATOV Vitis vinifera 'ŠKRLET BIJELI' V AROMATIČNIH SNOVEH, DOLOČENIH S PLINSKO KROMATOGRAFIJO-MASNO SPEKTROSKOPIJO

Ivana Vladimira Petric, Tatjana Košmerl, Ivan Pejić, Veronika Kubanović, Emil Zlatić

Povzetek


Namen tega dela je bil ugotoviti prisotnost in stabilnost specifičnih aromatičnih spojin mošta (monoterpeni C13-norizoprenoidi, C6-alkoholi, alkoholi, estri in karbonilne spojine) ter katere od teh spojin se lahko uporabljajo za razlikovanje klonskih kandidatov sorte Vitis vinifera 'Škrlet bijeli'. V ta namen smo spojine, ki so odgovorne za sortni aromatični profil, identificirali s plinsko kromatografijo-masno spektrometrijo (GC-MS), v vzorcih mošta desetih klonskih kandidatov, ki rastejo na dveh lokacijah, v treh zaporednih letnikih. Za določanje razlik med kloni in opredelitev spojin, ki so odgovorne za te razlike, smo uporabili multivariatne statistične metode (analizo glavnih komponent in linearno diskriminantno analizo). Značilne razlike med klonskimi kandidati so se pokazale v 22 od skupno 35 identificiranih aromatičnih spojin. Za aromatski profil klona smo ugotovili prevladujoč vpliv lokacije vinograda. Razlike v primarnih aromatičnih spojinah, odgovornih za aromo 'Škrlet Bijeli', linaloola, terpinolena, nerola in α-terpineola, niso bile statistično značilne med klonskimi kandidati, medtem ko so bile določene pomembne razlike v β-damascenonu. V nasprotju s pričakovanji, monoterpena geraniola nismo določili. Druge določene aromatične spojine (trans-ocimen, 2-metil-1-butanol, mircen, α-felandren, cis-ocimen in 3-metil-1-butanol) občutno manj sodelujejo pri skupnem opisu sortne arome, vendar še vedno omogočajo razlikovanje klonskih kandidatov.


Ključne besede


klonska selekcija, mošt, aromatične spojine, plinska kromatografija-masna spektrometrija (GC-MS), multivariatna statistična analiza

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2016.107.2.19

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